Establishment of the diploid gynogenetic hybrid clonal line of red crucian carp × common carp

Liu, Shaojun; Duan, Wei; Tao, Min; Zhang, Chun; Sun, Yuena; Shen, Jinhui; Wang, Jing; Luo, Kun; Liu, Yun

doi:10.1007/s11427-007-0032-2

Cited by 39 publications

(32 citation statements)

References 12 publications

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“…Our group has developed gynogenetic strains from natural origin red crucian carp, Carassius auratus cuvieri, goldfish, common carp, orange ornamental carp (Cyprinus carpio), grass carp, Erythroculter ilishaeformis, Xenocypris davidi Bleeker, and artificially cultivated strains including tetraploid hybrids of common carp with red crucian carp and tetraploid hybrids of red crucian carp with blunt snout bream [103][104][105][106][107]. We have developed gynogenetic strains of red crucian carp, Japanese crucian carp, and goldfish following activation using heterogenous sperms (M. amblycephala).…”

Section: Artificial Gynogenesis and Androgenesismentioning

confidence: 99%

“…The survived fish that underwent the adversity were easy to obtain traits that resist harsh conditions. Liu et al [106] have obtained diploid gynogenetic offspring by using the diploid eggs from allotetraploid hybrids by gynogenesis without chromosome-doubling treatment. Through long-term research, we established a strain of diploid gynogenetic hybrids (G 1 -G 8 ) that were able to produce fertile diploid eggs.…”

Section: Artificial Gynogenesis and Androgenesismentioning

confidence: 99%

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Development and application of biological technologies in fish genetic breeding

Duan

Xiao

et al. 2015

Sci. China Life Sci.

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Fish genetic breeding is a process that remolds heritable traits to obtain neotype and improved varieties. For the purpose of genetic improvement, researchers can select for desirable genetic traits, integrate a suite of traits from different donors, or alter the innate genetic traits of a species. These improved varieties have, in many cases, facilitated the development of the aquaculture industry by lowering costs and increasing both quality and yield. In this review, we present the pertinent literatures and summarize the biological bases and application of selection breeding technologies (containing traditional selective breeding, molecular marker-assisted breeding, genome-wide selective breeding and breeding by controlling single-sex groups), integration breeding technologies (containing cross breeding, nuclear transplantation, germline stem cells and germ cells transplantation, artificial gynogenesis, artificial androgenesis and polyploid breeding) and modification breeding technologies (represented by transgenic breeding) in fish genetic breeding. Additionally, we discuss the progress our laboratory has made in the field of chromosomal ploidy breeding of fish, including distant hybridization, gynogenesis, and androgenesis. Finally, we systematically summarize the research status and known problems associated with each technology. fish genetic breeding, genetic improvement, biological method, traits, new variety Citation:

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Section: Artificial Gynogenesis and Androgenesismentioning

confidence: 99%

Section: Artificial Gynogenesis and Androgenesismentioning

confidence: 99%

Development and application of biological technologies in fish genetic breeding

Duan

Xiao

et al. 2015

Sci. China Life Sci.

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“…However, few fish can produce gynogenetic progenies, genetically identical or similar to the somatic cells of the mother, without any treatment for the doubling of chromosomes. This may be related to apomixis, premeiotic endoreduplication, or premeiotic endomitosis [Liu et al, 2007[Liu et al, , 2014Morishima et al, 2012;Arai and Fujimoto, 2013].…”

Section: Production Of Tetraploid Gynogenetic Loachmentioning

confidence: 99%

Production of Tetraploid Gynogenetic Loach Using Diploid Eggs of Natural Tetraploid Loach, Misgurnus anguillicaudatus, Fertilized with UV-Irradiated Sperm of Megalobrama amblycephala without Treatments for Chromosome Doubling

Huang

Cao

Tian

et al. 2015

Cytogenet Genome Res

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The gynogenesis phenomenon in nature mainly appears in the reproduction of fish and invertebrates. So far, gynogenesis has been successfully induced in many fish species with the aid of some physical or chemical methods for chromosome doubling. However, few fish can produce gynogenetic progenies, genetically identical or similar to the somatic cells of the mothers, without a treatment for the doubling of chromosomes, which may be related to apomixis, premeiotic endoreduplication, or premeiotic endomitosis. At present, no studies are available about fish with normal ovarian structures producing gynogenetic progenies that could spontaneously double their chromosomes. According to the analyses of flow cytometry, chromosome number, and microsatellites, we found that, with the use of UV-irradiated sperm of blunt snout bream Megalobrama amblycephala, tetraploid loach Misgurnus anguillicaudatus produced tetraploid gynogenetic progenies without any treatments for the doubling of chromosomes. To determine the genetic relationships of gynogenetic progenies and their maternal parent, microsatellite genotyping was conducted. The results indicated that the reason for spontaneous chromosome duplication in gynogenetic progenies may be cytokinesis or inhibition of the extrusion of the second polar body. This is the first report on fish with normal ovarian structures that can produce gynogenetic progenies which spontaneously double their chromosomes and which are genetically identical or similar to the somatic cells of the mothers.

