Six Japanese Black (Wagyu) steers (average initial weight 467 Ϯ 45 kg) fitted with a ruminal cannula were used in a split-plot design experiment comprising a 3 ¥ 3 Latin square design (whole-plot) and a randomized block design (subplot). The whole-plot treatments were three different feeding levels of urea-treated potato pulp (PP) silage-based concentrate: 1.00%, 1.75% and 2.50% of body weight (BW) (on a dry matter (DM) basis). The subplot treatments consisted of the concentrate formulated to contain either soybean meal (SBM) as a rapidly rumen-degraded protein source or corn gluten meal (CGM) as a slowly degraded protein source. Dry matter intake tended to be lower (P = 0.071) for CGM (8.9 kg/day) than for SBM (9.4 kg/day). Protein sources had no significant effect on digestibility and in situ degradation. Ruminal ammonia nitrogen (NH3-N) was lower (P = 0.033) for CGM (7.5 mg/dL) than for SBM (9.5 mg/dL). Protein sources did not affect ruminal pH and the total volatile fatty acids (VFA) concentrations. The molar proportions of ruminal acetate and valerate were higher (P = 0.032) for CGM than for SBM. The maximum daily intake of the PP silage-based concentrate expressed as a percentage of BW was approximately 1.4% of BW. Dry matter intake was higher (P = 0.046) for steers fed at 1.0% of BW of the PP silage-based concentrate than for steers fed at 1.75% or 2.5% of BW of the concentrate. The feeding levels of the PP silage-based concentrate had no effect on DM and nutrients digestibility, except for crude protein (CP) digestibility. CP digestibility tended to be lower (P = 0.071) for steers fed at 1.75% of BW of the PP silage-based concentrate than for steers fed at 1.0% or 2.5% of BW of the concentrate. The feeding levels of the PP silage-based concentrate also did not affect the in situ degradation parameter of hay and PP silage. The feeding levels of the PP silage-based concentrate did not affect ruminal pH, NH3-N and total VFA concentrations. The molar proportion of acetate was highest for steers fed at 1.0% of BW of the concentrate. In conclusion, in the urea-treated PP silage-based concentrate, CGM seems to be more effective than SBM for stabilizing the ruminal NH3-N concentration and to be advantageous for fiber digestion in the rumen. The feeding levels of the PP silage-based concentrate did not change the amount of VFA production in the rumen and the DM digestibility. Science †Potato pulp was ensiled for 6 months with urea (0.5%, as fed-basis) and beet pulp (9%, as-fed basis) before it was fed to the steers.
M. SUGIMOTO et al.Animal Science Journal (2008) 79, 443-452 †Potato pulp silage was ensiled for 6 months with urea (0.5%, as fed-basis) and beet pulp (9%, as-fed basis) before it was fed to the steers.
PROTEIN SOURCES WITH UREA-TREATED POTATO PULP 445No supplementation level ¥ diet interactions were detected (P Ն 0.05). ADF, acid detergent fiber; CGM, corn gluten meal; CP crude protein; DM, dry matter; EE, ether extract; NDF, neutral detergent fiber; OM, organic matter; SBM, soybean meal; s.e., standar...