Establishment of GC-MS Fingerprint of Fresh Houttuynia cordata

Meng, Jianxin; Leung, Kelvin Sze-Yin; Jiang, Zhihong; Dong, Xiaoping; Zhao, Zhongzhen

doi:10.1248/cpb.53.1484

Cited by 32 publications

(27 citation statements)

References 13 publications

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“…25) However, the analysis in previously established GC-MS fingerprint only focused on those volatile components. These volatile compounds are always unstable and easily decomposed during the course of sample preparation and analysis.…”

Section: Hplc-ms Analysis For Components Identificationmentioning

confidence: 99%

“…[20][21][22][23][24] Recently, a method using GC-MS fingerprinting for quality evaluation was established by our research group. 25) In that part of work, GC-MS was employed to analyze for the volatile components in H. cordata but not yet covered with other phenolic and alkaloid-typed components. In fact, there still exists an analytical void for simultaneous detection of alkaloids, phenols and other major chemical compounds in H. cordata for comprehensive quality evaluation purpose.…”

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confidence: 99%

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Establishment of HPLC-DAD-MS Fingerprint of Fresh Houttuynia cordata

Meng

Leung

Jiang

et al. 2005

CHEMICAL & PHARMACEUTICAL BULLETIN

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Chromatographic fingerprinting technique is a useful and facile tool for the quality evaluation of herbal medicine and their related products. This technique has been introduced and accepted by WHO as a strategy for the quality assessment of herbal medicines in early 1990s.1) Among those commonly used methods, HPLC-DAD-MS technique has been used widely for the simultaneous qualitative and quantitative analysis of herbal medicine and their related products, with on-line UV and mass spectrometric information available for each individual peak in the chromatogram. In this regard, HPLC-DAD-MS fingerprint analysis has recently been considered as a more comprehensive and powerful technique for establishment of chromatographic fingerprinting. 2-4)Fresh Houttuynia cordata THUNB. has been a well known traditional Chinese medicinal material widely used in China, Japan and also listed in the Chinese Pharmacopoeia (2005 edition).5) It possesses a variety of pharmacological activities including anti-platelet aggregation, antibacterial, antitumor, antimicrobial, anti-inflammatory, antileukemic, immunomodulatory and recently, demonstrated its efficacy in anti-SARS. [6][7][8][9][10] In general, the chemical components of H. cordata comprised of three major types, namely: essential oil, phenols and alkaloid components.11) However, most of the previous related studies were mainly focused on the chemistry of essential oil, which has been considered responsible for the claimed clinical efficacy.12) Recent pharmacological studies also revealed that the flavonoid components in H. cordata possess antineoplastic, antioxidant, antimutagenic and free radical scavenging capacity. [13][14][15] Similarly, the alkaloid components demonstrated significant potent antiplatelet and cytotoxic activities, 7,16) whereas chlorogenic acid had significant antipyretic and antibiotic properties.17) Although a variety of pharmacological activities associated with these groups of chemical in H. cordata was demonstrated, the common used quality standard of H. cordata is still relied merely on the content of undecanone which served as a marker compound for the quality evaluation standard as stipulated in the Chinese Pharmacopoeia (2005 edition). 5)This phenomenon stimulated our thought to develop a chromatographic fingerprinting technique to associate various pharmacological activities with the chemical profile and thus the quality of H. cordata and its related products. Moreover, as the crude herbs are generally cultivated for use over China, a vast variety of external factors can therefore affect the chemical composition, and thus the pharmacological activity of that particular herbal medicine. These factors include geographical origin for cultivation, drying methods used, different medicinal portions, etc. [18][19] In this regard, a facile and comprehensive means for evaluating the overall quality of H. cordata is urgently needed.In literature, related analyses on H. cordata species are particularly focused on TLC, GC, GC-MS and a few of them on HPLC. [2...

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Section: Hplc-ms Analysis For Components Identificationmentioning

confidence: 99%

mentioning

confidence: 99%

Establishment of HPLC-DAD-MS Fingerprint of Fresh Houttuynia cordata

Meng

Leung

Jiang

et al. 2005

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…Several works using the CFs obtained by HPLC or GC for quality control of HMs have been reported with the help of similarity analysis and chemical pattern recognition [42][43][44][45][46][47][48][49][50].…”

Section: Quality Control Of Tcm Based On Pattern Analysis Of Cfsmentioning

confidence: 99%

“…is a commonly used Chinese materia medica in either fresh herb or sun-dried one. A comparative study by a modified GC-MS method coupled with pattern recognition could effectively distinguish the fresh herbs from the dry ones, even differentiate stems and leaves [43]. A HPLC chromatographic pattern matching successfully described the difference of whole chromatograms of raw and steamed roots of Panax notoginseng quantitatively [44].…”

Section: Quality Control Of Tcm Based On Pattern Analysis Of Cfsmentioning

confidence: 99%

Chromatographic fingerprinting and related chemometric techniques for quality control of traditional Chinese medicines

Liang

Xie²,

Chau

2010

J of Separation Science

122

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Development of chromatographic fingerprint (CF) and related chemometric methods and their applications to quality control of traditional Chinese medicines (TCMs) were discussed. CF is essentially a kind of quality control method for TCMs (or Chinese herbal medicines). Also, it is a quality-relevant-data high-throughput and integral tool to explore chemically the complexity of TCMs. With the help of chemometrics, some difficulties in evaluation and analysis of CFs, such as calculation of information content, peak alignment, pattern analysis, deconvolution of overlapping peaks, etc. could be well solved. To further explore TCMs synergic quality, intensive study of CF coupled with chemometrics will create the possibility to achieve the aim to reveal the working mechanisms of TCMs and to further control and strengthen TCMs' intrinsic quality in a comprehensive manner.

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“…A modified GC-MS method was developed in the comparison of fingerprints among fresh and dry herbs of H. cordata. The results showed that the developed fingerprint could distinguish between fresh and dry herbs, as well as determining differentiation among different medicinal portions [283]. Lu et al successfully developed a HPLC fingerprint method containing the isolation and identification of not only phthalides, but also organic acids and their esters by LC-DAD-MS simultaneously.…”

Section: Chromatographic Fingerprints Of Tcms and Chemometricsmentioning

confidence: 99%

Traditional Chinese medicine and separation science

Shao

Liang³

2008

J of Separation Science

124

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Traditional Chinese medicines (TCMs) are getting more and more popular nowadays in the whole world for improving health condition of human beings as well as preventing and healing diseases. TCM is a multi-component system with components mostly unknown, and only a few compounds are responsible for the pharmaceutical and/or toxic effects. The large numbers of other components in the TCM make the screening and analysis of the bioactive components extremely difficult. So, separation and analysis of the desired chemical components in TCM are very important subjects for modernization research of TCM. Thus, many novel separation techniques with significant advantages over conventional methods were introduced and applied to separation and analysis of the chemical constituents in TCM. This review presents just a brief outline of the applications of different separation methods for the isolation and analysis of TCM constituents.

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Establishment of GC-MS Fingerprint of Fresh Houttuynia cordata

Cited by 32 publications

References 13 publications

Establishment of HPLC-DAD-MS Fingerprint of Fresh Houttuynia cordata

Establishment of HPLC-DAD-MS Fingerprint of Fresh Houttuynia cordata

Chromatographic fingerprinting and related chemometric techniques for quality control of traditional Chinese medicines

Traditional Chinese medicine and separation science

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