Estimates of outcrossing rates in Moringa oleifera using Amplified fragment length polymorphism (AFLP)

Muluvi, Geoffrey M.; Sprent, Janet I.; Odee, David W.; Powell, W.

doi:10.5897/ajb2004.000-2026

Cited by 28 publications

(25 citation statements)

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“…An assumption of intermediate allele frequencies is also expected (Gaiotto et al 1997). Departures from these assumptions, mainly in pollen pool homogeneity, have been reported by Gaiotto et al (1997), Sebbenn et al (1998), Muluvi et al (2004, Gusson et al (2006) and Muchugi et al (2008).The multilocus estimation of 0.804 is close to the 0.74 value reported by Souza (2000) using three isozyme systems. in the Stacy et al (1996) used five isozyme systems to determine that S. mombin was a completely allogamous species with 100% outcrossing.…”

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confidence: 68%

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An AFLP estimation of the outcrossing rate of <i>Spondias tuberosa</i> (Anacardiaceae), an endemic species to the Brazilian semiarid region

Santos¹,

Gama²

2013

RBT

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Una estimación AFLP de la tasa de fecundación cruzada de Spondias tuberosa (Anacardiaceae), una especie endémica de la región semiárida de Brasil. The umbu tree (Spondias tuberosa) is one of the most important endemic species to the Brazilian tropical semiarid region. The umbu tree has edible fruits with a peculiar flavor that are consumed in natura or in a semi-industrialized form, such as jams, candies and juices. The majority of endemic species to Brazilian semiarid region have not been studied or sampled to form germplasm collections, which increases the risk of losing genetic variability of the adapted species to xerophytic conditions. The aim of this study was to estimate outcrossing rates in S. tuberosa using a multilocus mixed model in order to guide genetic resources and breeding programs of this species. DNA samples were extracted from 92 progenies of umbu trees, which were distributed among 12 families. These trees were planted by seed in 1991 in Petrolina, PE, Brazil. The experimental design was a randomized block, with a total of 42 progenies sampled in three regions. The experimental units were composed by five plants and five replications. The outcrossing rate was estimated by the multilocus model, which is available in the MLTR software, and was based on 17 polymorphic AFLP bands obtained from AAA_CTG and AAA_CTC primer combinations. The observed heterozygotes ranged from 0.147 to 0.499, with a maximum frequency estimated for the AAA_CTC_10 amplicon. The multilocus outcrossing estimation ( ) was 0.804±0.072, while the single-locus ( ) was 0.841±0.079, which suggests that S. tuberosa is predominantly an outcrossing species. The difference between and was -0.037±0.029, which indicates that biparental inbreeding was nearly absent. The mean inbreeding coefficient or fixation index ( ) among maternal plants was -0.103±0.045, and the expected was 0.108, which indicates that there was no excess of heterozygotes in the maternal population. The outcrossing estimates obtained in the present study indicate that S. tuberosa is an open-pollinated species. Biometrical models applied to this species should therefore take into account the deviation from random outcrossing to estimate genetic parameters and the constitution of broad germplasm samples to preserve the genetic variability of the species. Outcrossing rates based on AFLP and the mixed-mating model should be applied to other studies of plant species in the Brazilian semiarid region. Rev. Biol. Trop. 61 (2): 577-582. Epub 2013 June 01.Key words: outcrossing rate, MLTR, heterozygosis.The umbu tree (Spondias tuberosa Arruda -Anacardiaceae) is a xerophytic tree species that is endemic to the tropical Brazilian semiarid region (Prado & Gibbs 1993). The high drought tolerance of this species is due to its specialized root structure (xylopodium), which plays a key role in long-term water storage. The tree has edible fruits with a peculiar flavor that are consumed in natura or semi-industrialized in different forms, such as jams, candies and juices.T...

