ESTIMASI HERITABILITAS DAN RESPONS SELEKSI PERSILANGAN IKAN GURAMI (Osphronemus goramy Lac.)

Sularto, Sularto; Febrianti, Rita; Suharyanto, Suharyanto

doi:10.15578/jra.11.1.2016.23-28

Cited by 3 publications

(3 citation statements)

References 2 publications

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“…The Jambi (GJ) and Kalimantan (GK) gourami used in this study were collected from areas with a significant geographic distance, namely Sumatra Island and Kalimantan Island. In previous research reported by Sularto et al (2016), it was stated that gourami from Kalimantan showed faster growth performance compared to those from Jambi. This is suspected to be due to the fact that gourami from Kalimantan has undergone a relatively strict selection process related to growth characteristics, which may potentially result in the reduction of genes associated with resistance in that population.…”

Section: Discussionmentioning

confidence: 92%

Identification of potential molecular markers for disease resistance in giant gourami through major histocompatibility complex (MHC) II gene analysis

Khasani,

Febrianti,

Sularto

et al. 2024

Fish Aquat Sci

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Research to obtain molecular markers related to the major histocompatibility complex (MHC) gene in both strains of gourami is essential to increase the success of the selection program of disease resistance traits. Using a completely randomized design (CRD), the challenge test consists of four treatments and seven replications. The treatment was Jambi gourami injected with PBS (KJ), Kalimantan gourami injected with PBS (KK), Jambi strain injected with Aeromonas hydrophila (GJ), and Kalimantan strain injected with A. hydrophila (GK). The GJ population was more resistant to A. hydrophila than the GK population. The MHC II gene was detected in both test strains (GJ and GK), both resistant and susceptible fish. However, there were differences in the results of amplifying the MHC II gene in susceptible and resistant fish. Two DNA fragments approximately 400 and 585 bp were detected in the genome of susceptible fish, while in the genome of susceptible fish, only one DNA fragment was detected (400 bp). Therefore, the MHC II gene fragment with a size of about 585 bp can be used as a potential candidate for specific molecular markers to obtain resistance to A. hydrophila bacteria in the giant gourami.

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Section: Discussionmentioning

confidence: 92%

Identification of potential molecular markers for disease resistance in giant gourami through major histocompatibility complex (MHC) II gene analysis

Khasani,

Febrianti,

Sularto

et al. 2024

Fish Aquat Sci

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“…Akibatnya kualitas genetik dari kedua jenis gurami tersebut diduga telah mengalami penurunan sehingga berdampak negatif terhadap performa produksi. Salah satu alternatif untuk mengatasi masalah pertumbuhan yang lambat adalah dengan memperbaiki mutu genetik ikan gurami, perbaikan mutu genetik dapat dilakukan untuk mengatasi kendala kualitas genetik dan pertumbuhan pada ikan gurami (Sularto et al, 2016). Nugroho et al (2012) mengemukakan bahwa usaha perbaikan genetik untuk mempercepat laju pertumbuhan dan aplikasi teknologi yang tepat guna dalam meningkatkan produksi sangat diperlukan, salah satu cara untuk memperbaiki genetis ikan gurame dengan penerapan persilangan atau hibridisasi.…”

Section: Pendahuluanunclassified

KARAKTERISTIK FENOTIPE DAN GENOTIPE IKAN GURAMI, Osphronemus goramy, STRAIN GALUNGGUNG HITAM, GALUNGGUNG PUTIH, DAN HIBRIDANYA

