Establishment of an Aqueous PEG 200-Based Deep Eutectic Solvent Extraction and Enrichment Method for Pumpkin (Cucurbita moschata) Seed Protein

Liu, Ruilin; Yu, Pei; Ge, Xian-Li; Xiu-feng, Bai; Li, Xing-Qiang; Fu, Qiang

doi:10.1007/s12161-016-0732-y

Cited by 59 publications

(23 citation statements)

References 38 publications

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“…Foods 2020, 9, x FOR PEER REVIEW 3 of 32 (Treculia Africana) [56], pumpkin (Cuburbita pepo [57][58][59][60][61][62] and Cucurbita moschata [20,32,63,64]), and date (Phoenix dactylifera) [65,66]. Some of these residues are released in the extraction of the oil fraction of seeds (such as pumpkin seeds or rapeseed) and of fruits (such as olives) or during the processing of fruits or vegetables such as pomegranate, Prunus fruits, tomato, muskmelon, watermelon, papaya, and mango.…”

Section: Obtaining Antioxidant Proteins and Peptides From Fruit Residuesmentioning

confidence: 99%

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Extraction and Characterization of Antioxidant Peptides from Fruit Residues

Olivares-Galván

Marina

García

2020

Foods

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Fruit residues with high protein contents are generated during the processing of some fruits. These sustainable sources of proteins are usually discarded and, in all cases, underused. In addition to proteins, these residues can also be sources of peptides with protective effects against oxidative damage. The revalorization of these residues, as sources of antioxidant peptides, requires the development of suitable methodologies for their extraction and the application of analytical techniques for their characterization. The exploitation of these residues involves two main steps: the extraction and purification of proteins and their hydrolysis to release peptides. The extraction of proteins is mainly carried out under alkaline conditions and, in some cases, denaturing reagents are also employed to improve protein solubilization. Alternatively, more sustainable strategies based on the use of high-intensity focused ultrasounds, microwaves, pressurized liquids, electric fields, or discharges, as well as deep eutectic solvents, are being implemented for the extraction of proteins. The scarce selectivity of these extraction methods usually makes the subsequent purification of proteins necessary. The purification of proteins based on their precipitation or the use of ultrafiltration has been the usual procedure, but new strategies based on nanomaterials are also being explored. The release of potential antioxidant peptides from proteins is the next step. Microbial fermentation and, especially, digestion with enzymes such as Alcalase, thermolysin, or flavourzyme have been the most common. Released peptides are next characterized by the evaluation of their antioxidant properties and the application of proteomic tools to identify their sequences.

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Section: Obtaining Antioxidant Proteins and Peptides From Fruit Residuesmentioning

confidence: 99%

“…The extraction of proteins from pumpkin (Cucurbita moschata) seeds was performed using MAE and different DES as extracting solvents [64]. Poly (ethylene glycol) (PEG 200) was employed as the HBD, while several HBAs were tried.…”

Section: Microwave-assisted Extractionmentioning

confidence: 99%

Extraction and Characterization of Antioxidant Peptides from Fruit Residues

Olivares-Galván

Marina

García

2020

Foods

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“…In addition, the hydrolysates obtained from proteins extracted with the DES presented high antihypertensive capacity, which can be attributed to higher peptide release from the DES extract. On the other hand, Liu, Yu, Ge, Bai, Li, and Fu [ 96 ] employed microwave irradiation to assist DES-extraction of proteins from pumpkin seeds. A total of 93.95% of yield ( w/w ) was obtained and the protein precipitation rate was 97.97% by using an isoelectric point-ethanol-PEG 200 DES co-precipitation method.…”

Section: Des Extraction Of Biological Macromoleculesmentioning

confidence: 99%

Deep Eutectic Solvent as Green Solvent in Extraction of Biological Macromolecules: A Review

Ling

Hadinoto

2022

IJMS

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Greater awareness of environmental sustainability has driven many industries to transition from using synthetic organic solvents to greener solvents in their manufacturing. Deep eutectic solvents (DESs) have emerged as a highly promising category of green solvents with well-demonstrated and wide-ranging applications, including their use as a solvent in extraction of small-molecule bioactive compounds for food and pharmaceutical applications. The use of DES as an extraction solvent of biological macromolecules, on the other hand, has not been as extensively studied. Thereby, the feasibility of employing DES for biomacromolecule extraction has not been well elucidated. To bridge this gap, this review provides an overview of DES with an emphasis on its unique physicochemical properties that make it an attractive green solvent (e.g., non-toxicity, biodegradability, ease of preparation, renewable, tailorable properties). Recent advances in DES extraction of three classes of biomacromolecules—i.e., proteins, carbohydrates, and lipids—were discussed and future research needs were identified. The importance of DES’s properties—particularly its viscosity, polarity, molar ratio of DES components, and water addition—on the DES extraction’s performance were discussed. Not unlike the findings from DES extraction of bioactive small molecules, DES extraction of biomacromolecules was concluded to be generally superior to extraction using synthetic organic solvents.

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“…Extraction of protein from agri-food residues, such as wheat bran, rice bran, brewer’s spent grains, pomegranate seeds, cauliflower leaves, pumpkin seed, and coffee beans has been widely studied ( Liu et al, 2016 ; Phongthai et al, 2016 ; Xu et al, 2017 ; Talekar et al, 2018 ; Wen et al, 2020 ). During extraction, acid and alkali can hydrolyze proteins into smaller proteins or shorter peptides, which result in lower molecular weight of proteins in the extracts.…”

Section: Protein-rich Byproducts From Agri- and Aqua-culture Sidestreamsmentioning

confidence: 99%

Metabolic Engineering for Valorization of Agri- and Aqua-Culture Sidestreams for Production of Nitrogenous Compounds by Corynebacterium glutamicum

2022

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Establishment of an Aqueous PEG 200-Based Deep Eutectic Solvent Extraction and Enrichment Method for Pumpkin (Cucurbita moschata) Seed Protein

Cited by 59 publications

References 38 publications

Extraction and Characterization of Antioxidant Peptides from Fruit Residues

Extraction and Characterization of Antioxidant Peptides from Fruit Residues

Deep Eutectic Solvent as Green Solvent in Extraction of Biological Macromolecules: A Review

Metabolic Engineering for Valorization of Agri- and Aqua-Culture Sidestreams for Production of Nitrogenous Compounds by Corynebacterium glutamicum

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