Establishment of cell lines that exhibit pluripotency from miniature swine periodontal ligaments

Ibi, Miho; Ishisaki, Akira; Yamamoto, Masahide; Wada, Satoshi; Kozakai, Takaharu; Nakashima, Aiko; Iida, Jynichiro; Takao, Sonshin; Izumi, Yuichi; Yokoyama, Atsuro; Tamura, Masato

doi:10.1016/j.archoralbio.2007.04.009

Cited by 25 publications

(23 citation statements)

References 31 publications

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“…It has been demonstrated that many genes trigger oncogene-induced senescence in vitro and in vivo [49]. Periostin has been shown to be highly expressed in immortalized human microvascular endothelial cells [50] and TesPDL cells, derived from miniature swine periodontal ligaments [51], where these cells are transfected with hTERT to prevent cell senescence. However, there are no reports on the direct role of periostin as an oncogene product that results in the unlimited replicative potential of cancer cells.…”

Section: Limitless Replicative Potentialmentioning

confidence: 99%

The multifaceted role of periostin in tumorigenesis

Ruan

Bao

Ouyang

2009

Cell. Mol. Life Sci.

293

299

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Periostin, also called osteoblast-specific factor 2 (OSF-2), is a member of the fasciclin family and a disulfide-linked cell adhesion protein that has been shown to be expressed preferentially in the periosteum and periodontal ligaments, where it acts as a critical regulator of bone and tooth formation and maintenance. Furthermore, periostin plays an important role in cardiac development. Recent clinical evidence has also revealed that periostin is involved in the development of various tumors, such as breast, lung, colon, pancreatic, and ovarian cancers. Periostin interacts with multiple cell-surface receptors, most notably integrins, and signals mainly via the PI3-K/Akt and other pathways to promote cancer cell survival, epithelial-mesenchymal transition (EMT), invasion, and metastasis. In this review, aspects related to the function of periostin in tumorigenesis are summarized.

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Section: Limitless Replicative Potentialmentioning

confidence: 99%

The multifaceted role of periostin in tumorigenesis

Ruan

Bao

Ouyang

2009

Cell. Mol. Life Sci.

293

299

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“…We previously showed that a swine PDL fibroblast cell line, namely, TesPDL3, could express EC markers and osteoblast markers in addition to PDL markers (9,10 that construct blood vessels with a mature lumen by evaluating their ability to vascularize in a 3-dimensional type I collagen scaffold. We established several single cell-derived cultures (SCDCs) from a primary culture of rat PDL fibroblasts.…”

Section: Introductionmentioning

confidence: 99%

Fibroblast growth factor-1-induced ERK1/2 signaling reciprocally regulates proliferation and smooth muscle cell differentiation of ligament-derived endothelial progenitor cell-like cells

Takahashi

Okubo²,

Chosa³

et al. 2011

Int J Mol Med

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Abstract. The periodontal ligament (PDL) is a fibrous connective tissue located between the tooth root and the alveolar bone. We previously demonstrated that a single cell-derived culture of primarily cultured PDL fibroblasts has the potential to construct an endothelial cell (EC) marker-positive blood vessel-like structure, suggesting that the fibroblastic lineage cells in ligament tissue could act as the endothelial progenitor cells (EPCs), which regenerate to construct a vascular system around the damaged ligament tissue. Moreover, we showed that EPC-like fibroblasts expressed not only EC markers but also smooth muscle cell (SMC) markers. Generally, an interaction between ECs and SMCs regulates blood vessel development and remodeling, and is required for the formation of a mature and functional vascular network. However, the mechanism underlying the SMC differentiation of the ligament-derived EPC-like fibroblasts remains to be clarified. In this study, we showed that suppression of fibroblast growth factor 1 (FGF-1)-induced extracellular signal-regulated kinase 1/2 (ERK1/2) signaling with the MAPK/ERK kinase (MEK) inhibitor U0126 completely abolished the FGF-1-induced proliferation of the ligament-derived EPC-like fibroblasts. In addition, U0126 treatment of FGF-1-stimulated ligamentderived EPC-like fibroblasts significantly induced the SMC differentiation of the cells. Thus, FGF-1-induced ERK1/2 signaling not only promoted the proliferation of the ligamentderived EPC-like fibroblasts, but also suppressed the SMC differentiation of the cells, suggesting that FGF-1 controls the construction of a vascular network around the ligament tissue by regulating the proliferation and SMC differentiation of the EPC-like cells through ERK-mediated signaling.

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“…2a). On the other hand, SCDCs consistently expressed mRNAs for extracellular matrix proteins of PDL, that is, α1(I) procollagen, OPN and periostin [15], although slight differences in their expression levels were observed (fig. 2b).…”

Section: Resultsmentioning

confidence: 99%

“…The PDL cell line, TesPDL, was established by over-expression of human telomerase reverse transcriptase (hTERT) in cells derived from swine PDL [15]. However, we cannot exclude the possibility that hTERT itself affects the expression of mesenchymal stem cell-like activity in cells derived from PDL.…”

Section: Introductionmentioning

confidence: 99%

Vascular Cell-Like Potential of Undifferentiated Ligament Fibroblasts to Construct Vascular Cell-Specific Marker-Positive Blood Vessel Structures in a PI3K Activation-Dependent Manner

Okubo¹,

Ishisaki²,

Iizuka

et al. 2010

J Vasc Res

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Objective: To evaluate whether fibroblasts derived from periodontal ligament retain the ability to differentiate into putative vascular cells and construct vascular cell-specific marker-positive blood vessel structures. We also evaluated the morphological features of the structure and investigated the intracellular molecular mechanism underlying the angiogenic activity of these cells. Methods: Single cell-derived cultures (SCDCs) were established from primary rat ligament fibroblast cultures, and their expression of ligament cell-, mesenchymal stem cell- and vascular cell-specific markers was evaluated by RT-PCR and immunocytochemistry. The ability of the cells to construct a blood vessel structure was evaluated in a three-dimensional type I collagen scaffold. The morphological and immunohistological characteristics of the structure were then evaluated. Results: Each SCDC expressed endothelial cell (EC)-specific and smooth muscle cell-specific markers, in addition to mesenchymal stem cell- and ligament cell-specific markers. SCDC2 cells, which abundantly expressed the EC markers Flk-1 and Tie-2, vigorously constructed a blood vessel structure in a phosphoinositide 3-kinase activation-dependent manner. Conclusion: Periodontal ligament fibroblasts have the potential to construct an EC marker-positive blood vessel-like structure. Consequently, the fibroblastic lineage in ligament tissue could be a candidate precursor for construction of a vascular system around damaged ligament tissue to facilitate its regeneration.

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Establishment of cell lines that exhibit pluripotency from miniature swine periodontal ligaments

Cited by 25 publications

References 31 publications

The multifaceted role of periostin in tumorigenesis

The multifaceted role of periostin in tumorigenesis

Fibroblast growth factor-1-induced ERK1/2 signaling reciprocally regulates proliferation and smooth muscle cell differentiation of ligament-derived endothelial progenitor cell-like cells

Vascular Cell-Like Potential of Undifferentiated Ligament Fibroblasts to Construct Vascular Cell-Specific Marker-Positive Blood Vessel Structures in a PI3K Activation-Dependent Manner

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