Establishment of callogenesis and plant regeneration protocols for endemic Origanum syriacum ssp. sinaicum

El-Motaleb, M. A.; El-Hameid, Asmaa R. Abd; Helmy, Wafaa A.; Ewais, Emad A.; Abd-Elhady, M.S.

doi:10.1007/s12892-023-00198-0

Cited by 1 publication

(2 citation statements)

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“…These results agreed with [1,13], were best results for the surface sterilization of Stevia explants at low concentration of NaOCl. On the other hand, disagree with [19,20], who found least contamination and highest survival rate of stevia explants at 5% and 10% NaOCl respectively. Plant growth regulators are the basic factors for the success of establishing in vitro growth culture because of their important role on various responses e.g.…”

Section: Discussionmentioning

confidence: 71%

“…The highest rooted % shootlets 93% was recoded at 2.0 mg.l -1 IBA as compare with the lowest percentage of rooting 20% was obtained on medium on MS basal salt medium without hormones (control). These results are disagree with) [26], who recorded 30% of rooting on MS medium supplemented with 2.0 mg. l -1 IBA also disagree with [20], who found highest rooting 100% 99% at 0.5 mg. l -1 IBA followed by MS basal salt medium without hormones compare to 30% at 2.0 mg. l -1 IBA. Also data in the same table showed that 2.0 mg. l -1 IBA recorded highest number of roots.…”

Section: Root Induction and Plant Acclimatizationmentioning

confidence: 73%

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Employment of In Vitro Technique to Propagate Stevia (Stevia rebaudiana) Plant

Alani,

Elkaaby,

Alani

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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The current study was conducted with aim to find an efficient simple protocol for propagation of stevia plant ( Stevia rebaudiana ) In Vitro. Shoot tips, leaves and stem nodes explants were sterilized with different concentrations of NaOCl (0.0, 2.0, 4.0 and 6% v/v) then cultured on MS medium supplemented with 30 g.l-1 sucrose. Results revealed that, no contamination was recorded when using 4.0 % and 6.0 % of NaOCl with (100% and 8.9%) survival at 4.0% and 6.0 % respectively. Moreover, combination of plant growth regulators which were used in this study, have been successfully optimized. However, best combination for number of shoots. explant-1 was found to be (0.4 BAP + 0.2 Kin mg.l-1) for shoot tips explants with an average of 16.44 while (2.0 BAP + 0.25 GA3 mg. l-1) produced maximum average of plant height from shoot tips reached 4.85 cm. For in vitro roots induction, maximum root induction, number and length of roots. Shootlets reached (93.3 %, 9.77 and 9.0 cm) respectively when cultured shootlets on MS medium supplemented with 2.0 mg.l-1 of IBA.

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Section: Discussionmentioning

confidence: 71%

Section: Root Induction and Plant Acclimatizationmentioning

confidence: 73%