Estimating intramuscular fat content and fatty acid composition in live and post-mortem samples in pigs

Bosch, L.; Tör, M.; Reixach, Josep Peñuelas i; Estany, J.

doi:10.1016/j.meatsci.2009.02.013

Cited by 35 publications

(29 citation statements)

References 25 publications

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“…Fat content and fatty composition was determined in duplicate by quantitative determination of the individual fatty acids by gas chromatography [45]. Fatty acid methyl esters were directly obtained by transesterification using a solution of 20% boron trifluoride in methanol and then determined by gas chromatography using a capillary column SP2330 (30 m × 0.25 mm, Supelco, Bellefonte, PA).…”

Section: Methodsmentioning

confidence: 99%

A Functional Variant in the Stearoyl-CoA Desaturase Gene Promoter Enhances Fatty Acid Desaturation in Pork

et al. 2014

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There is growing public concern about reducing saturated fat intake. Stearoyl-CoA desaturase (SCD) is the lipogenic enzyme responsible for the biosynthesis of oleic acid (18∶1) by desaturating stearic acid (18∶0). Here we describe a total of 18 mutations in the promoter and 3′ non-coding region of the pig SCD gene and provide evidence that allele T at AY487830:g.2228T>C in the promoter region enhances fat desaturation (the ratio 18∶1/18∶0 in muscle increases from 3.78 to 4.43 in opposite homozygotes) without affecting fat content (18∶0+18∶1, intramuscular fat content, and backfat thickness). No mutations that could affect the functionality of the protein were found in the coding region. First, we proved in a purebred Duroc line that the C-T-A haplotype of the 3 single nucleotide polymorphisms (SNPs) (g.2108C>T; g.2228T>C; g.2281A>G) of the promoter region was additively associated to enhanced 18∶1/18∶0 both in muscle and subcutaneous fat, but not in liver. We show that this association was consistent over a 10-year period of overlapping generations and, in line with these results, that the C-T-A haplotype displayed greater SCD mRNA expression in muscle. The effect of this haplotype was validated both internally, by comparing opposite homozygote siblings, and externally, by using experimental Duroc-based crossbreds. Second, the g.2281A>G and the g.2108C>T SNPs were excluded as causative mutations using new and previously published data, restricting the causality to g.2228T>C SNP, the last source of genetic variation within the haplotype. This mutation is positioned in the core sequence of several putative transcription factor binding sites, so that there are several plausible mechanisms by which allele T enhances 18∶1/18∶0 and, consequently, the proportion of monounsaturated to saturated fat.

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Section: Methodsmentioning

confidence: 99%

A Functional Variant in the Stearoyl-CoA Desaturase Gene Promoter Enhances Fatty Acid Desaturation in Pork

et al. 2014

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“…Methyl esters were determined by gas chromatography using a capillary column SP2330 (30 m×0.25 mm fused silica capillary coated with a 0.20 μm film of poly 80% bicyanopropyl-20% cyanopropylphenyl siloxane, Supelco, Bellefonte, PA) and a flame ionization detector with helium as carrier gas at 1 mL/min. The oven temperature program increased from 150 to 225°C at 7°C per min and injector and detector temperatures were both 250°C, (Bosch, Tor, Reixach, & Estany, 2009). Response factors of methyl esters were calculated under the same chromatographic conditions using a commercially available standard mixture of FAME.…”

Section: Analysis Of Fatty Acid Composition By Gas Chromatographymentioning

confidence: 99%

Hepatic lipogenic enzyme expression in pigs is affected by selection for decreased backfat thickness at constant intramuscular fat content

et al. 2013

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“…Once minced, a small quantity of SB and IMF samples was used to determine dry matter by drying 24 h at 100-102 1C in an air oven, whereas the rest of the sample was freeze-dried. Fatty acid methyl esters were directly obtained by transesterification using a solution of boron trifluoride 20% in methanol (Rule, 1997) and determined by gas chromatography (Bosch et al, 2009). Intramuscular fat content was calculated as the sum of individual FA expressed as triglyceride equivalents on a dry tissue basis.…”

Section: Laboratory Analysesmentioning

confidence: 99%

Relationship between blood lipid indicators and fat content and composition in Duroc pigs

2012

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Estimating intramuscular fat content and fatty acid composition in live and post-mortem samples in pigs

Cited by 35 publications

References 25 publications

A Functional Variant in the Stearoyl-CoA Desaturase Gene Promoter Enhances Fatty Acid Desaturation in Pork

A Functional Variant in the Stearoyl-CoA Desaturase Gene Promoter Enhances Fatty Acid Desaturation in Pork

Hepatic lipogenic enzyme expression in pigs is affected by selection for decreased backfat thickness at constant intramuscular fat content

Relationship between blood lipid indicators and fat content and composition in Duroc pigs

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