Estimation of Decellularization and Recellularization Quality of Tissue-Engineered Constructions by the Chemiluminescence Method

Preclinical Development of Bioengineered Allografts Derived from Decellularized Human Diaphragm

“…This protocol allowed viable and functional scaffolds to be obtained, with high biocompatibility, pro-angiogenic, and pro-regenerative potential, which demonstrated to be re-innervated after implantation into a GFP+ Schwann cell mouse model [27][28][29][30]. The same detergentenzymatic treatment proved to be effective on producing acellular rat diaphragm [31], which was also efficiently decellularized by muscle perfusion via vena cava [32,37,38], or immersion [33,36] in solutions of SDS, DNase-I, and ethylenediaminetetraacetic acid (EDTA). Another documented protocol for rat diaphragm decellularization was based on the use of SDS and PBS washes [34], while Sesli and collaborators [35] demonstrated that the use of SDS+Triton X-100 produced cytocompatible acellular scaffolds in comparison with freezing/thawing method plus immersion in sodium chloride (NaCl), which caused severe tissue damage.…”

“…In this work, human diaphragm harvested from cadavers was considered as an ideal, but still little investigated, source for the development of decellularized biological scaffolds for VML treatment. To the best of our knowledge, only 13 works in the literature have reported the decellularization of the diaphragmatic muscle [27][28][29][30][31][32]34,[39][40][41][42][43][44], plus 5 research articles that considered the diaphragm together with other tissues for methodological studies about decellularization procedures or analysis techniques validating acellular ECM quality [33,[35][36][37][38] (Table S1).…”

“…In particular, human diaphragmatic skeletal muscle may represent a favourable tissue source to obtain biological scaffolds with myogenic potential. The decellularization of mouse [27][28][29][30], rat [31][32][33][34][35][36][37][38], bovine [39,40], and pig [41][42][43] diaphragm was previously reported to fabricate biological prosthesis mainly intended for orthotopic implants in the regeneration of diaphragmatic defects [27,28,32], including neonatal congenital hernia [29][30][31]34,43]. Regarding different clinical targets, the development of decellularized diaphragmatic muscle was also conceptualised for chronic wounds treatment [41], abdominal wall repair [39,40], and anterior cruciate ligament reconstruction [42].…”