Estimation of intracellular calcium activity in confluent monolayers of primary cultures of quail medullary bone osteoclasts

Abstract

SUMMARYThe present study was concerned with an investigation of the relative utilities of the fluorescent indicators fura-2 and fluo-3 for spectrofluorimetric estimation of the intracellular calcium concentration ([Ca2"] in a similar manner to mammalian osteoclasts when they are removed from the bone microenvironment, but retain a refractoriness to CT under these conditions.