Estimation of Haemoglobin by the Alkaline Haematin Method

Clegg, J. W.; King, E. J.

doi:10.1136/bmj.2.4263.329

Cited by 120 publications

(30 citation statements)

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“…Briefly, the activity of blood δ-aminolevulinic acid dehydratase (ALAD) was assayed according to the procedure of Berlin and Schaller (1974), whereas blood haemoglobin was determined by the method of Clegg and King (1942) using the cyanomethaemoglobin technique in a Systronic digital Hb meter, model 185. Zinc protoporphyrin (ZPP) was determined in a drop of blood with the help of a haematofluorimeter (Model 2060, Aviv, Lakewood, USA) and a calibrated glass slide (Grandjean, 1979).…”

Section: Biochemical Assaysmentioning

confidence: 99%

Haematological, hepatic and renal alterations after repeated oral and intraperitoneal administration of monoisoamyl DMSA. II. Changes in female rats

Flora

Mehta

2003

J of Applied Toxicology

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We recently reported the effects of repeated administration of the monoisoamyl ester of dimercaptosuccinic acid (MiADMSA) on a few selected biochemical variables indicative of haematopoietic, liver, kidney and brain toxicity, oxidative stress and essential metal status in male rats. The present investigation studies similar changes in female rats to fi nd out if the changes are independent of gender. The results suggest significant and pronounced toxic effects of MiADMSA on haem biosynthesis, liver and kidneys in female rats exposed to higher doses of orally (p.o.) or intraperitoneally (i.p.) administered MiADMSA, compared with the effects in male rats. No effects on brain tissues were seen. A pronounced depletion of copper was noted in the blood and liver of MiADMSA administered rats, irrespective of route of exposure. It can be concluded that the administration of MiADMSA in female rats is confounded with side-effects and may require caution during its use and further exploration.

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Section: Biochemical Assaysmentioning

confidence: 99%

Haematological, hepatic and renal alterations after repeated oral and intraperitoneal administration of monoisoamyl DMSA. II. Changes in female rats

Flora

Mehta

2003

J of Applied Toxicology

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“…The hemoglobin concentration of the peripheral blood was determined by the alkaline hematin method (23).…”

Section: Methodsmentioning

confidence: 99%

Positive Direct Coombs Test Induced by Phenylhydrazine1

Muirhead¹,

Groves²,

Bryan³

1954

J. Clin. Invest.

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“…Determination of Haemoglobin.-.Haemoglobin was measured as alkaline haematin in the photoelectric colorimeter; the method employed was essentially that of Wu (1922) as used by Clegg and King (1942 King (1937) adapted for the photoelectric colorimeter (King, 1947) was used, with the important modification (King and Wootton, 1948) of diluting the plasma with buffer if the readings were found or expected to exceed one and a half times the standard. This modification leads to considerably higher readings than those given by the original method and no doubt goes a long way towards meeting criticisms of the method (e.g., Gray and Whidborne, 1946).…”

Section: Clinical Materialsmentioning

confidence: 99%