Establishment of variations in mycorrhizal colonization produced by overlapping a treatment with zinc sulfate over differentiated fertilization

Abstract

More accentuated intensi ication of management from areas with high biodiversity may act to reduce the level of mycorrhizal colonization, while producing a reduction in plant resistance to stress factors. In the context of current climate drought is an common phenomenon, and depreciation of hyphal networks produced by mycorrhizae in root systems of plants increases their susceptibility to drought. In these circumstances, application of zinc over fertilization can increase the mycorrhizal colonization with increasing plant resistance to drought. The main objective of this paper was to analyze the potential for stabilization of colonization based on zinc application as a base for grassland ecosystems balance preservation under changes in the way of fertilization applied to these surfaces. The level of colonization was analysed in roots of Festuca rubra, during 2010-2011, samples being collected from an experiment with 6 graduations of mineral and organic fertilization located in Garda de Sus village, on each variant being additionally applied a zinc sulphate treatment. Application of zinc sulphate over fertilization with manure acts to reduce the level of frequency of colonization in the root system of plants of Festuca rubra, at the end of the growing season. At the same sampling period, a similar phenomenon is recorded under conditions of Eurofertil mezocalc fertilization. The intensity of the colonization varies greatly under the experimental conditions, higher values were observed at the end of the growing season for fertilization based on chemical nitrogen. During the period of plant maximum activity successful fertilizer recipes were without chemical nitrogen supplements in 2010, and NPK fertilization in 2011, these options ensuring the highest levels of colonization intensity.

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“…After this period, the differences of heights between variants were no longer significant. Mycorrhizal colonisation and the extent of hyphae in roots were assessed according the formulas proposed by Trouvelot (http://www2.dijon.inra.fr/mychintec/Protocole/Workshop_Proced ures.html) and completed with colonisation degree (Cdeg %), described by Stoian et al (2014) as the product between frequency and intensity of colonisation. It have been calculated the arbuscular percentages in mycorrhizal fragments (a %) and in radicular system (A %) and the degree of colonisation (Cdeg %) as factors of mycorrhization.…”