Infectious bursal disease (IBD) is an acute and highly infectious and contagious disease that affects young chickens. The current study aims to determine the seroprevalence of IBD in healthy, non-vaccinated male chickens raised as backyard chickens and histopathological changes. We collected 210 tissues and blood samples from backyard chickens in Amman, Jordan. Indirect ELISA was applied to test serum samples for IBD virus antibodies. Morbid tissue samples of livers and spleens were processed for histopathological examination. The study revealed 80.95% prevalence rates of infectious bursal disease virus (IBDV) antibodies. Seropositivity to IBDV did not vary significantly between the study sites. It was concluded that IBD is endemic and broadly appeared in the studied areas. Histopathological study revealed that IBD rendered depletion of lymphoid tissue in the spleen. The franked disease did not occur in adult and older chickens, though those were also seropositive for IBD virus antibodies.