2017
DOI: 10.7324/japs.2017.71116
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Estimation of Bosentan Monohydrate in Male Rabbit Plasma by using RP-HPLC Method

Abstract: A new method for the quantitative determination of bosentan monohydrate in male rabbit plasma was developed by Reverse Phase HPLC as per standard regulatory guidelines. Precipitation method was used for extracting the drug from the drug spiked plasma using mobile phase. The analyte extracted was injected in to ODS column C18 (250 x 4.6 mm, 5µm), temperature was maintained at 25 0 C and monitoring of effluent was performed at 273 nm. The mobile phase consist acetonitrile and phosphate buffer (pH 4.6) in 80:20 r… Show more

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“…Although the new HPLC machines have high repeatability injectors, the internal standard technique is utilized to mitigate the error of sample preparation and injection 30 , However, it is crucial to establish a novel approach in order to more accurately detect Bosentan in plasma. Etodolac has been used as an internal standard to measure Bosentan in plasma in previous studies 31 . One of the most important uses of Bosentan is to control pulmonary blood pressure, and most patients use Losartan to control blood pressure, therefore, in this work Losartan was used as an internal standard 15 .…”
Section: Discussionmentioning
confidence: 99%
“…Although the new HPLC machines have high repeatability injectors, the internal standard technique is utilized to mitigate the error of sample preparation and injection 30 , However, it is crucial to establish a novel approach in order to more accurately detect Bosentan in plasma. Etodolac has been used as an internal standard to measure Bosentan in plasma in previous studies 31 . One of the most important uses of Bosentan is to control pulmonary blood pressure, and most patients use Losartan to control blood pressure, therefore, in this work Losartan was used as an internal standard 15 .…”
Section: Discussionmentioning
confidence: 99%