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confidence: 68%

“…Subsequently, DNA molecular markers, such as RAPD and AFLP, have been used to estimate the outcrossing rates of many species in order to increase the number of polymorphic markers in simple reactions (Gaiotto et al 1997, Muluvi et al 2004. Because of their dominance, RAPD and AFLP markers have limitations.…”

mentioning

confidence: 99%

An AFLP estimation of the outcrossing rate of <i>Spondias tuberosa</i> (Anacardiaceae), an endemic species to the Brazilian semiarid region

Santos¹,

Gama²

2013

RBT

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“…This model has been successfully applied by different authors (Gaiotto et al, 1997;Muluvi et al, 2004;Muchugi et al, 2008) to species without available co-dominant markers.…”

Section: Resultsmentioning

confidence: 99%

“…Dominant DNA markers, such as RAPD and AFLP, have been used to estimate outcrossing rates in Moringa oleifera (Muluvi et al, 2004) and Eucalyptus urophylla (Gaiotto et al, 1997), despite limitations on the correct genotypic identification of individuals. To overcome this limitation, Ritland (2002) developed the multilocus model to estimate outcrossing rates in plant species, so that dominant markers could be applied in mating system studies.…”

Section: Introductionmentioning

confidence: 99%

Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers

Santos

Neto

2011

Pesq. agropec. bras.

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− The objective of this work was to estimate outcrossing rates between Haden and Tommy Atkins mango cultivars, using AFLP and microsatellite markers. Progenies of an isolated 'Haden' plant, identified in a 'Tommy Atkins' commercial orchard, in Petrolina, PE, Brazil, were analyzed. Total DNA was isolated from the progeny leaves and used for AFLP and microsatellite reactions. Multilocus outcrossing rates (t m ) were estimated by direct count of AFLP or microsatellite markers and by the mLTR software. Outcrossing rates ranged from 0.85 to 0.87 with the analysis based on seven AFLP markers, and from 0.83 to 0.91 based on three microsatellite primers. No unexpected band patterns were observed for 'Haden' and 'Tommy Atkins'. The estimates obtained with the mLTR software were close to those obtained by direct AFLP and microsatellite allele counting, which indicates that the multilocus model was appropriate for this kind of study. The microsatellites mMiCIR005, mMiCIR030, and mMiCIR036 can be used to elucidate the origin of 'Haden' and 'Tommy Atkins' seedlings.

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“…Microsatellite markers have been used successfully for paternity testing in animals and plants (Riday and Krohn, 2010;Moroni et al, 2011;Sahli and Conner, 2011) and to estimate the outcrossing rate (Kittelson and Maron, 2000;Muluvi et al, 2004;Soengas et al, 2011). The outcrossing and/or selfing rate estimated by molecular markers can be used to verify the effective participation of all parents as pollen donors (male parents) in a polycross.…”

Section: Introductionmentioning

confidence: 99%

Selfing rate estimation in sugarcane under unfavorable natural conditions of crossing by using microsatellite markers

Melloni¹,

Scarpari²,

Pinto³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The self-fertilization or selfing rate estimation using microsatellite markers and its impact on survival and selection rate were evaluated in families derived from polycrosses that involved parents that were widely used in sugarcane breeding in Brazil. These factors were evaluated under unfavorable natural conditions of flowering and crossing. After the germination test, the viable progeny were taken to the field for survival rate evaluation (4, 6, and 10 months) and phenotypic selection at plant cane. The selfing rate estimate based on microsatellite markers present in the progeny and absent in their female parent was 98.5 and 0% for the polycross families derived from IACSP95-5000 and SP89-1115, respectively. The survival and selection rates in the last 2 evaluations were higher for the SP89-1115 outcrossed family than the IACSP95-5000 selfed family. The IACSP95-5000 cultivar excelled either as pollen donor with fertilization capability or viable seed production even under unfavorable natural conditions of crossing. The Selfing rate in sugarcane environment influence (temperature and humidity) had an important role during the polycross.

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Estimates of outcrossing rates in Moringa oleifera using Amplified fragment length polymorphism (AFLP)

Cited by 28 publications

References 44 publications

An AFLP estimation of the outcrossing rate of <i>Spondias tuberosa</i> (Anacardiaceae), an endemic species to the Brazilian semiarid region

An AFLP estimation of the outcrossing rate of <i>Spondias tuberosa</i> (Anacardiaceae), an endemic species to the Brazilian semiarid region

Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers

Selfing rate estimation in sugarcane under unfavorable natural conditions of crossing by using microsatellite markers

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