Estimation of chromium-51 ethylene diamine tetra-acetic acid plasma clearance: a comparative assessment of simplified techniques

Abstract

Chromium-51 ethylene diamine tetra-acetic acid (51Cr-EDTA) total plasma clearance was evaluated using a multi-sample method (i.e. 12 blood samples) as the reference compared with several simplified methods which necessitated only one or few blood samples. The following 5 methods were evaluated: terminal slope-intercept method with 3 blood samples, simplified method of Bröchner-Mortensen and 3 single-sample methods (Constable, Christensen and Groth, Tauxe). Linear regression analysis was performed. Standard error of estimate, bias and imprecision of different methods were evaluated. For 51Cr-EDTA total plasma clearance greater than 30 ml.min-1, the results which most approximated the reference source were obtained by the Christensen and Groth method at a sampling time of 300 min (inaccuracy of 4.9%). For clearances between 10 and 30 ml.min-1, single-sample methods failed to give reliable results. Terminal slope-intercept and Bröchner-Mortensen methods were better, with inaccuracies of 17.7% and 16.9%, respectively. Although sampling times at 180, 240 and 300 min are time-consuming for patients, 51Cr-EDTA total plasma clearance can be accurately calculated for values greater than 10 ml.min-1 using the Bröchner-Mortensen method. In patients with clearance greater than 30 ml.min-1, single-sample techniques provide a good alternative to the multi-sample method; the choice of the method to be used depends on the degree of accuracy required.

Do Healthy Potential Kidney Donors in India Have an Average Glomerular Filtration Rate of 81.4 ml/min?

“…Picciotto et al [23] compared simplified one-sample methods with the two-compartment method and concluded that the Christensen and Groth [24] iterative method (using a single 5-hour sample) as the most accurate method. Gamma camera-based imaging methods for determination of GFR have been proposed by Piepsz et al [25] and Gates [26].…”

Hyperfiltration Affects Accuracy of Creatinine eGFR Measurement

“…However, nuclear medicine studies have replaced inulin clearance because of convenience and absence of urine collection. In Europe, 51 Cr-EDTA is the most widely used method for the determination of GFR (3), whereas in North America, the 99 Tc-diethylenetriamine pentaacetic acid renal scan enjoys the most widespread utilization (3)(4)(5). Although less demanding than inulin clearance studies, nuclear GFR studies are still cumbersome, invasive, and involve radiation.…”

Assessment of Glomerular Filtration Rate Measurement with Plasma Sampling: A Technical Review

“…Although ECV is rarely used, it has been shown to be superior to BSA, especially in pediatric patients (32,33) where BSA is in meters squared, height is in centimeters, and weight is in kilograms. For 1-compartment characterization, it is important to perform BSA normalization before applying the quadratic Bröchner-Mortensen correction for the missing earlycompartment correction (10). The Bröchner-Mortensen correction is known to have the effect of overestimating GFR for patients with a GFR of above 80 mL/min/1.73 m 2 , particularly for patients with a BSA below 1 m 2 , such as in children.…”