Establishment of Agrobacterium-mediated genetic transformation system in Dahlia

Abstract

An efficient system for Agrobacterium-mediated transformation was established in dahlia (Dahlia×pinnata) 'Yamatohime' . Mass of shoot primordia (MSP) induced on MS medium supplemented with 10 mg l −1 TDZ were inoculated with Agrobacterium tumefaciens strain EHA101 (pIG121-Hm) harboring both β-glucuronidase (GUS) and hygromycin resistant genes. After 2 days of co-cultivation, the MSP were transferred to a selection medium containing hygromycin with meropenem for bacterial elimination. Shoots were successfully regenerated from survived MSP on hormone-free medium without hygromycin and they rooted on hormone-free medium containing hygromycin. The hygromycin-resistant plants thus obtained showed histochemical blue staining for GUS. Transformation of plants was confirmed by PCR and Southern blot analyses. Transgenic 'Kokucho' were also produced by using the same transformation procedure, suggesting wide applicability of this Agrobacterium-mediated transformation procedure for other dahlia cultivars.

Ectopic Expression of PAP1 Leads to Anthocyanin Accumulation and Novel Floral Color in Genetically Engineered Goldenrod (Solidago canadensis L.)

“…Transformation mediated by A. tumefaciens has been successfully applied to numerous Asteraceae family members, e.g., sunflower, lettuce, chrysanthemum, and dahlia (Muller et al, 2001; Weber et al, 2003; Mohebodini et al, 2011; Otani et al, 2013; Noda et al, 2017). In the genus Solidago , successful transformation has only been reported for Solidago nemoralis , using Agrobacterium rhizogenes for the generation of hairy root cultures (Gunjan et al, 2013).…”

Ultra-Sonic Sound Applications Used in Seed Viability, Seedling Growth and Plant Development of Ornamentals

“…Ultra-sonic sound applications on some ornamental plant species were performed in vitro and their effects on root growth and callus development in general were investigated [(Gerbera jamesonii) (Wang et al 2003), (Aloe arborescens Mill.) (Liu et al 2003), (Dendrobium officinale) (Wei et al 2012), (Dahlia x pinnata) (Otani et al 2013)].…”