Estimation of energy of the upper electron-excited states of the bacterial bioluminescent emitter

Kudryasheva, Nadezhda S.; Nemtseva, Elena V.; Sizykh, A. G.; Kratasyuk, Valentina A.; Visser, Antonie J. W. G.

doi:10.1016/s1011-1344(02)00360-3

Cited by 14 publications

(13 citation statements)

References 8 publications

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“…Five mechanisms are discussed: (1) change of electron-excited states' population and energy transfer processes in the presence of exogenous molecular energy acceptors; (2) change of the efficiency of S-T conversion in bioluminescent emitter in the presence of external heavy-halogen-substituted compounds; (3) change of rates of coupled enzymatic redox reactions under exposure to oxidizers; (4) interactions of hydrophobic and/or halogensubstituted compounds with enzymes and variation, by this, the enzymatic activity; and (5) non-specific effects of electron acceptors. The effects of different groups of exogenous compounds-organic dyes (Nemtseva and Kudryasheva 2007;Kudryasheva et al 2004;2003b), oxidizers (Vetrova et al 2009;2007;, halogen-substituted molecules (Kirillova et al 2011;Kirillova and Kudryasheva 2007;Gerasimova and Kudryasheva 2002;Kudryasheva et al 2002b), and salts of stable and radioactive metals Kamnev et al 2013;Tarasova et al 2012;Alexandrova et al 2011;Rozhko et al 2007;Kudryasheva et al 1999 b;1996) are discussed according to the classification suggested.…”

Section: Environmental Toxicity Monitoring and Bacterial Bioluminescementioning

confidence: 99%

Pollutant toxicity and detoxification by humic substances: mechanisms and quantitative assessment via luminescent biomonitoring

Kudryasheva

Tarasova

2014

Environ Sci Pollut Res

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The paper considers mechanisms of detoxification of pollutant solutions by water-soluble humic substances (HSs), natural detoxifying agents. The problems and perspectives of bioassay application for toxicity monitoring of complex solutions are discussed from ecological point of view. Bioluminescence assays based on marine bacteria and their enzymes are of special attention here; they were shown to be convenient tools to study the detoxifying effects on cellular and biochemical levels. The advantages of bioluminescent enzymatic assay for monitoring both integral and oxidative toxicities in complex solutions of model pollutants and HS were demonstrated. The efficiencies of detoxification of the solutions of organic oxidizers and salts of metals (including radioactive ones) by HS were analyzed. The dependencies of detoxification efficiency on time of exposure to HS and HS concentrations were demonstrated. Antioxidant properties of HS were considered in detail. The detoxifying effects of HS were shown to be complex and regarded as 'external' (binding and redox processes in solutions outside the organisms) and/or 'internal' organismal processes. The paper demonstrates that the HS can stimulate a protective response of bacterial cells as a result of (1) changes of rates of biochemical reactions and (2) stabilization of mucous layers outside the cell walls. Acceleration of auto-oxidation of NADH, endogenous reducer, by HS was suggested as a reason for toxicity increase in the presence of HS due to abatement of reduction ability of intracellular media.

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Section: Environmental Toxicity Monitoring and Bacterial Bioluminescementioning

confidence: 99%

Pollutant toxicity and detoxification by humic substances: mechanisms and quantitative assessment via luminescent biomonitoring

Kudryasheva

Tarasova

2014

Environ Sci Pollut Res

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“…53 ± 55 In particular, the energy transfer to exogenous molecules introduced in the bioluminescent reaction has been attempted. 53,54 Fluorescent aromatic molecules with energies of the first singlet excited state in the range of 22 000 ± 32 000 cm 71 (i.e., higher than the analogous energy of the emitter molecule) have been used as the exogenous molecules (potential acceptors of excitation energy). Note that the absorption spectra of the chosen molecules did not overlap with the bioluminescence spectrum.…”

Section: Introductionmentioning

confidence: 99%

“…Since the sensitised luminescence was detected only for compounds with an energy of the fluorescent state of not more than 26 000 cm 71 , this energy was taken as the characteristics of the upper electron-excited state of the bacterial bioluminescence emitter. 54 A study of the fluorescence anisotropy decay of the acceptor molecules in the presence of bacterial luciferase proved the possibility of hydrophobic binding of acceptor molecules to luciferase, and, hence, these molecules can approach the emitter to distances appropriate for energy transfer by the exchange mechanism. 55 The last mechanism of bacterial bioluminescence to be mentioned was proposed by McCapra.…”

Section: Introductionmentioning

confidence: 99%

The mechanism of electronic excitation in the bacterial bioluminescent reaction

Nemtseva¹,

Kudryasheva²

2007

Russ. Chem. Rev.

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“…They concluded that this eliminated any excited carbonyl product playing the role of the HEI. However, these parinaldehydes have their lowest energy absorption band maximum at 320 nm, exceeding the energy limit for sensitization by the effective dyes used by Kudreyasheva and coworkers (189). Fluorescence anisotropy did not indicate specific association of the sensitizing dyes with Lux, so the authors speculated that the excitation mechanism was through a collisional complex (77,190).…”

Section: The High Energy Intermediatementioning

confidence: 91%

The Sensitized Bioluminescence Mechanism of Bacterial Luciferase

Lee

Müller²,

Visser

2018

Photochem & Photobiology

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After more than one‐half century of investigations, the mechanism of bioluminescence from the FMNH2 assisted oxygen oxidation of an aliphatic aldehyde on bacterial luciferase continues to resist elucidation. There are many types of luciferase from species of bioluminescent bacteria originating from both marine and terrestrial habitats. The luciferases all have close sequence homology, and in vitro, a highly efficient light generation is obtained from these natural metabolites as substrates. Sufficient exothermicity equivalent to the energy of a blue photon is available in the chemical oxidation of the aldehyde to the corresponding carboxylic acid, and a luciferase‐bound FMNH‐OOH is a key player. A high energy species, the source of the exothermicity, is unknown except that it is not a luciferin cyclic peroxide, a dioxetanone, as identified in the pathway of the firefly and the marine bioluminescence systems. Besides these natural substrates, variable bioluminescence properties are found using other reactants such as flavin analogs or aldehydes, but results also depend on the luciferase type. Some rationalization of the mechanism has resulted from spatial structure determination, NMR of intermediates and dynamic optical spectroscopy. The overall light path appears to fall into the sensitized class of chemiluminescence mechanism, distinct from the dioxetanone types.

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Estimation of energy of the upper electron-excited states of the bacterial bioluminescent emitter

Cited by 14 publications

References 8 publications

Pollutant toxicity and detoxification by humic substances: mechanisms and quantitative assessment via luminescent biomonitoring

Pollutant toxicity and detoxification by humic substances: mechanisms and quantitative assessment via luminescent biomonitoring

The mechanism of electronic excitation in the bacterial bioluminescent reaction

The Sensitized Bioluminescence Mechanism of Bacterial Luciferase

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