AIm: This study reports a technique for treating severe thoracolumbar fractures with single-stage decompression, reduction, reconstruction, and stabilization via an entirely posterior approach. mAterIAl and methOds:The cases of 11 patients with severe traumatic thoracolumbar fractures/dislocations that were managed with single-stage decompression, reduction, reconstruction, and stabilization via an entirely posterior approach were included. Data on age, sex, mechanism of injury, associated trauma, neurological status, surgical technique, and clinical outcome were reviewed retrospectively. results: Of the 11 patients, two presented with primarily coronal displacement and nine with sagittal displacement. Coronal displacement was corrected from 19% preoperatively to 4.0% at the last follow-up evaluation. Sagittal displacement was reduced from 73.0% preoperatively to 3.0% at the last follow-up evaluation. After a mean follow-up period of 20.7 (range 16-30) months, no patient complained of local pain and no significant loss of correction or hardware failure was observed.COnClusIOn: Our experience proves that the single-stage posterior approach is safe and biomechanically reliable for treating severe thoracolumbar fractures/dislocations. Therefore, the presented technique is an alternative approach for this rare injury.KeywOrds: Thoracolumbar fracture, Severe dislocation, Single-stage, Posterior approach ÖZ AmAÇ: Bu çalışma, tamamen posterior bir yaklaşımla tek evreli dekompresyon, redüksiyon, rekonstrüksiyon ve stabilizasyon ile şiddetli torakolumber kırıkları tedavi etmek için bir tekniği bildirmektedir. yÖntem ve GereÇler: Tamamen posterior yaklaşımla tek evreli dekompresyon, redüksiyon, rekonstrüksiyon ve stabilizasyonla tedavi edilen şiddetli travmatik torakolumber kırık/dislokasyonu olan 11 vaka dahil edilmiştir. Yaş, cinsiyet, yaralanma mekanizması, ilgili travma, nörolojik durum, cerrahi teknik ve klinik sonuçlarla ilgili veriler retrospektif olarak gözden geçirilmiştir.BulGulAr: 11 hasta içinde ikisi primer koronal displasman ve dokuzu sajital displasmanla gelmiştir. Sajital displasman preoperatif olarak %19'dan son takip değerlendirmesinde %4,0'a düzeltilmiştir. Sajital displasman preoperatif olarak %73,0'dan son takip değerlendirmesinde %3,0'a düzeltilmiştir. Ortalama 20,7 (aralık 16-30) aylık takip süresiyle hiçbir hasta yerel ağrıdan yakınmamış ve düzeltmede önemli bir kayıp veya donanımla ilgili başarısızlık gözlenmemiştir. sOnuÇ: Deneyimimiz tek evreli posterior yaklaşımın şiddetli torakolumber kırıklar/dislokasyonlar tedavisinde güvenli ve biyomekanik olarak uyumlu olduğunu göstermiştir. Bu nedenle sunulan teknik bu nadir yaralanma için alternatif bir yaklaşımdır.

Background: Cosmetic filler injection can cause a variety of eye complications; however, there is currently no good way to evaluate injury severity and prognosis. By analyzing the injury manifestations of severe ocular complications following cosmetic filler injection and their prognosis, we propose a new injury severity scale. Methods: Twenty-two eyes of 22 patients experiencing ocular complications following cosmetic filler injection were followed for 6 months to observe injury characteristics, manifestations and prognosis. Best corrected visual acuity (BCVA), intraocular pressure (IOP), split lamp microscopy, fundus photography, optical coherence tomography (OCT), and fundus fluorescein angiography were examined at the onset and follow-up visits. Results: According to the immediate BCVA at the time of injury (with the presence or absence of brain infarction), a new injury severity scale was proposed, namely, Grades 1-4. Grade 1 (4 patients) and Grade 2 (2 patients) tended to have no atrophy of the globe. Grade 3 (12 patients) and Grade 4 (4 patients) were more likely to develop atrophy of the globe (4/12 patients and 2/4 patients, respectively) at the last follow-up. Grade 3 and Grade 4 were more likely to be complicated with ophthalmoplegia and ptosis (7/16 patients). Conclusions: The new injury severity scale we proposed can determine the prognosis of different ocular complications following cosmetic filler injection. Accordingly, we can inform injured patients regarding the possibility of phthisis bulbi and the extent of improvement of visual impairment, ophthalmoplegia, ptosis and stroke.

A novel coronavirus (SARS-CoV-2) causing corona virus disease 2019 (COVID-19) has attracted global attention due to its highly infectious and pathogenic properties. Most of current studies focus on aerosols released from infected individuals, but the presence of SARS-CoV-2 in wastewater also should be examined. In this review, we used bibliometrics to statistically evaluate the importance of water-related issues in the context of COVID-19. The results show that the levels and transmission possibilities of SARS-CoV-2 in wastewater are the main concerns, followed by potential secondary pollution by the intensive use of disinfectants, sludge disposal, and the personal safety of workers. The presence of SARS-CoV-2 in wastewater requires more attention during the COVID-19 pandemic. Thus, the most effective techniques, i.e., wastewater-based epidemiology and quantitative microbial risk assessment, for virus surveillance in wastewater are systematically analyzed. We further explicitly review and analyze the successful operation of a sewage treatment plant in Huoshenshan Hospital in China as an example and reference for other sewage treatment systems to properly ensure discharge safety and tackle the COVID-19 pandemic. This review offers deeper insight into the prevention and control of SARS-CoV-2 and similar viruses in the post-COVID-19 era from a wastewater perspective.

Objective: To establish a nude mice model of human osteosarcoma lung metastasis. Methods: The growth of human osteosarcoma cell sublines M8 and M6 was determined by MTT assay. 2 × 10 7 cells were injected into the tail vein of nude mice. Mice were sacrificed started on week 4 after injection, and lung metastases were evaluated under both macroscopic and microscopic observation with HE staining. Results: The growth of low-metastatic subline M6 was lower than high-metastatic sublines M8. Seventeen mice after injected M8 had occurred lung metastases while only one mice had occurred in M6 group. Moreover, M8 cells within metastases were arrangement disorder with variable nuclear hyperchromasia. Conclusion: A mouse model for human osteosarcoma cancer lung metastasis can be established by injection different ability of metastasis MG63 cells into tail vein.