Estimation of retina thermal response in photochemical damage studies

Oian, Chad A.; Zhang, Jie; Thomas, Robert J.; Hunter, Jennifer J.

doi:10.2351/1.5056869

Cited by 2 publications

(1 citation statement)

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“…The temperature rise during exposure was calculated using a thermal model to be less than 1 K at all tested wavelengths. 27 In addition, photothermal damage appears right after the exposure, however, we did not observe any changes in the immediate post-exposure images beyond the expected AF reduction. 13 Photochemical damage is expected to be confined to the illumination field.…”

Section: Discussionmentioning

confidence: 50%

Spectral Dependence of Light Exposure on Retinal Pigment Epithelium Disruption in Living Primate Retina

Zhang,

Sabarinathan,

Bubel

et al. 2024

Invest. Ophthalmol. Vis. Sci.

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Purpose RPE disruption with light exposures below or close to the American National Standards Institute (ANSI) photochemical maximum permissible exposure (MPE) have been observed, but these findings were limited to two wavelengths. We have extended the measurements across the visible spectrum. Methods Retinal imaging with fluorescence adaptive optics scanning light ophthalmoscopy (FAOSLO) was used to provide an in vivo measure of RPE disruption at a cellular level. The threshold retinal radiant exposures (RREs) for RPE disruption (localized detectable change in the fluorescence image) were determined at 460, 476, 488, 530, 543, 561, 594, 632, and 671 nm (uniform 0.5° square exposure) using multiples locations in 4 macaques. Results FAOSLO is sensitive in detecting RPE disruption. The visible light action spectrum dependence for RPE disruption with continuous wave (CW) extended field exposures was determined. It has a shallower slope than the current ANSI blue-light hazard MPE. At all wavelengths beyond 530 nm, the disruption threshold is below the ANSI blue-light hazard MPE. There is reciprocity of exposure irradiance and duration for exposures at 460 and 594 nm. Conclusions We measured with FAOSLO the action spectrum dependence for photochemical RPE disruption across the visible light spectrum. Using this in vivo measure of phototoxicity provided by FAOSLO, we find that thresholds are lower than previously measured. The wavelength dependence in our data is considerably shallower than the spectral dependence of the traditional ANSI blue-light hazard, emphasizing the need for more caution with increasing wavelength than expected.

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Section: Discussionmentioning

confidence: 50%