Establishment of a Tongue Squamous Cell Carcinoma Cell Line from Indian Gutka Chewer

Patil, Tejas; Kowtal, Pradnya; Nikam, Abhijeet; Barkume, Madan; Patil, Asawari; Kane, Shubhada; Juvekar, Aarti; Mahimkar, Manoj B.; Kayal, J. J.

doi:10.1155/2014/286013

Cited by 12 publications

(15 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Human tissue sampling was approved by the Changhua Christian Hospital Institutional Review Board and written informed consent from the patients. The normal oral epithelial cells were isolated and cultured as described previously by Patil et al [28]. The tissues were cut into smaller pieces and incubated with trypsin (0.025%) for 30 min, at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Cantharidin Induced Oral Squamous Cell Carcinoma Cell Apoptosis via the JNK-Regulated Mitochondria and Endoplasmic Reticulum Stress-Related Signaling Pathways

Lee

Chen

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

Oral cancer is a subtype of head and neck cancer which represents 2.65% of all human malignancies. Most of oral cancer is histopathologically diagnosed as oral squamous cell carcinoma (OSCC). OSCC is characterized by a high degree of local invasion and a high rate of metastasis to the cervical lymph nodes. How to prevention and treatment of OSCC is important and imperative. Here, we investigated the therapeutic effect and molecular mechanism of cantharidin, an active compound isolated from blister beetles, on OSCC in vitro. Results showed that cantharidin significantly decreased cell viability in human tongue squamous carcinoma-derived SAS, CAL-27, and SCC-4 cell lines. The further mechanistic studies were carried out in SAS cells. Cantharidin also significantly increased apoptosis-related signals, including caspase-9, caspase-7 and caspase-3 proteins. Besides, cantharidin decreased mitochondrial transmembrane potential (MMP) and induced cytochrome c and apoptosis inducing factor (AIF) release. Cantharidin also increased Bax, Bid, and Bak protein expressions and decreased Bcl-2 protein expression. Cantharidin could also increase the endoplasmic reticulum (ER) stress signals, including the expressions of phosphorylated eIF-2α and CHOP, but not Grp78 and Grp94. Furthermore, cantharidin reduced pro-caspase-12 protein expression. In signals of mitogen-activated protein kinases, cantharidin increased the phosphorylation of JNK, but not ERK and p38. Transfection of shRNA-JNK to OSCC cells effectively reversed the cantharidin-induced cell apoptotic signals, including the mitochondrial and ER stress-related signaling molecules. Taken together, these findings suggest that cantharidin induces apoptosis in OSCC cells via the JNK-regulated mitochondria and ER stress-related signaling pathways.

show abstract

Section: Methodsmentioning

confidence: 99%

Cantharidin Induced Oral Squamous Cell Carcinoma Cell Apoptosis via the JNK-Regulated Mitochondria and Endoplasmic Reticulum Stress-Related Signaling Pathways

Lee

Chen

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…In addition, cell lines are required for evaluating the effects of numerous novel drugs and finding better treatments (Kaur and Ralhan, 2003). Several studies identified that the wide variety of patients who suffered from tongue cancer were smokers and most cell lines were determined from them, whereas few cell lines recognized from tumors of nonsmokers (Patil et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

EZB-ICR Cell Line: A New Established and Characterized Oral Squamous Cell Carcinoma Cell Line From Tongue

Chenari

Khademi

Razmkhah

2021

Asian Pac J Cancer Prev

View full text Add to dashboard Cite

Background: Tongue cancer is one of the most aggressive forms of oral squamous cell carcinoma which needs more investigations. Herein, we aimed to establish and characterize a tongue cancer cell line. Methods: Tumor tissue was obtained from a 70-year-old woman with tongue cancer. The established cell line named as EZB-ICR and characterized for doubling time, expression of specific markers, HPV corporation and migration status using flow cytometry, immunofluorescence staining, multiplex PCR, and migration assay. Results: EZB-ICR was negative for expression of mesenchymal specific markers, cytokeratin19, pan-cytokeratin, vimentin and EPCAM, but was positive for S100 and Nestin. No appearance of human papilloma virus DNA was seen. The doubling time of EZB-ICR was 31 hours and migration assay confirmed its metastatic nature. Conclusion: To the best of our knowledge, EZB-ICR is the first tongue human cancer cell line established in Iran, and its features make it appropriate for cancer-based in vitro studies and contribute to more studies on tongue cancer.

show abstract

“…The establishment of HNSCC cell lines from Indian patients has previously been achieved from carcinomas of tongue, alveolus and retromolar trigone (24,25); however, these cell lines did not possess the ability to give rise to tumors when injected into immune-compromised mice (24,25). Furthermore, the establishment of a cell line from carcinoma of the upper aerodigestive tract, which can give rise to tumors when subcutaneously xenografted in nude mice was reported (26).…”

Section: Establishment and Characterization Of Novel Human Oral Squamous Cell Carcinoma Cell Lines From Advanced-stage Tumors Of Buccal Mmentioning

confidence: 99%

Establishment and characterization of novel human oral squamous cell carcinoma cell lines from advanced‑stage tumors of buccal mucosa

et al. 2019

View full text Add to dashboard Cite

Oral squamous cell carcinoma (OSCC) is a leading cause of mortality in India owing to the high percentage of tobacco chewers, smokers and alcohol consumption. OSCC is highly heterogeneous in nature; therefore poses a challenge in the treatment of the patient. To better understand the heterogeneity of the tumors, an in vitro cell line model is required. However, the efficiency of establishing cell lines from the oral tumors is low. In the present study, three novel cell lines, namely ACOSC3, ACOSC4, and ACOSC16, were isolated and characterized from advanced-stage treatment-naive OSCCs originating from the buccal mucosa. The three cell lines exhibited polygonal morphology, which is typical of epithelial cells. Furthermore, immunofluorescence revealed the expression of keratins 8 and 14, thereby confirming the epithelial origin of the cells. DNA content analysis of the three OSCC cell lines revealed aneuploidy. Furthermore, an in vitro orosphere assay revealed the formation of primary orospheres. Notably, the OSCC cell lines were able to give rise to tumors when administered subcutaneously into non-obese diabetic/severe combined immune deficiency mice. The novelty of the cell lines was also validated by performing short tandem repeat profiling; the STR profiles of the present cell lines did not significantly match with any known established OSCC cell lines present in the DSMZ database, thereby confirming the unique identity of these lines. These cell lines established from tumor samples derived from Indian OSCC patients provide a valuable resource to understand the molecular mechanism involved in tumor resistance and recurrence.

show abstract

Establishment of a Tongue Squamous Cell Carcinoma Cell Line from Indian Gutka Chewer

Cited by 12 publications

References 33 publications

Cantharidin Induced Oral Squamous Cell Carcinoma Cell Apoptosis via the JNK-Regulated Mitochondria and Endoplasmic Reticulum Stress-Related Signaling Pathways

Cantharidin Induced Oral Squamous Cell Carcinoma Cell Apoptosis via the JNK-Regulated Mitochondria and Endoplasmic Reticulum Stress-Related Signaling Pathways

EZB-ICR Cell Line: A New Established and Characterized Oral Squamous Cell Carcinoma Cell Line From Tongue

Establishment and characterization of novel human oral squamous cell carcinoma cell lines from advanced‑stage tumors of buccal mucosa

Contact Info

Product

Resources

About