Establishment of fin cell lines and their use to study the immune gene expression in cyprinid fishes with different ploidy in rhabdovirus infection

Xiao, Jun; Fu, Yukui; Zhou, Wei; Peng, Liangyue; Liu, Shaojun; Feng, Hao

doi:10.1016/j.dci.2018.07.007

Cited by 19 publications

(3 citation statements)

References 41 publications

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“…The pavement cells mainly cover the chloride ion cells on the branchial filaments and branchial lamella. The mucous cells are in the lamella epithelium and their secretion may be a mechanism to adapt to different water conditions [78]. A study identified the hypotonic responsive genes in gill cells and profiled the gill microbiota communities after a freshwater transfer experiment via transcriptome sequencing and 16S rRNA gene sequencing, suggesting the host-bacterium interaction in the gill facilitates freshwater acclimation [17].…”

Section: Fish Gill Cellsmentioning

confidence: 99%

“…The first advantage is the use of freshly isolated gill cells to obtain information on ion transport in filament surfaces and MRCs to explain the osmotic mechanism. The second advantage is that gill filaments can be cultured in a culture medium for almost four days [78]. The Percoll method is the best method for gill cell isolation; Percoll TM -gradient-isolated gill chloride (CC) and pavement cells (PVCs) were settled and the regulation of their expression in primary gill cell culture was determined [80][81][82].…”

Section: Fish Gill Cellsmentioning

confidence: 99%

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Profiling the Physiological Roles in Fish Primary Cell Culture

He,

Zhao,

Xiao

et al. 2023

Biology

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Fish primary cell culture has emerged as a valuable tool for investigating the physiological roles and responses of various cell types found in fish species. This review aims to provide an overview of the advancements and applications of fish primary cell culture techniques, focusing on the profiling of physiological roles exhibited by fish cells in vitro. Fish primary cell culture involves the isolation and cultivation of cells directly derived from fish tissues, maintaining their functional characteristics and enabling researchers to study their behavior and responses under controlled conditions. Over the years, significant progress has been made in optimizing the culture conditions, establishing standardized protocols, and improving the characterization techniques for fish primary cell cultures. The review highlights the diverse cell types that have been successfully cultured from different fish species, including gonad cells, pituitary cells, muscle cells, hepatocytes, kidney and immune cells, adipocyte cells and myeloid cells, brain cells, primary fin cells, gill cells, and other cells. Each cell type exhibits distinct physiological functions, contributing to vital processes such as metabolism, tissue regeneration, immune response, and toxin metabolism. Furthermore, this paper explores the pivotal role of fish primary cell culture in elucidating the mechanisms underlying various physiological processes. Researchers have utilized fish primary cell cultures to study the effects of environmental factors, toxins, pathogens, and pharmaceutical compounds on cellular functions, providing valuable insights into fish health, disease pathogenesis, and drug development. The paper also discusses the application of fish primary cell cultures in aquaculture research, particularly in investigating fish growth, nutrition, reproduction, and stress responses. By mimicking the in vivo conditions in vitro, primary cell culture has proven instrumental in identifying key factors influencing fish health and performance, thereby contributing to the development of sustainable aquaculture practices.

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Section: Fish Gill Cellsmentioning

confidence: 99%

Section: Fish Gill Cellsmentioning

confidence: 99%

Profiling the Physiological Roles in Fish Primary Cell Culture

He,

Zhao,

Xiao

et al. 2023

Biology

View full text Add to dashboard Cite

show abstract

“…Evidence in favor of the protective efficacy of the innate response has been found by analyzing the susceptibility to viral disease of selected populations of fish, where genetically resistant individuals seemed to exhibit a stronger innate immune response [ 135 , 136 , 137 ]. In some other instances, however, up-regulation of ifn , mx , and vig/viperin was associated with acute infection [ 55 ].…”

Section: Correlation Between Up-regulation Of Innate Immunity Genes A...mentioning

confidence: 99%

Fish Innate Immune Response to Viral Infection—An Overview of Five Major Antiviral Genes

Ortega-Villaizán¹,

Chico²,

Pérez³

2022

Viruses

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Fish viral diseases represent a constant threat to aquaculture production. Thus, a better understanding of the cellular mechanisms involved in establishing an antiviral state associated with protection against virus replication and pathogenesis is paramount for a sustainable aquaculture industry. This review summarizes the current state of knowledge on five selected host innate immune-related genes in response to the most relevant viral pathogens in fish farming. Viruses have been classified as ssRNA, dsRNA, and dsDNA according to their genomes, in order to shed light on what those viruses may share in common and what response may be virus-specific, both in vitro (cell culture) as well as in vivo. Special emphasis has been put on trying to identify markers of resistance to viral pathogenesis. That is, those genes more often associated with protection against viral disease, a key issue bearing in mind potential applications into the aquaculture industry.

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Diverse transcriptional patterns of homoeologous recombinant transcripts in triploid fish (Cyprinidae)

Ren

Zhang

et al. 2021

Sci. China Life Sci.

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Establishment of fin cell lines and their use to study the immune gene expression in cyprinid fishes with different ploidy in rhabdovirus infection

Cited by 19 publications

References 41 publications

Profiling the Physiological Roles in Fish Primary Cell Culture

Profiling the Physiological Roles in Fish Primary Cell Culture

Fish Innate Immune Response to Viral Infection—An Overview of Five Major Antiviral Genes

Diverse transcriptional patterns of homoeologous recombinant transcripts in triploid fish (Cyprinidae)

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