Establishment of Hertwig’s epithelial root sheath cell line from cells involved in epithelial–mesenchymal transition

Akimoto, Tadashi; Fujiwara, Naoki; Kagiya, Tadayoshi; Otsu, Keishi; Ishizeki, Kiyoto; Harada, Hidemitsu

doi:10.1016/j.bbrc.2010.11.112

Cited by 48 publications

(48 citation statements)

References 14 publications

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“…In addition, cultured epithelial sheath cells show mesenchymal characteristics under given conditions (Thomas 1995;Zeichner-David et al 2003;Sonoyama et al 2007;Akimoto et al 2011). These findings have lead many investigators to assume that epithelial sheath cells transdifferentiate into cementoblasts through EMT during in vivo regular cementogenesis (Thomas 1995;Webb et al 1996;Bosshardt andNanci 1998, 2004;Lésot et al 2000;Zeichner-David et al 2003;Bosshardt 2005;Sonoyama et al 2007;Akimoto et al 2011). Some of these investigators emphasize that cementoblasts of epithelial origin are associated with only acellular cementogenesis, but not with cellular cementogenesis (Webb et al 1996;Zeichner-David et al 2003), and the others mention that cementoblasts of epithelial origin are associated with both acellular and cellular cementogenesis, or do not distinguish between two types of cementogenesis.…”

Section: Fate Of Epithelial Sheath Cellsmentioning

confidence: 99%

“…Many reports have emphasized that some epithelial sheath cells transform into cementoblasts through epithelialmesenchymal transition, or EMT (Thomas 1995;Webb et al 1996;Bosshardt andNanci 1998, 2004;Lésot et al 2000;Zeichner-David et al 2003;Bosshardt 2005;Huang et al 2009;Akimoto et al 2011). This novel idea can explain why the number of epithelial cells decreases immediately after epithelial sheath fragmentation, even though only a few epithelial cells die by apoptosis (Kaneko et al 1999;Suzuki et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Hertwig’s epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars

Yamamoto

Yamada

et al. 2014

Histochem Cell Biol

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This study was designed to examine developing acellular cementum in rat molars by immunohistochemistry, to elucidate (1) how Hertwig's epithelial root sheath disintegrates and (2) whether epithelial sheath cells transform into cementoblasts through epithelial-mesenchymal transition (EMT).Initial acellular cementogenesis was divided into three developmental stages, which can be seen in three different portions of the root: portion 1, where the epithelial sheath is intact; portion 2, where the epithelial sheath becomes fragmented; and portion 3, where acellular cementogenesis begins. Antibodies against three kinds of matrix proteinases, which degrade epithelial sheath-maintaining factors, including basement membrane and desmosomes, were used to investigate proteolytic activity of the epithelial sheath. Tissue non-specific alkaline phosphatase (TNALP) and keratin were used to investigate EMT. Epithelial sheath cells showed immunoreactivity for all three enzymes at fragmentation, which suggests that epithelial sheath disintegration is enzymatically mediated. Dental follicle cells and cementoblasts showed intense immunoreactivity for TNALP, and from portion 1 through to 3, the reaction extended from the alveolar bone-related zone to the root-related zone. Cells possessing keratin/TNALP double immunoreactivity were virtually absent. Keratin-positive epithelial sheath cells showed negligible immunoreactivity for TNALP, and epithelial cells did not appear to migrate to the dental follicle. Together, these findings suggest that a transition phenotype between epithelial cells and cementoblasts does not exist in the developing dental follicle, and hence that epithelial sheath cells do not undergo EMT during initial acellular cementogenesis.In brief, this study supports the notion that cementoblasts derive from the dental follicle. words

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Section: Fate Of Epithelial Sheath Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hertwig’s epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars

Yamamoto

Yamada

et al. 2014

Histochem Cell Biol

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“…There has been controversy over the EMT of HERS/ERM cells. Because HERS/ERM cells represent a heterogeneous population, not all HERS/ERM cells have the ability to undergo EMT (Akimoto et al, 2011;Zeichner-David et al, 2003). Recently, EMT was demonstrated to be one mechanism by which HERS/ERM cells generate cementum (Sonoyama et al, 2007;Xiong et al, 2012).…”

Section: Stemness In Immortalized Hers/erm Cellsmentioning

confidence: 99%

Establishment of Hertwig’s Epithelial Root Sheath/Epithelial Rests of Malassez Cell Line from Human Periodontium

Nam¹,

Kim²,

Kim³

et al. 2014

Molecules and Cells

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“…It has been suggested that some HERS cells undergo EMT in vivo (23,24) and that isolated root epithelial cells can differentiate into cementoblasts via EMT in vitro or ex vivo (23). Here we present the first in vivo evidence that some crown epithelia underwent EMT and differentiated into root lineage and cementoblast-like cells when epithelial Bmp signaling was blocked by the depletion of Bmpr1a.…”

Section: Discussionmentioning

confidence: 67%

“…1C and D). In both human and mouse teeth, some cells in the intact HERS coexpress epithelial markers and mesenchymal markers such as N-cadherin (N-cad) and vimentin (Vim) (23,24), which suggest the possibility of epithelial-mesenchymal transition (EMT) in HERS during root development. In AKO teeth, we noticed that many Krt14 ϩ dental epithelial cells on the molar crowns and at the labial side of incisors invaginated toward or into the dental pulps (Fig.…”

Section: Resultsmentioning

confidence: 99%

Cessation of Epithelial Bmp Signaling Switches the Differentiation of Crown Epithelia to the Root Lineage in a β-Catenin-Dependent Manner

Yang

Bai

Qin

et al. 2013

Molecular and Cellular Biology

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The differentiation of dental epithelia into enamel-producing ameloblasts or the root epithelial lineage compartmentalizes teeth into crowns and roots. Bmp signaling has been linked to enamel formation, but its role in root epithelial lineage differentiation is unclear. Here we show that cessation of epithelial Bmp signaling by Bmpr1a depletion during the differentiation stage switched differentiation of crown epithelia into the root lineage and led to formation of ectopic cementum-like structures. This phenotype is related to the upregulation of Wnt/␤-catenin signaling and epithelial-mesenchymal transition (EMT). Although epithelial ␤-catenin depletion during the differentiation stage also led to variable enamel defect and precocious/ectopic formation of fragmented root epithelia in some teeth, it did not cause ectopic cementogenesis and inhibited EMT in cultured dental epithelia. Concomitant epithelial ␤-catenin depletion rescued EMT and ectopic cementogenesis caused by Bmpr1a depletion. These data suggested that Bmp and Wnt/␤-catenin pathways interact antagonistically in dental epithelia to regulate the root lineage differentiation and EMT. These findings will aid in the design of new strategies to promote functional differentiation in the regeneration and tissue engineering of teeth and will provide new insights into the dynamic interactions between the Bmp and Wnt/␤-catenin pathways during cell fate decisions.

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Establishment of Hertwig’s epithelial root sheath cell line from cells involved in epithelial–mesenchymal transition

Cited by 48 publications

References 14 publications

Hertwig’s epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars

Hertwig’s epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars

Establishment of Hertwig’s Epithelial Root Sheath/Epithelial Rests of Malassez Cell Line from Human Periodontium

Cessation of Epithelial Bmp Signaling Switches the Differentiation of Crown Epithelia to the Root Lineage in a β-Catenin-Dependent Manner

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