Establishment of nude mice model of human osteosarcoma cell MG63 with different potential of metastasis

Abstract

Objective: To establish a nude mice model of human osteosarcoma lung metastasis. Methods: The growth of human osteosarcoma cell sublines M8 and M6 was determined by MTT assay. 2 × 10 7 cells were injected into the tail vein of nude mice. Mice were sacrificed started on week 4 after injection, and lung metastases were evaluated under both macroscopic and microscopic observation with HE staining. Results: The growth of low-metastatic subline M6 was lower than high-metastatic sublines M8. Seventeen mice after injected M8 had occurred lung metastases while only one mice had occurred in M6 group. Moreover, M8 cells within metastases were arrangement disorder with variable nuclear hyperchromasia. Conclusion: A mouse model for human osteosarcoma cancer lung metastasis can be established by injection different ability of metastasis MG63 cells into tail vein.

Caffeic Acid Inhibits Tumour Mass Formation in MG-63 Cells-induced Nude Mice

“…Successful plantation of MG-63 cells in nude mice have been reported previously, which resulted in the establishment of metastasis mice models for osteosarcoma tumour types. (18,19) Tumour growth are also present after the injection of MG-63 tumour cell line to the subcutaneous space of shaved severe combined immunodeficiency (SCID) mice (18), which was similar to current study. Ninety days after the injection of MG-63 cells, tumour mass was observed in the subcutaneous area of nude mice, forming obvious nodules in the area that were not treated with caffeic acid.…”

Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis

“…This panel includes the parental Hu-09 cell line (included in Figure 6), and its highly metastatic derivatives M112 and M132 (derived by in vivo metastatic selection), as well as the poorly metastatic sub-clones L06 and L13 (all generously provided by Dr. B. Fuchs, the University of Zurich, Zurich, Switzerland) [106,107]. Additionally the poorly metastatic SAOS2 and MG63 cell lines, as well as their highly metastatic derivatives LM7 and M8, were included (generously provided by Dr. E. Kleinerman, University of Texas MD Anderson Cancer Center, Houston, TX, USA) [108,109]. In this panel, it was observed that the PRKCε-RASGRP3-GNB2 network exhibited an mRNA expression pattern similar to that observed in the expression profiles of osteosarcoma tumors (Figure 6).…”

“…The differences in metastatic propensity correlate to different survival lengths for mice injected with the various cell lines [107]. M8 cells were derived from MG63 human OS cells (hereafter referred to as MG63-P), and have decreased latency until pulmonary metastasis [109]. Both were grown in Dulbecco’s Modified Eagle Medium supplemented with 10% FBS.…”

Soft Tissue and Bone Sarcoma

“…This panel includes the parental Hu-09 cell line (included in Figure 6), and its highly metastatic derivatives M112 and M132 (derived by in vivo metastatic selection), as well as the poorly metastatic sub-clones L06 and L13 (all generously provided by Dr. B. Fuchs, the University of Zurich, Zurich, Switzerland) [106,107]. Additionally the poorly metastatic SAOS2 and MG63 cell lines, as well as their highly metastatic derivatives LM7 and M8, were included (generously provided by Dr. E. Kleinerman, University of Texas MD Anderson Cancer Center, Houston, TX, USA) [108,109]. In this panel, it was observed that the PRKC -RASGRP3-GNB2 network exhibited an mRNA expression pattern similar to that observed in the expression profiles of osteosarcoma tumors (Figure 6).…”

“…The differences in metastatic propensity correlate to di fferent survival lengths for mice injected with the various cell lines [107]. M8 cells were derived from MG63 human OS ce lls (hereafter referred to as MG63-P), and have decreased latency until pulmonary metastasis [109]. Both were grown in Dulbecco's Modified Eagle Medium supplemented with 10% FBS.…”