Arifin

Imron

Muslim³

et al. 2017

Jurnal Riset Akuakultur

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ABSTRAKPersilangan antara dua populasi yang berbeda secara genetik lazimnya menghasilkan kombinasi genetik baru. Penelitian ini dilakukan untuk mengevaluasi karakteristik fenotipe dan genotipe ikan gurami strain Galunggung Hitam, Galunggung Putih, dan Hibridanya. Perkawinan dalam galur (GP X GP dan GH X GH) dan persilangan dua arah antara betina GH dan jantan GP (GH X GP), dan resiprokalnya (GP X GH) telah dilakukan di BPBIGN Singaparna. Fenotipe dianalisis berdasarkan metode truss morfometrik dan meristik, sedangkan karakteristik genotipe diamati menggunakan metode PCR-RAPD. Hasil analisis menunjukkan berdasarkan 21 karakter truss morfometrik, centroid hibrida GH X GP lebih tinggi dari centroid ketiga populasi lainnya pada PC-1 yang dicirikan oleh karakter B3, B4, B5, A3, A4, dan A6, dan bersifat intermediate pada PC-2, PC-3, dan PC-4. Berdasarkan delapan karakter meristik, centroid hibrida GH X GP lebih tinggi dari kedua tetua dan hibrida GP X GH pada PC-1 yang dicirikan oleh karakter SPR dan SDA, lebih rendah dari kedua tetua, tetapi lebih tinggi dari populasi GP X GH pada PC-2, dan lebih rendah dari ketiga populasi lainnya pada PC-3 dan PC-4. Polimorfisme dan heterozigositas pada populasi hibrida GH X GP dan GP X GH lebih tinggi daripada kedua tetuanya GH X GH dan GP X GP. Hibridisasi yang dilakukan meningkatkan variasi genetik yang dapat berguna dalam peningkatan produktivitas budidaya. B3, B4, B5,A3, A4, and A6,. Based on the eight meristic characters, the centroid of GH X GP was also higher than those of their parents and hybrids GP X GH at PC-1, which was marked by the characters SPR and SDA, lower than those of their parents but higher than that of hybrid GP X GH at PC-2, and lower than those of the other three populations at PC-3 and PC-4. The genetic diversities in terms of polymorphism and heterozygosity levels in hybrids GH X GP and GP X GH were higher than those found in the pure-line (GH X GH and GP X GP). Hybridization conducted in this study had resulted in the enhancement of genetic variations which could be useful in increasing aquaculture productivity. Hybridization between two genetically different populations is expected to generate a population carrying new genetic combinations which may be expressed in both phenotypes and genotypes. This study was carried out to explore the phenotype and genotype characteristics of reciprocal hybrids of goramy of Galunggung black (GH) and Galunggung white (GP) with respect to their parental lines. Matings within the same line producing GP X GP and GH X GH, and reciprocal mating producing hybrids GH X GP and GP X GH, were conducted at BPBIGN Singaparna. Samples representing the four populations were analyzed for their morphology using truss morphometric and meristic methods, while genotypes were analyzed using PCR-RAPD method. The results showed that based on the 21 morphometric characters, the centroid of hybrid GH X GP was higher than those of the other three populations at PC-1, which was marked by the characters

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“…Giant gourami fish (Osphronemus gourami) has a growth pattern where males grow faster than females [2]. It takes a between family to process the selection.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Grading on Population Growth Performance for Giant Gourami (Osphronemus gourami) in Selection Program

Sularto¹,

Hadie

Febrianti³

et al. 2021

E3S Web Conf.

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Selection programs applied to fish commodities can be done through some structured stages. The grading process is one of the critical stages of the selection program. Grading can be done by selecting a group of fish in a population that has the best growth performance. Fish groups that selection criteria for the next are maintained populations. The purpose of the study was to determine the effectiveness of grading on the growth of the gourami. This research conducted strains of giant 1gourami Jambi, Kalimantan, Majalengka, and Tasikmalaya strains.The first grading with fish body weight criteria was carried out by the time the gourami population was 11 months old, so it was obtained sub population in three sizes. Each strain is then nurtured separately. After each population months-old gourami, each individual was tagged, then maintained communally until 25 months old. Results of the experiment suggest that grading is apparently effective in affecting giant gourami growth significantly differences (P< 0.05). The implications of this research can be applied in the huge gourami selection program to produce superior strain.

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ESTIMASI HERITABILITAS DAN RESPONS SELEKSI PERSILANGAN IKAN GURAMI (Osphronemus goramy Lac.)

Cited by 3 publications

References 2 publications

Identification of potential molecular markers for disease resistance in giant gourami through major histocompatibility complex (MHC) II gene analysis

Identification of potential molecular markers for disease resistance in giant gourami through major histocompatibility complex (MHC) II gene analysis

KARAKTERISTIK FENOTIPE DAN GENOTIPE IKAN GURAMI, Osphronemus goramy, STRAIN GALUNGGUNG HITAM, GALUNGGUNG PUTIH, DAN HIBRIDANYA

Effectiveness of Grading on Population Growth Performance for Giant Gourami (Osphronemus gourami) in Selection Program

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