2015
DOI: 10.4315/0362-028x.jfp-14-261
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Estimation of Listeria monocytogenes and Escherichia coli O157:H7 Prevalence and Levels in Naturally Contaminated Rocket and Cucumber Samples by Deterministic and Stochastic Approaches

Abstract: The aims of the present study were to determine the prevalence and levels of Listeria monocytogenes and Escherichia coli O157:H7 in rocket and cucumber samples by deterministic (estimation of a single value) and stochastic (estimation of a range of values) approaches. In parallel, the chromogenic media commonly used for the recovery of these microorganisms were evaluated and compared, and the efficiency of an enzyme-linked immunosorbent assay (ELISA)-based protocol was validated. L. monocytogenes and E. coli O… Show more

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Cited by 9 publications
(10 citation statements)
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“…In the present study, ALOA and RAPID'L.mono exhibited identical performance. This was not the case in previous studies [19,23,29] and thus it may be characterized as unusual and could probably be assigned to the rather restricted amount of samples examined in the present study. On the contrary, Fluorocult E. coli O157:H7 and CT-SMAC performance revealed their weaknesses.…”
Section: Discussioncontrasting
confidence: 64%
See 2 more Smart Citations
“…In the present study, ALOA and RAPID'L.mono exhibited identical performance. This was not the case in previous studies [19,23,29] and thus it may be characterized as unusual and could probably be assigned to the rather restricted amount of samples examined in the present study. On the contrary, Fluorocult E. coli O157:H7 and CT-SMAC performance revealed their weaknesses.…”
Section: Discussioncontrasting
confidence: 64%
“…In total, 10 cases were reported for this period [15,17,18]. Regarding their prevalence in fruits and vegetables in Greece, it has been estimated at 6% and 3% in cucumber for L. monocytogenes and E. coli O157:H7, respectively and at 7% in rocket for both pathogens [19].…”
Section: Introductionmentioning
confidence: 99%
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“…In brief, cell-free supernatant was obtained from overnight bacterial cultures, neutralized, treated with catalase, and added to the wells that were punched in freshly prepared lawns containing a mixture of 5 strains of L. monocytogenes, E. coli O157:H7 and Salmonella sp. [11,12]. Growth inhibition exceeding 5 mm around the wells was used as an indicator of antimicrobial activity of the supernatant applied.…”
Section: Antimicrobial Activitymentioning
confidence: 99%
“…Fresh vegetables with increased incidence of L. monocytogenes are lettuce, cabbage, celery, cucumber, corn, tomato, and melon, some of these being involved in serious listeriosis outbreaks over time (Beuchat, 2002). L. monocytogenes prevalence in naturally contaminated cucumbers is 6%, the contamination level ranging from 0.04 to 0.170 CFU/25 g sample (Hadjilouka et al, 2015). Although stored under refrigeration conditions, cucumbers can provide optimal conditions for L. mononocytogenes proliferation.…”
Section: Introductionmentioning
confidence: 99%