Establishment of efficient adventitious shoots induction system and ex vitro rooting in Vaccinium corymbosum (Ericaceae)

Abstract

<p align="left"><strong>Background</strong>: The extension of the blueberry industry in China is restricted by the low performance of proliferation coefficient, transplanting survival rate and the long cycle production.</p><p align="left"><strong>Hypothesis</strong>: We hypothesized the suitable medium with optimal concentration and type combination would improve the survival rate of <em>Vaccinium corymbosum.</em></p><p align="left"><strong>Species studied</strong>: <em>Vaccinium corymbosum</em> L. cultivar ‘O’Neal.</p><p align="left"><strong>Study site and years of study</strong>: Our study was conducted in Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese medicinal materials, Yunnan University of Chinese Medicine since November 2015.</p><p align="left"><strong>Methods</strong>: Efficient protocol of plant regeneration about Southern highbush blueberry (cultivar ‘O’Neal) was established using annual shoots via single factor and orthogonal experiments.</p><p align="left"><strong>Results</strong>: Olive medium with 2.0 mg·L<sup>-1</sup> zeatin was found to be most suitable for initiation culture. The highest callus induction and bud formation rate were determined with 93.67 % and 95.57 %, respectively. Furthermore, as the germination of axillary buds, numerous shoots were formed with the proliferation coefficient (> 60). Well-developed shoots were obtained using rejuvenation culture on half-strength Olive medium supplemented with combination of 2.0 mg·L<sup>-1</sup> indole-3-butyric acid, 1.0 mg·L<sup>-1</sup> naphthaleneacetic acid, 0.01 mg·L<sup>-1</sup> chlormequat chloride and 0.5 g·L<sup>-1</sup> activated charcoal. The rooting rate could reach to 100 % using 2 cm length of well-developed shoots transferred into the 5:1 sterilized peat:perlite, which was enhanced from 27.61 up to 95 % after transplanting to the field.</p><p align="left"><strong>Conclusions</strong>: An efficient system for plant regeneration from bud induction to transplanting to the field was established to maintain the characteristics of southern highbush blueberry varieties.</p>

Optimizing factors influencing micropropagation of ‘Bluecrop’ and ‘Biloxi’ blueberries and evaluation of their morpho-physiological characteristics during ex vitro acclimatization

“…On the other hand, leaves and stems of regenerated shoots in the control treatment (without AgNPs) had a yellowish-reddish coloration, an indicator of nutritional deficiency. The development of yellowish or reddish shoots was also reported by Wang et al [12] and Li et al [32], being a characteristic response of shoots grown in WPM due to its low nutrient concentration, particularly nitrogen. Nonetheless, the addition of AgNPs showed a possible hormetic effect on the shoots, as the vitroplants slightly improved their coloring.…”

“…For the initiation of in vitro propagation, the sterilization of plant material is of crucial importance, especially when the explants come from the field [9]. On the other hand, during the multiplication stage, different growth regulators are usually used to induce the development of new shoots [10][11][12]. However, in recent years, the addition of nanomaterials has been shown to be a revolutionary alternative to optimize organogenesis protocols [13,14].…”

In Vitro Propagation And Rejuvenation of Senescent Maternal Plant of Ardisia Crenata Var. Bicolor (Primulaceae)

“…Wang et al 27 reported that NAA has a weak in uence on buds, which suggested a signi cant role of optimal KT in increasing the proliferation rate. However, with increasing cytokinin concentrations negatively affected the shoot regeneration frequency, which was consistent with Rejthar et al 28 .…”

<i>In Vitro</i> Polyploid Induction and Establishment of a Clone for <i>Cyclocodon lancifolius</i> (Roxb.) Kurz

“…Plant tissue culture has been widely used in plant breeding, which has clearly advantages in maintaining clonal genetic characteristics, shortening the breeding cycle, improving reproductive efficiency and economic benefits (Wang et al 2019). Compared with the five stages for traditional tissue culture, including aseptic system establishment, primary culture, subculture and rapid propagation, rooting induction, and acclimatization, one-step culture is simpler and more efficient, and can avoid the germplasm variation (Loyola-Vargas and Ochoa-Alejo 2018).…”