Estimating the state of population gene pools of the specially protected Helicopsis striata (Mollusca, Gastropoda, Pulmonata) species based on DNA markers

Snegin, Eduard A.

doi:10.1134/s2079059717020113

Cited by 7 publications

(7 citation statements)

References 16 publications

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“…The level of genetic differentiation of Roman snail populations was considerably lower than that of the populations of the relict mollusk Helicopsis striata Mull. (Ost = 0.404, Nm = 0.360, Var/Vwp = 40/60%), also found in the study area [38].…”

Section: Resultssupporting

confidence: 76%

Population genetic structure of Helix pomatia L. (Mollusca, Pulmonata) from the southeastern and eastern parts of the range

Snegin

Artemchuk

2017

Russ J Genet

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On the basis of the analysis of genetic variation detectable by ISSR-PCR, the state of the gene pools of 14 populations of Roman snail Helix pomatia L. in the conditions of urbanized landscapes of the southeastern and eastern parts of the current range was examined. According to the data obtained, the major ity of the studied populations of this mollusk are in satisfactory condition. This is evidenced by the increased level of genetic diversity (He = 0.199 ± 0.025, / sh = 0.306 ± 0.035) and the high values of effective popula tion size, calculated, on the basis of the straight-line regression equation, between the pairwise genetic and geographic distances (Ne = 2.0-4.9) that are comparable with indigenous common species of terrestrial mollusks. Despite the high level of differentiation (Gst = 0.255, Фй = 0.233, Nm = 0.822), the population distri bution was not random (Rm =-0.591, p = 0.0004) and corresponded to the model of isolation by distance. It is hypothesized that, in the adventitious colonies of this mollusk, effective formation of a balanced genetic structure takes place that, in the context of biological and ecological features, facilitates its adaptation to the conditions of an urban environment and the population of the new territories of Eastern Europe.

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Section: Resultssupporting

confidence: 76%

Population genetic structure of Helix pomatia L. (Mollusca, Pulmonata) from the southeastern and eastern parts of the range

Snegin

Artemchuk

2017

Russ J Genet

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“…Studies of the genetic structure of the adventitious populations of H. pomatia in Eastern Europe using the ISSR m ethod based on capillary electrophoresis make it possible to m ore accurately estimate the level of genetic diversity and the subdivision of groups of snails. The values of the effective num ber of Rom an snail populations determ ined using the Slatkin model significantly exceed the similar indices we obtained earlier in populations of mollusc species aboriginal for the area under study [5,16,17], which indicates their high viability. The latter is likely to contribute to suc cessful colonization by Rom an snails of the areas out side their native range.…”

Section: Co N Clu Sio N Ssupporting

confidence: 51%

Estimation of the Genetic Structure of Helix pomatia L. Populations (Mollusca, Pulmonata) by Capillary Electrophoresis of ISSR DNA Fragments

2018

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Using the capillary electrophoresis of ISSR DNA fragments, the genetic structure of the adventi tious populations of Helix pomatia L. (Mollusca, Pulmonata) inhabiting the territory of Eastern Europe was studied. A significant decrease in genetic heterogeneity (He = 0.095 ± 0.002) and population differentiation level (Fst = 0.074) was revealed in comparison with similar data obtained using the ISSR method with elec trophoretic detection in agarose gel and allozymes in polyacrylamide gel. Similar to other methods, an effect of spatial isolation (RM =-0.476) was found on the basis of regression analysis between genetic and geo graphical distances. The effective population size, calculated with the help of M. Slatkin's model, turned out to be the largest of the previously calculated values (Ne = 41.8), which indicates the high viability of the stud ied groups of Roman snails in newly developed territories.

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“…As an example, we used data on the frequencies of DNA locus alleles (RAPD and ISSR) from our previous studies of the population structure of two specially protected species: a sedentary terrestrial steppe snail Helicopsis striata (9 populations) [4] and a highly-dapper stag-beetle Lucanus cervus (4 populations), [5,6] inhabiting the south of the Central Russian Upland.…”

Section: Methodsmentioning

confidence: 99%

“…[7] EXPERIMENTAL The distribution pattern of multi-locus genotypes in the populations under study is shown in Figures 1 and 2, and the calculated indicators of potential genetic diversity are presented in the Table 1. According to the obtained data, the 7 th and 8 th groups stand out the most strongly among the populations of H. striata. 8 -Divnogor'e, 9 -Yamskaya steppe [4] These populations inhabit the relict biocenoses. In these groups, the greatest number of multi-locus genotypes was noted, which turned out to be unique for them.…”

Section: Original Articlementioning

confidence: 99%

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Snegin¹

2017

IJGP

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Aim: The work presents the algorithm for calculating the potential number of genetic combinations with an increase in the animal population to infinity. Methods: Calculations were carried out using the methods of bias corrected form for the Chao 1 and the 1 st order jackknife, based on the multi-locus variability of DNA markers (RAPD and ISSR) in two specially protected species of Lucanus cervus (stag-beetle) and Helicopsis striata (steppe snail). Results: According to the obtained data, the groups, inhabiting relict biocenoses, are the most prominent among the populations of two species. There is the largest number of multi-locus genotypes, most of which are unique.The most monomorphic are the populations, inhabiting technogenic territories, because strongly isolated and small groups of animals are formed here very often. Their drift of genes leads to a decrease in allelic diversity. Conclusion: In addition, it has been established, that dapper animals (insects) are better adapted for existence in anthropogenic environment, than sedentary species (snails).

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Estimating the state of population gene pools of the specially protected Helicopsis striata (Mollusca, Gastropoda, Pulmonata) species based on DNA markers

Cited by 7 publications

References 16 publications

Population genetic structure of Helix pomatia L. (Mollusca, Pulmonata) from the southeastern and eastern parts of the range

Population genetic structure of Helix pomatia L. (Mollusca, Pulmonata) from the southeastern and eastern parts of the range

Estimation of the Genetic Structure of Helix pomatia L. Populations (Mollusca, Pulmonata) by Capillary Electrophoresis of ISSR DNA Fragments

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