Establishment of Mycobacterium avium subsp. paratuberculosis infection in the intestine of ruminants

Sigurðardóttir, Ólöf; Valheim, Mette; Press, Charles McL.

doi:10.1016/j.addr.2003.10.032

Cited by 52 publications

(29 citation statements)

References 92 publications

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“…paratuberculosis with ileal tissue in cattle and goats, using segmented ileal loop and everted ileum models, has shown that M. avium subsp. paratuberculosis is rapidly taken up within minutes by microfold cells, enterocytes, macrophages, and most likely dendritic cells (19,28,(37)(38)(39)44). Examination of mesenteric lymph nodes and other peripheral tissue following direct inoculation into the ileum, using a surgical intervention model, revealed that M. avium subsp.…”

Section: Discussionmentioning

confidence: 99%

Development of a Bovine Ileal Cannulation Model To Study the Immune Response and Mechanisms of Pathogenesis of Paratuberculosis

Allen

Park

Barrington

et al. 2009

Clin Vaccine Immunol

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Section: Discussionmentioning

confidence: 99%

Development of a Bovine Ileal Cannulation Model To Study the Immune Response and Mechanisms of Pathogenesis of Paratuberculosis

Allen

Park

Barrington

et al. 2009

Clin Vaccine Immunol

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“…these lesions result in a thickness of the intestinal wall, resulting in decreased absorption, and fluid secretion into the gut lumen. lesions are largely restricted to the ileum, or the ileocecal valve region of the small intestine (Wu et al, 2007;coussens, 2004;sigurethardottir et al, 2004;stabel, 2000).…”

mentioning

confidence: 99%

Mycobacterium avium subsp. paratuberculosis and Crohn's Disease

Naser

Naser²

2014

Emerging Infections 7

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“…The primary site of M. avium subsp. paratuberculosis invasion is thought to be the terminal small intestine or ileum, with bacterial uptake by M cells in the Peyer's patches (PP) (8) or direct infection of absorptive epithelium (9,10). Once M. avium subsp.…”

mentioning

confidence: 99%

Host Responses to Persistent Mycobacterium avium Subspecies paratuberculosis Infection in Surgically Isolated Bovine Ileal Segments

Charavaryamath

González-Cano

Fries

et al. 2013

Clin Vaccine Immunol

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A lack of appropriate disease models has limited our understanding of the pathogenesis of persistent enteric infections with Mycobacterium avium subsp. paratuberculosis. A model was developed for the controlled delivery of a defined dose of M. avium subsp. paratuberculosis to surgically isolated ileal segments in newborn calves. The stable intestinal segments enabled the characterization of host responses to persistent M. avium subsp. paratuberculosis infections after a 9-month period, including an analysis of local mucosal immune responses relative to an adjacent uninfected intestinal compartment. M. avium subsp. paratuberculosis remained localized at the initial site of intestinal infection and was not detected by PCR in the mesenteric lymph node. M. avium subsp. paratuberculosis-specific T cell proliferative responses included both CD4 and ␥␦ T cell receptor (␥␦TcR) T cell responses in the draining mesenteric lymph node. The levels of CD8 ؉ and ␥␦TcR ؉ T cells increased significantly (P < 0.05) in the lamina propria, and M. avium subsp. paratuberculosis-specific tumor necrosis factor alpha (TNF-␣) and gamma interferon secretion by lamina propria leukocytes was also significantly (P < 0.05) increased. There was a significant (P < 0.05) accumulation of macrophages and dendritic cells (DCs) in the lamina propria, but the expression of mucosal toll-like receptors 1 through 10 was not significantly changed by M. avium subsp. paratuberculosis infection. In conclusion, surgically isolated ileal segments provided a model system for the establishment of a persistent and localized enteric M. avium subsp. paratuberculosis infection in cattle and facilitated the analysis of M. avium subsp. paratuberculosis-specific changes in mucosal leukocyte phenotype and function. The accumulation of DC subpopulations in the lamina propria suggests that further investigation of mucosal DCs may provide insight into host responses to M. avium subsp. paratuberculosis infection and improve vaccine strategies to prevent M. avium subsp. paratuberculosis infection.

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Establishment of Mycobacterium avium subsp. paratuberculosis infection in the intestine of ruminants

Cited by 52 publications

References 92 publications

Development of a Bovine Ileal Cannulation Model To Study the Immune Response and Mechanisms of Pathogenesis of Paratuberculosis

Development of a Bovine Ileal Cannulation Model To Study the Immune Response and Mechanisms of Pathogenesis of Paratuberculosis

Mycobacterium avium subsp. paratuberculosis and Crohn's Disease

Host Responses to Persistent Mycobacterium avium Subspecies paratuberculosis Infection in Surgically Isolated Bovine Ileal Segments

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