Establishment of Rhodiola quadrifida Hairy Roots and Callus Culture to Produce Bioactive Compounds

Stepanova, A. Yu.; Malunova, Maria Victorovna; Salamaikina, Svetlana; Selimov, Renat N.; Solov'eva, A. I.

doi:10.32604/phyton.2021.013223

Cited by 9 publications

(8 citation statements)

References 21 publications

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“…The hairy roots of S. lateriflora , S. przewalskii and S. pycnoclada were obtained for this study. The important feature of the transformation method used in this study is the prolonged co-cultivation of explants in an agrobacterial suspension, which, as has been shown previously [ 37 ], allowed a higher percentage of transformed explants to be obtained ( Figure 1 ).…”

Section: Resultsmentioning

confidence: 74%

“…The A. rhizogenes suspension culture was grown in YEB liquid medium [ 48 ] for 24 h at 23 °C on a circular shaker (amplitude 5–10 cm, rotation speed 90 rpm) before the transformation of the plant material. The hairy roots of S. pycnoclada , S. lateriflora and S. przewalskii were obtained as described previously [ 37 ]. The explants (cotyledons and hypocotyls) were incubated in Agrobacterium suspension for 12 h, then they were transplanted into fresh B 5 medium with the addition of 500 mg/L of cefotaxime (Claforan, Great Britain).…”

Section: Methodsmentioning

confidence: 99%

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Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

et al. 2021

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We measured and studied the growth parameters and the qualitative and quantitative composition of the flavones of hairy roots of the Scutellaria genus: S. lateriflora, S. przewalskii and S. pycnoclada. Hairy roots were obtained using wild-type Agrobacterium rhizogenes A4 by co-cultivation of explants (cotyledons) in a suspension of Agrobacterium. The presence of the rol-genes was confirmed by PCR analysis. The hairy roots of the most studied plant from the Scutellaria genus, S. baicalensis, were obtained earlier and used as a reference sample. HPLC-MS showed the predominance of four main flavones (baicalin, baicalein, wogonin and wogonoside) in the methanol extracts of the studied hairy roots. In addition to the four main flavones, the other substances which are typical to the aerial part of plants were found in all the extracts: apigenin, apigetrin, scutellarin and chrysin-7-O-β-d-glucuronide. According to the total content of flavones, the hairy roots of the studied skullcaps form the following series: S. przewalskii (33 mg/g dry weight) > S. baicalensis (17.04 mg/g dry weight) > S. pycnoclada (12.9 mg/g dry weight) > S. lateriflora (4.57 mg/g dry weight). Therefore, the most promising producer of anti-coronavirus flavones is S. przewalskii.

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Section: Resultsmentioning

confidence: 74%

Section: Methodsmentioning

confidence: 99%

Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

et al. 2021

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“…Rhodiola quadrifida (Pall.) calluses are obtained from hairy roots in the MS medium with the addition of hormones: 3 mg/L 2,4-D and 0.5 mg/L BAP [ 31 ]. Calluses of R. sachalinensis Boriss.…”

Section: Discussionmentioning

confidence: 99%

“…Various concentrations and combinations of plant growth regulators have been used to obtain callus and suspension cultures of Rhodiola species. For the induction of callus formation in these species, a combination of cytokinins and auxins is required [ 10 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ]. 6-benzylaminopurine (BAP) is one of the most widely used plant growth regulators for in vitro culture of Rhodiola .…”

Section: Introductionmentioning

confidence: 99%

“…6-benzylaminopurine (BAP) is one of the most widely used plant growth regulators for in vitro culture of Rhodiola . Cotyledons, hypocotyl, leaves, apical buds, and internode fragments, inoculated on media containing BAP (0.2–3 mg/L) in combination with indole-3-acetic acid (IAA; 0.1 mg/L) [ 24 ], α-naphthylacetic acid (NAA; 0.5–3 mg/L) [ 10 , 28 ], or 2,4-dichlorophenoxyacetic acid (2,4-D; 0.5–3.0 mg/L) [ 25 , 31 ] have been the most appropriate explant types and media constituents for induction of well-growing calluses [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Biomass Accumulation and Production of Phenolic Compounds in Callus Cultures of Rhodiola rosea L. Using Design of Experiments

Эрст

Petruk

Erst

et al. 2022

Plants

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Rhodiola rosea L. is a valuable medicinal plant with adaptogenic, neuroprotective, antitumor, cardioprotective, and antidepressant effects. In this study, design of experiments methodology was employed to analyze and optimize the interacting effects of mineral compounds (concentration of NO3− and the ratio of NH4+ to K+) and two plant growth regulators [total 6-benzylaminopurine (BAP) and α-naphthylacetic acid (NAA) concentration and the ratio of BAP to NAA] on the growth and the production of total phenolic compounds (TPCs) in R. rosea calluses. The overall effect of the model was highly significant (p < 0.0001), indicating that NH4+, K+, NO3−, BAP, and NAA significantly affected growth. The best callus growth (703%) and the highest production of TPCs (75.17 mg/g) were achieved at an NH4+/K+ ratio of 0.33 and BAP/NAA of 0.33, provided that the concentration of plant growth regulators was 30 μM and that of NO3− was ≤40 mM. According to high-performance liquid chromatography analyses of aerial parts (leaves and stems), in vitro seedlings and callus cultures of R. rosea contain no detectable rosarin, rosavin, rosin, and cinnamyl alcohol. This is the first report on the creation of an experiment for the significant improvement of biomass accumulation and TPC production in callus cultures of R. rosea.

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Growth kinetics, metabolite yield, and expression analysis of biosynthetic pathway genes in friable callus cell lines of Rhodiola imbricata (Edgew)

Rattan

Kumar

Warghat

2021

Plant Cell Tiss Organ Cult

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Establishment of Rhodiola quadrifida Hairy Roots and Callus Culture to Produce Bioactive Compounds

Cited by 9 publications

References 21 publications

Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

Optimization of Biomass Accumulation and Production of Phenolic Compounds in Callus Cultures of Rhodiola rosea L. Using Design of Experiments

Growth kinetics, metabolite yield, and expression analysis of biosynthetic pathway genes in friable callus cell lines of Rhodiola imbricata (Edgew)

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