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“…Interestingly, G 1 could also produce many diploid eggs, which could develop into the second gynogenetic diploid hybrid fish (G 2 ) without the treatment for doubling the chromosomes, then G 2 developed into G 3 in the same way. Based on the formation of diploid G 1 , G 2 and G 3 , a new diploid hybrid clonal line had been established [3] . In this study, we obtained tetraploids (A 1 -4n) and triploids (A 1 -3n) by mating males and females of A 0 , suggesting that A 0 could produce diploid gametes.…”

Section: The Advantages Of Androgenesis With Diploid Sperm and The Fomentioning

confidence: 99%

“…Then G 2 developed into G 3 in the same way. The formation of diploid G 1 , G 2 and G 3 indicated that a new diploid hybrid gynogenetic clonal line was established [3] . On the other hand, the diploid sperm produced by allotetraploid hybrids developed into diploid hybrid androgenetic fish (A 0 ) after the fertilization of the diploid sperm with the UV-treated eggs of goldfish, without the treatment for doubling the chromosomes.…”

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The formation of improved tetraploid population of red crucian carp × common carp hybrids by androgenesis

et al. 2007

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Bisexual fertile diploid androgenetic individuals (A(0)) (2n=100) were formed by androgenesis. In this way, the diploid spermatozoa from male allotetraploid hybrids (AT) (4n=200) of red crucian carp (Carassius auratus red var.) (female) x common carp (Cyprinus carpio L.) (male) were used to fertilize the UV-treated haploid eggs of goldfish (Carassius auratus), and living androgenetic diploid fish were developed. The A(0) became sexually mature at the age of 2 years, and they fertilized with each other to form their offspring (A(1)). In this study, we observed the chromosomal number, gonadal structure and appearance of A(1) fish. The results are as follows: (1) In A(1), there were 85% tetraploids (A(1)-4n), 10% triploids (A(1)-3n) and 5% diploids (A(1)-2n), suggesting that diploid A(0) could produce diploid gametes. It was concluded that the formation of diploid gametes generated from diploid A(0) was probably related to the mechanism of pre-meiotic endoreduplication. (2) Among A(1), only A(1)-4n possessed normal ovaries and testes. The mature males of A1-4n produced white semen. Under the electron microscope, the head of diploid sperm generated by A(1)-4n was bigger than that of haploid sperm generated by red crucian carp. In the testes of the A(1)-4n, there were many mature normal spermatozoa with a head bearing plasma membrane and a tail having the typical structure of "9+2" microtubules. Between the head and the tail, there were some mitochondria. The ovaries of A(1)-4n developed well and mainly contained II, III and IV-stage oocytes. The IV-stage oocytes were surrounded by inner and outer follicular cells. The micropyle was observed on the oolemma of follicular cells. There were abundant yolks and plenty of endoplasmic reticulum in the cytoplasm of IV-stage oocytes. Because A(1)-2n and A(1)-3n were distant crossing diploid hybrids and triploid hybrids respectively, they possessed abnormal gonads, and no mature semen and eggs were observed. (3) Compared with allotetraploids, the A(1)-4n fish not only had advantages such as fast growth rate and strong resistibility but also showed some new good performances such as high ratio of body width to body length, smaller heads and shorter tails. These results indicated that androgenesis could produce bisexual fertile tetraploids and improve the shape of allotetraploid hybrids as well, which will be of great significance in both the cell genetics research and fish breeding.

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Establishment of the diploid gynogenetic hybrid clonal line of red crucian carp × common carp

Cited by 39 publications

References 12 publications

Development and application of biological technologies in fish genetic breeding

Development and application of biological technologies in fish genetic breeding

Production of Tetraploid Gynogenetic Loach Using Diploid Eggs of Natural Tetraploid Loach, <b><i>Misgurnus anguillicaudatus,</i></b> Fertilized with UV-Irradiated Sperm of <b><i>Megalobrama amblycephala</i></b> without Treatments for Chromosome Doubling

The formation of improved tetraploid population of red crucian carp × common carp hybrids by androgenesis

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