Estimation of Growth Fraction in Fine Needle Aspirates from Non-Hodgkin’s Lymphoma Using Anti–Proliferating Cell Nuclear Antigen

Cytomorphologic spectrum of small lymphocytic lymphoma in patients with an accelerated clinical course

“…27 These percentages of PCNA expression in each grade of lymphoma are comparable to those of Ki-67 expression in our study, in which TLCL had a mean value of 48.1%, and SLL had a mean value of 11.2%. P53 immunostaining also has been used to identify transformed large cell lymphoma; however, the results have been more variable.…”

“…PCNA expression also was studied previously 21,27 : The average expression of PCNA was highest (46%; range, 9 -86%) in patients with large cell lymphoma and lowest (7%) in patients with SLL in one study, 21 and it was 74% in patients with high-grade lymphoma and 17.4% in patients with low-grade lymphoma in another study. 27 These percentages of PCNA expression in each grade of lymphoma are comparable to those of Ki-67 expression in our study, in which TLCL had a mean value of 48.1%, and SLL had a mean value of 11.2%.…”

“…21 Others have studied the utility of employing the Ki-67 proliferation rate or the expression of proliferating cell nuclear antigen (PCNA) to detect lymphoma cells that are proliferating. [23][24][25][26][27] A study by Katz et al reported that the Ki-67 positive labeling index quantitated on a single cytospin slide, as measured by visual scoring or image analysis, was correlated positively with increasing grade of lymphoma according to the Kiel classification and the Working Formulation. 23 A similar correlation of Ki-67 expression with increasing grade lymphoma or poor clinical outcome has been confirmed in studies using FNA specimens 24,25 and in a prospective, multiinstitutional cooperative group trial using snap-frozen tissue sections.…”

Apoptotic Index from Fine Needle Aspiration Cytology as a Criterion To Predict Histologic Grade of Non-Hodgkin’s Lymphoma

“…5,16 Indeed, rates of cellular proliferation in FNAs from NHL also relate to histologic tumor grade. 23,25 Cellular kinetics (such as proliferation and/or apoptosis) could conceivably provide objective information on NHLs in addition to morphologic classification. 16,20 Such variables are suitable for quantitation from FNA samples, provided that the diagnosis of NHL is established first.…”

Grading Follicular Lymphoma on Fine Needle Aspiration Specimens

“…[35][36][37] It is well known that small centrocytes proliferate very slowly but are more likely to dissemi-…”