Establishment of stem cell lines from nuclear transferred and parthenogenetically activated mouse oocytes for therapeutic cloning

Ju, Jin Young; Park, Chun Young; Gupta, Mukesh Kumar; Uhm, Sang Jun; Paik, Eun Chan; Ryoo, Zae Young; Cho, Youl Hee; Chung, Kyuha; Lee, Hoon Taek

doi:10.1016/j.fertnstert.2006.11.203

Cited by 17 publications

(8 citation statements)

References 29 publications

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“…At present, mouse embryonic stem cell lines are sometimes established using parthenogenetic activation technology (8,9). However, since parthenogenetic mouse embryos rarely develop to the full term to be born, research data on parthenogenetic activation technology in mouse oocytes are extremely limited (5).…”

Section: Discussionmentioning

confidence: 99%

“…It has been suggested that rabbits are particularly suitable for use in studies of parthenogenetic activation technology (6). Although there have been studies where live embryos of apomixic mice were born and developed into mature individuals with reproductive capacity (7) and mouse embryonic stem cell lines have been established by parthenogenetic activation technology (8,9), parthenogenetic mouse embryos rarely develop to the full term to be born. Therefore, study data concerning parthenogenetic activation technology for mouse oocytes is extremely limited at present (5).…”

Section: Introductionmentioning

confidence: 99%

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Effects of chemical combinations on the parthenogenetic activation of mouse oocytes

Han¹,

Gao

2013

Experimental and Therapeutic Medicine

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The aim of this study was to identify an optimal method for the parthenogenetic activation of mouse oocytes. Ethanol (EH), strontium chloride (SrCl2) and ionomycin calcium salt were each combined with cytochalasin B to induce the parthenogenetic activation of CD-1® mouse oocytes. Among the EH combination groups, the blastocyst formation and hatching rates of the group that was activated with EH and CB for 5 min were significantly higher compared with those of the groups that were activated for 7 and 10 min (P<0.05). Among the SrCl2 combination groups, the blastocyst formation and hatching rates of the group that was activated with SrCl2 and CB for 30 min were significantly higher compared with those of the groups that were activated for 1 and 2 h (P<0.05). Among the ionomycin calcium salt combination groups, the blastocyst formation and hatching rates of the group that was activated with ionomycin and CB for 3 min were higher compared with those of the groups that were activated for 5 and 7 min (P<0.05). Compared with the other two combinations, the experimental indicators of the EH combination groups were notably superior (P<0.05). For combined activation, simultaneous activation with two substances was significantly more effective than successive activation (P<0.05). For combined activation with EH and cytochalasin B in mouse oocytes, 5 min of parthenogenetic activation had significant advantages with regard to cleavage, blastocyst formation and blastocyst hatching rates. In addition, the activation rate of combined activation was higher than that of single activators. For combined activation, the simultaneous application of two activators has a superior effect.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of chemical combinations on the parthenogenetic activation of mouse oocytes

Han¹,

Gao

2013

Experimental and Therapeutic Medicine

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“…Chromosome deletions and translocations have also been reported in the hPES-2 cell line, when cultivated for 120 passages [Mai et al, 2007]. Few cytogenetic data are available on mouse pESC lines, reporting a stable karyotype during culture (up to passage 120), analyzed by standard G-banding [Shao et al, 2007;Ju et al, 2008;Shan et al, 2012].…”

Section: Parthenogenetic Embryonic Stem Cellsmentioning

confidence: 99%

Chromosomal Abnormalities in Embryonic and Somatic Stem Cells

Rebuzzini¹,

Zuccotti

Redi³

et al. 2015

Cytogenet Genome Res

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The potential use of stem cells (SCs) for tissue engineering, regenerative medicine, disease modeling, toxicological studies, drug delivery, and as in vitro model for the study of basic developmental processes implies large-scale in vitro culture. Here, after a brief description of the main techniques used for karyotype analysis, we will give a detailed overview of the chromosome abnormalities described in pluripotent (embryonic and induced pluripotent SCs) and somatic SCs, and the possible causes of their origin during culture.

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“…Mice parthenogenetic embryonic stem cell lines have been well established (Lee et al, 2008;Ju et al, 2008); however, the potential development of parthenogenetic embryos and the pESC derivation efficiency is still very low (Liu et al, 1998;Cibelli et al, 2002). Therefore, the present study, by the optimization of parthenogenetic embryo production and the aggregation of the parthenogenetic diploid embryo (produced by the optimized method) and the identified male embryo, aimed to investigate a method to improve the development of parthenogenetic embryos and pESC derivation in mice.…”

Section: Discussionmentioning

confidence: 99%

Aggregation of a parthenogenetic diploid embryo and a male embryo improves the blastocyst development and parthenogenetic embryonic stem cell derivation

Qiu¹,

Nan²,

Xiao³

et al. 2017

Turk J Biol

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Parthenogenetically derived mammalian embryos, with no paternal genome, are not viable and die, largely from defective placental growth attributed to a lack of paternal effect, resulting in the low blastulation rate and low derivation efficiency of parthenogenetic embryonic stem cells (pESCs). Therefore, the present study, by the optimization of parthenogenetic embryo production and the aggregation of the parthenogenetic diploid embryo and the identified male embryo, aims to investigate a method to improve the development of parthenogenetic embryo and pESC derivation in mice. Using different chemical combinations for the optimization, we found that the heterozygous diploid type had a significantly higher blastulation rate than the haploid type (P < 0.05). The treatment of strontium chloride (SrCl 2 ) combined with cytochalasin B for 4 h produced the highest heterozygous diploid rate and blastulation rate. Our self-made concave hole system was used for the aggregation of the parthenogenetic heterozygous diploid embryo with the male embryo identified by the duplex PCR method, and we found that the chimeric embryo had an improved rate of blastulation and pESC isolation.

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Establishment of stem cell lines from nuclear transferred and parthenogenetically activated mouse oocytes for therapeutic cloning

Cited by 17 publications

References 29 publications

Effects of chemical combinations on the parthenogenetic activation of mouse oocytes

Effects of chemical combinations on the parthenogenetic activation of mouse oocytes

Chromosomal Abnormalities in Embryonic and Somatic Stem Cells

Aggregation of a parthenogenetic diploid embryo and a male embryo improves the blastocyst development and parthenogenetic embryonic stem cell derivation

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