Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers

Abstract

− The objective of this work was to estimate outcrossing rates between Haden and Tommy Atkins mango cultivars, using AFLP and microsatellite markers. Progenies of an isolated 'Haden' plant, identified in a 'Tommy Atkins' commercial orchard, in Petrolina, PE, Brazil, were analyzed. Total DNA was isolated from the progeny leaves and used for AFLP and microsatellite reactions. Multilocus outcrossing rates (t m ) were estimated by direct count of AFLP or microsatellite markers and by the mLTR software. Outcrossing rates ranged from 0.85 to 0.87 with the analysis based on seven AFLP markers, and from 0.83 to 0.91 based on three microsatellite primers. No unexpected band patterns were observed for 'Haden' and 'Tommy Atkins'. The estimates obtained with the mLTR software were close to those obtained by direct AFLP and microsatellite allele counting, which indicates that the multilocus model was appropriate for this kind of study. The microsatellites mMiCIR005, mMiCIR030, and mMiCIR036 can be used to elucidate the origin of 'Haden' and 'Tommy Atkins' seedlings.

Self-fertility and preferential cross-fertilization in mango ( Mangifera indica )

“…The results show a high outcrossing rate in mango in the line of previous paternity studies using morphological (Singh et al, 1962;Mukherjee et al, 1968;Sharma and Singh, 1970) and molecular traits (Schnell et al, 1994;Dag et al, 1997;Degani et al, 1997b;Dag et al, 1998;Dag et al, 2001;Santos and Neto, 2011). Those results suggest the presence of negative selection after fertilization of fruits resulting from selfing, a similar situation to that also reported in other subtropical fruit crops such as avocado or lychee (Degani et al, 1995;Degani et al, 1997a;Alcaraz and Hormaza, 2011).…”

“…The first studies with molecular markers in mango to evaluate outcrossing rate were performed with isozymes (Dag et al, 1997;Dag et al, 1998;Dag et al, 2009), pointing out a high rate of outcrossing in fruits from plants placed near pollinizer trees. Later, studies continued with microsatellite and AFLP markers showing similar results (Schnell et al, 2006;Santos and Neto, 2011).…”

Inheritance of resistance to dieback disease in mango (Mangifera indica)

“…Mango is a highly heterozygous species, with cross pollination rate of up to 93% (Santos and Lima Neto, 2011), which can be explored for genetic studies. As stated by Banerjee et al (2012) and Parikh et al (2016), F 1 plants resulting from crossings between parents highly heterozygote at many loci is considered as pseudo-F 2 and not as F 1 plants.…”

Molecular genetic diversity and mating system in sacha inchi progenies1

“…Despite the limitations of these markers, due to their dominant nature, the high degree of polymorphism that they usually reveal offsets this limitation in the analysis for determining the characteristics of the reproductive system. Studies such as those by Santos & Lima Neto (2011) and Sharma et al (2017) show how the crossing rates obtained by AFLP markers and SSR markers are very similar in mango varieties (Mangifera indica) and Indian beech (Pongamia pinnata), respectively.…”

“…Amplified Fragment Length Polymorphism (AFLP) markers have been used to estimate crossing rates in open-pollinated progenies of several species (Santos & Lima Neto 2011, Hornemann et al 2012, Santos & Gama 2013, Sharma et al 2017. Despite the limitations of these markers, due to their dominant nature, the high degree of polymorphism that they usually reveal offsets this limitation in the analysis for determining the characteristics of the reproductive system.…”