Establishment of canine models of lunatomalacia through liquid nitrogen freezing

Abstract

The aim of this study was to investigate the feasibility of establishing dog models of lunatomalacia through liquid nitrogen freezing. Twelve adult crossbred dogs were divided into three groups. Unilateral lunates were peeled off the parenchyma and frozen to result in avascular necrosis. They were observed dynamically through X-ray, computed tomography (CT) and magnetic resonance imaging (MRI). Furthermore, gross and histomorphological observations of samples were performed. Disseminated punctate hyperintense images and abnormal manifestations were detected, respectively. At 12 weeks after surgery, uneven bone density of the lunate and a flattened lunate of irregular shape were detected. A large area of irregular hypointense foci and hyperintensity was observed. Gross sample observation revealed a large area of dead bone. A decrease in the density of the trabecular bones and several vacant bone lacunas were visible. Liquid nitrogen freezing is a successful and reliable method for preparing animal models of lunatomalacia.

Adenovirus-Mediated Expression of BMP-2 and BFGF in Bone Marrow Mesenchymal Stem Cells Combined with Demineralized Bone Matrix For Repair of Femoral Head Osteonecrosis in Beagle Dogs

“…These features occur when blood flow is reduced by a mere 20% or more compared with that found in healthy people [2]. Bone repair is closely connected with the presence of blood vessels in the bone, and bone formation is initiated and supported by these vessels during bone growth [36]. Osteonecrosis is an orthopedic disease caused by diminished blood supply to the bone and will ultimately result in the death of osteocytes and BMCs [37].…”

Magnesium alloy transfected BMSCs-BMP-2 composite in repair of femoral head necrosis with assessment of visceral organs

“…Because the results of hip arthroplasty in patients with osteonecrosis are relatively poor, much focus has been on modalities aimed at femoral head preservation (Xiaobing et al 2015 ). Thus, to succeed in establishing that, the liquid nitrogen technique, easier and convenient, was used in this study because it achieves a high success rate with reliable osteonecrosis and a short pathological process and the basic properties of the bone are maintained (Berglund et al 2016 ; Huang et al 2013 ). In this study, the ONFH was properly established with the liquid nitrogen technique used, leading to a collapse of the femoral head in the control group and restoration of the femoral with perfect osteoids formation in the treatment group.…”

“…Osteoblasts differentiate and proliferate around the vessel, arrange along the vascular endothelium and then excrete osteoids in a direction away from the vessel. When the osteoblasts mature and develop into osteocytes, the newly formed bones deposit around the vessel (Huang et al 2013 ). Bone is a complex tissue that continually undergoes dynamic biological remodelling, i.e.…”