Estimation of l-α-[2-3H]acetylmethadol in biological materials and its separation from some metabolites and congeners on glass fibre sheets

Misra, Aditi; Bloch, R; Mulé, S. J.

doi:10.1016/s0021-9673(01)81061-x

Cited by 5 publications

(3 citation statements)

References 7 publications

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“…It is of interest that Kaiko et al (11) recovered 91% of NNAM from plasma and Kuttab et al (14) recovered 96% of NNAM from urine. The solvent, pH and samples used by these in vestigators (11,14) were not much different from those used by Misra et al ( 12): however, the methods of identification of NNAM were different. According to our experience, the organic extraction recovery of NNAM from biological samples was a function of time when NNAM was measured by GLC.…”

Section: Resultsmentioning

confidence: 88%

“…The recovery of NNAM from liver tissue is significantly higher than tire recovery of 4-9% reported by Misraetal. (12). It is of interest that Kaiko et al (11) recovered 91% of NNAM from plasma and Kuttab et al (14) recovered 96% of NNAM from urine.…”

Section: Resultsmentioning

confidence: 99%

“…Misra el al. (12) developed a method using glass fiber silica gel impregnated sheets for the separation of LAAM and its metabolites. However, the method has a ten dency to overload when working with biologi cal extracts (13), and the recovery of NNAM was very low.…”

Section: Introductionmentioning

confidence: 99%

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Isotope Dilution Technique for the Estimation of l-Alpha-[2-3H]-Acetylmethadol and Its Metabolites in Biological Samples

Man¹,

Harris²,

Woods³

et al. 1980

Pharmacology

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An isotope dilution technique for the determination of l-α-(2-³H)-acetylmethadol and its metabolites from biological samples is described. The parent drug and metabolites were extracted from biological samples with chlorobutane after the addition of unlabeled internal standards. The extracts were purified by two-dimensional thin-layer chromatography for quantification by gas-liquid chromatography and scintillation counting. Dinoracetylmethadol (NNAM) was found to be converted to 6-acetamido-4,4-diphenyl-3-heρtanol (AMIDE) during the extraction procedure. The conversion of NNAM to AMIDE was confirmed by gas chromatography-mass spectrometry.

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