Estimating diet in individual pumpkinseed sunfish <i>Lepomis gibbosus</i> using stomach contents, stable isotopes and parasites

Locke, Sean A.; Bulté, Grégory; Forbes, Mark R.; Marcogliese, David J.

doi:10.1111/j.1095-8649.2012.03497.x

Cited by 31 publications

(23 citation statements)

References 58 publications

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“…The partial discrepancy in the outputs of the dietary analysis between SIA and GCA is also consistent with the study on the diet of Lepomis gibbosus by Locke et al. (), who found little association in diet composition when compared using three methods, including GCA and SIA. They argued this was due to gut contents providing only a dietary snap‐shot in which slowly digested items (e.g.…”

Section: Discussionsupporting

confidence: 89%

Reproductive ecology and diet of a persistent Ameiurus melas (Rafinesque, 1820) population in the UK

et al. 2014

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Section: Discussionsupporting

confidence: 89%

Reproductive ecology and diet of a persistent Ameiurus melas (Rafinesque, 1820) population in the UK

et al. 2014

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“…This general application of constant discrimination factors across consumers within mixing models is potentially problematic, as studies increasingly suggest they can vary between species, ages, diet compositions, body sizes, sample preparations and tissue types (Locke et al, 2013;Brush et al, 2012;Busst et al, 2015). The commonly cited values of 3.4±0.98‰ for δ 15 N and 0.39±1.3‰ for δ 13 C (DeNiro and Epstein, 1981;Minagawa and Wada, 1984;Post, 2002) could thus be inappropriate for use in many models, resulting in dietary predictions whose variability within and between species could be due more to inappropriate discrimination factors than actual dietary variation (Busst et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

High variability in stable isotope diet-tissue discrimination factors of two omnivorous freshwater fishes in controlled ex situ conditions

Busst

Britton

2016

Journal of Experimental Biology

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Diet-tissue discrimination factors (Δ 13 C and Δ 15 N) are influenced by variables including the tissues being analysed and the taxon of the consumer and its prey. Whilst differences in Δ 13 C and Δ 15N are apparent between herbivorous and piscivorous fishes, there is less known for omnivorous fishes that consume plant and animal material. Here, the omnivorous cyprinid fishes Barbus barbus and Squalius cephalus were held in tank aquaria and exposed to three diets that varied in their constituents ( plant based to fishmeal based) and protein content (13% to 45%). After 100 days and isotopic replacement in fish tissues to 98%, samples of the food items, and dorsal muscle, fin tissue and scales were analysed for δ 13 C and δ 15 N. For both species and all diets, muscle was always enriched in δ 15 N and depleted in δ 13 C compared with fin tissue and scales. Across the different diets, Δ 13 C ranged between 2.0‰ and 5.6‰ and Δ 15N ranged between 2.0‰ and 6.9‰. The diet based on plant material (20% protein) always resulted in the highest discrimination factors for each tissue, whilst the diet based on fishmeal (45% protein) consistently resulted in the lowest. The discrimination factors produced by non-fish diets were comparatively high compared with values in the literature, but were consistent with general patterns for some herbivorous fishes. These outputs suggest that the diet-tissue discrimination factors of omnivorous fishes will vary considerably between animal and plant prey, and these specific differences need consideration in predictions of their diet composition and trophic position.

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“…the isotopic 'discrimination' factor (D; Martínez del Rio & Wolf 2005). The issues that affect discrimination factors within and between consumer species include the age and body size of sampled individuals, their diet composition, the tissues analysed and the sample preparations completed prior to analysis (Brush et al, 2012;Locke et al, 2013;Busst et al, 2015). There can be high uncertainty in the discrimination factors of many species (Moore & Semmens, 2008;Elsdon et al, 2010;Busst & Britton, 2016).…”

Section: Introductionmentioning

confidence: 99%

Stable isotope discrimination factors of omnivorous fishes: influence of tissue type, temperature, diet composition and formulated feeds

Britton

Busst

2017

Hydrobiologia

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Application of stable isotope data to trophic studies requires understanding of factors influencing the isotopic discrimination factor (D) between consumers and their prey resources. This is missing for many omnivorous species, despite their diet and environment potentially impacting D. The effects of temperature, diet (including formulated feeds) and tissue type on D 13 C and D 15 N were thus tested experimentally. A temperature experiment exposed three species to identical diets at 18 and 23°C, whereas a diet experiment exposed one species to four diets at 18°C. At 23°C, C:N ratios, D 13 C and D 15 N were generally elevated versus 18°C. After lipid correction, tissue/species-specific differences at 23°C in D 13C and D 15 N were up to 0.73 and 0.54% higher, respectively. Across the four diets, there were also significant differences in D 13 C and D 15 N between a natural diet and diets based on formulated feeds. D 13 C and D 15 N of muscle were 1.51 to 2.76% and 3.13 to 5.44%, respectively. Highest D for both isotopes was from a formulated feed based on plant material that resulted in lower dietary protein content and quality. Thus, diet and environment fundamentally affected the isotopic discrimination factors and these factors require consideration within trophic studies based on stable isotopes.

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Estimating diet in individual pumpkinseed sunfish Lepomis gibbosus using stomach contents, stable isotopes and parasites

Cited by 31 publications

References 58 publications

Reproductive ecology and diet of a persistent Ameiurus melas (Rafinesque, 1820) population in the UK

Reproductive ecology and diet of a persistent Ameiurus melas (Rafinesque, 1820) population in the UK

High variability in stable isotope diet-tissue discrimination factors of two omnivorous freshwater fishes in controlled ex situ conditions

Stable isotope discrimination factors of omnivorous fishes: influence of tissue type, temperature, diet composition and formulated feeds

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