Estimation of genetic diversity among 34 genotypes in the genus Cajanus with contrasting host response to the pod borer and its allied pests

Sahu, Alok Ranjan; Mishra, Rabindra Kishore; Panigrahi, Jogeswar

doi:10.1007/s12892-015-0045-5

Cited by 5 publications

(3 citation statements)

References 44 publications

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“…However, pigeonpea wild relatives have shown high levels of resistance to H. armigera [ 5 , 9 ]. The two most H. armigera -resistant wild pigeonpea species are Cajanus scarabaeoides and Cajanus cinereus [ 5 , 10 ]. Despite their poor agronomic characteristics, the pigeonpea wild relatives possess great potential as a source of new genes and markers for enhancing the resistance to biotic stress based on their high genetic diversity, including pest and disease resistance traits [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis Delineates the Transcriptional Resistance Mechanisms for Pod Borer Resistance in the Pigeonpea Wild Relative Cajanus scarabaeoides (L.) Thouars

Njaci

Ngugi-Dawit

Oduor

et al. 2020

IJMS

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Insect pests pose a serious threat to global food production. Pod borer (Helicoverpa armigera (Hübner)) is one of the most destructive pests of leguminous crops. The use of host resistance has been an effective, environmentally friendly and sustainable approach for controlling several agricultural pests. The exploitation of natural variations in crop wild relatives could yield pest-resistant crop varieties. In this study, we used a high-throughput transcriptome profiling approach to investigate the defense mechanisms of susceptible cultivated and tolerant wild pigeonpea genotypes against H. armigera infestation. The wild genotype displayed elevated pest-induced gene expression, including the enhanced induction of phytohormone and calcium/calmodulin signaling, transcription factors, plant volatiles and secondary metabolite genes compared to the cultivated control. The biosynthetic and regulatory processes associated with flavonoids, terpenes and glucosinolate secondary metabolites showed higher accumulations in the wild genotype, suggesting the existence of distinct tolerance mechanisms. This study provides insights into the molecular mechanisms underlying insect resistance in the wild pigeonpea genotype. This information highlights the indispensable role of crop wild relatives as a source of crucial genetic resources that could be important in devising strategies for crop improvement with enhanced pest resistance.

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Section: Introductionmentioning

confidence: 99%

Comparative Analysis Delineates the Transcriptional Resistance Mechanisms for Pod Borer Resistance in the Pigeonpea Wild Relative Cajanus scarabaeoides (L.) Thouars

Njaci

Ngugi-Dawit

Oduor

et al. 2020

IJMS

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“…scarabaeoides further revealed that out of eleven bivalents, eight were homologues which again indicated the similarities among these two species resulting in higher pollen germination as compared to C. platycarpus (Pundir and Singh, 1984). The use of molecular markers also suggested that large genetic differences occur between C. cajan and C. platycarpus (Sahu et al 2016). Very low pollen germination in crosses involving Pusa 992 and PADT-16 with ICP-15663 suggested that these species have distant lineage from their original ancestor and are reproductively isolated from each other.…”

Section: Discussionmentioning

confidence: 99%

“…Simple Sequence Repeat (SSR) markers are PCR based markers that are polymorphic, co-dominant, reliable, reproducible and abundant throughout the genome and have been used in assessment of genetic diversity in pigeonpea. SSRs are being effectively used by several workers to analyses genetic diversity in pigeonpea (Sahu et al 2016;Sharma et al 2020). To identify the pre fertilization barriers among the cultivated and wild pigeonpea, the present investigation was conducted to understand the exact nature of pre-fertilization barriers and molecular genetic diversity between different species so that depending on type of barriers effective techniques can be developed to overcome these barriers for introgression the desirable gene from wild to cultivated types.…”

Section: Introductionmentioning

confidence: 99%

Studies on pre-fertilization crossability barriers between cultivated and wild species of pigeonpea [Cajanus cajan (L.) Millspaugh]

Verma

Karn

Kumar

et al. 2023

Preprint

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The present day commercially grown cultivars of pigeonpea are susceptible to pod borer, pod fly insect pest and a number of diseases those causes huge economic loss every year to the farmers. On the other hand wild relative of cultivated pigeonpea possess genes for insect pest/ diseases resistance besides having some other useful genes. Transfer of desirable gene from wild to cultivated pigeonpea is hindered by several pre fertilization barriers. The identification of pre fertilization barriers will help in the introgression of desirable genes for insect pest and diseases resistance into cultivated types for enhancing and stabilizing pigeonpea production. In present investigation crossability barriers between the cultivated Cajanus cajan (PADT-16 and Pusa-992) and wild species Cajanus scarabaeoides (ICP-15683, ICP-15703) and Cajanus platycarpus (ICP-15663) were examined by pre-fertilization parameters like pollen germination, pollen tube growth, and pollen tube abnormalities. In hand-selfing and intervarietal crosses mean pollen germination and pollen tube growth was higher than interspecific crosses. Strong pre-fertilization barriers were observed in C. cajan x C. platycarpus crosses. However in interspecific crosses involving C. platycarpus as female, low level of reproductive barriers were observed when crossed with C. scarabeoides compared to cross with C. cajan, though pollen tube reached ovule in both crosses. More pollen tube abnormalities were observed in interspecific crosses involving cultivated and species from tertiary gene pool. Molecular diversity among experimental material through SSR markers exhibited maximum diversity between cultivated C. cajan and wild C. platycarpus whereas closest relationship was observed between C.scarabeoides ICP-15683 and ICP-15703.

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Novel biotechnological strategies to combat biotic stresses: polygalacturonase inhibitor (PGIP) proteins as a promising comprehensive option

Rathinam

Rao

Sreevathsa

2020

Appl Microbiol Biotechnol

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Estimation of genetic diversity among 34 genotypes in the genus Cajanus with contrasting host response to the pod borer and its allied pests

Cited by 5 publications

References 44 publications

Comparative Analysis Delineates the Transcriptional Resistance Mechanisms for Pod Borer Resistance in the Pigeonpea Wild Relative Cajanus scarabaeoides (L.) Thouars

Comparative Analysis Delineates the Transcriptional Resistance Mechanisms for Pod Borer Resistance in the Pigeonpea Wild Relative Cajanus scarabaeoides (L.) Thouars

Studies on pre-fertilization crossability barriers between cultivated and wild species of pigeonpea [Cajanus cajan (L.) Millspaugh]

Novel biotechnological strategies to combat biotic stresses: polygalacturonase inhibitor (PGIP) proteins as a promising comprehensive option

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