Establishment of Plantlets and Evaluation of Differentiated Roots for Alkaloids in Rauwolfia serpentha

Sehrawat, Anita R.; Uppal, S.; Chowdhury, J. B.

doi:10.1007/bf03263144

Cited by 7 publications

(2 citation statements)

References 5 publications

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“…The MS medium, without any growth hormones, was unable to induce callus. Among all the growth hormones, IAA and 2,4-D hormones exhibited more competence in callus induction in individual along with their different combinations which correlate the earlier reports [11,15]. The MS medium supplemented with different combination of enzyme, with different concentration varying from 0.5 to 5 mg/L of IAA, NAA, 2,4-D, BAP, and Kin exhibited stimulation and induction of callus.…”

Section: Effects Of Hormonal Treatments On Callus Inductionsupporting

confidence: 87%

Effect of growth hormones in induction of callus, antioxidants, and antibacterial activity in Nerium odorum

Avinash¹,

Umesha²

2018

J App Biol Biotech

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Nerium odorum is an imperative species with worldwide therapeutic and commercial uses. N. odorum has been considered as a potentially important plant for industrial and pharmacological applications. Callus induction potential of N. odorum was assessed from leaf explants cultured on Murashige and Skoog's medium using different plant hormonal treatments. A range of different concentrations of 6-benzylaminopurine, 1-naphthaleneacetic acid, 2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA), and kinetin (0.5-5.0 mg/L) have been incorporated in the culture medium to investigate the biomass, polyphenols production and oxidizing and antibacterial activity of the callus. The results showed highest callus induction at 2.0 mg/L in growth hormone combination of IAA and 2,4-D. The total phenolic content was 92.14 mg GA/g dry weight (DW). In addition, the 2,2-diphenyl-1-picrylhydrazyl activity and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) activities were 46.7 µg/mL DW and 19.9 µg/mLDW, respectively. Interestingly, the explants produced hard calli using combination of IAA and 2,4-D presented a higher phenolic content and antioxidant capacity when compared to individual growth hormones of either IAA or 2,4-D. Altogether, these results demonstrate the extraordinary effect of different growth hormones on polyphenols production, antioxidant, and antibacterial activities in N. odorum.

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Section: Effects Of Hormonal Treatments On Callus Inductionsupporting

confidence: 87%

Effect of growth hormones in induction of callus, antioxidants, and antibacterial activity in Nerium odorum

Avinash¹,

Umesha²

2018

J App Biol Biotech

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“…MS media fortified with 2, 4-D and BAP was found the best for callus induction [21]. Day of callus induction started from 17th to 37th day [22]. This variation observed in the present investigation may be attributed due to the difference in culture conditions and the age of explants.…”

Section: Callusingmentioning

confidence: 49%

Rapid In-Vitro Regeneration of an Important Medicinal and an Ornamental Plant (Catharanthus roseus L)

Rani¹

2015

Biochem Anal Biochem

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Catharanthus roseus (L.) is an important alkaloid-yielding medicinal and ornamental plant belonging to family Apocynaceae. It comprises of total 8 species. The genus Catharanthus is well reported for producing biologically active terpenoid indole alkaloids (TIAs) with over 130 compounds isolated and identified. This plant produces secondary metabolites called alkaloids which have a great medicinal importance such as circulatory ailments (antihypertensives), ajmalicine to treat diabetes, malaria, menorrhagia, Hodgkin's disease. This plant is grown commercially for its medicinal and ornamental uses in India. In view of these facts the study was conducted for micropropagation of Catharanthus roseus MS media supplemented with different concentrations (0.5-10.0 mg/l) of NAA, 2, 4-D, BAP and KIN were used singly and in combination. Among all the growth hormones 2, 4-D was the best for callus induction (85% in stem and 87% in leaf) and in combination 2, 4-D and BAP (65% in stem and 81% in leaf). Day of callus induction started from 13 th to 37 th day. This variation is due to the differences in culture conditions and the age of explants. In single combination BAP was the best for shooting (79%) and in double combinations BAP (1.5 mg/l) and NAA (0.5 mg/l) were the best for shooting (85%). Higher induction of root (91%) was observed at NAA (2 mg/l) and in double combinations BAP (1.5 mg/l) and NAA (0.5 mg/l) were the best for rooting (89%). Regenerated plants after hardenings were transferred to soil and they showed 77% survival. The protocol was optimized by manipulations of different plant hormones for enhanced multiplication. Protocol explained here provides a rapid plant regeneration system which could be used for the commercial purposes.

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Biotechnological Approaches Towards Improvement of Medicinal Plants

Narula

Kumar

Bansal

et al.

Plant Biotechnology and Molecular Markers

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Establishment of Plantlets and Evaluation of Differentiated Roots for Alkaloids in Rauwolfia serpentha

Cited by 7 publications

References 5 publications

Effect of growth hormones in induction of callus, antioxidants, and antibacterial activity in Nerium odorum

Effect of growth hormones in induction of callus, antioxidants, and antibacterial activity in Nerium odorum

Rapid In-Vitro Regeneration of an Important Medicinal and an Ornamental Plant (Catharanthus roseus L)

Biotechnological Approaches Towards Improvement of Medicinal Plants

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