ESTIMATION OF SERUM Vicfflb LIGHT CHAINS IN RHEUMATOID ARTHRITIS AND CORRELATION WITH CD5-POSITIVE -CELLS

Smalley, Helen B.; Todd, J A; Barnes, R. M. R.; Thompson, R. N.; Abraham, George N.; Johnson, Peter

doi:10.1093/rheumatology/29.5.325

Cited by 5 publications

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“…We found increased percentages of CD5 B cells in RA (fig 4), however, these differences were not significant compared with healthy controls (table 1). The increased frequency of CD5 B cells in RA blood was initially reported by Plater-Zyberk et al, in 1985's and observed by others, 9 15 17 32 but not all reports have confirmed these findings.33 34 These controversial results may be due to a number of reasons, including the wide differences in the epitope density of CD5 on the B cell membrane, patient sampling, drug regime, and the different sensitivities of the methods used. Our study found different epitope density of CD5 on the B cells with three populations of CD5 B cells being observed ( fig 5) Our data showed the levels of CD5 B cells in the PB of patients with RA and other inflammatory arthritides were stable over time (table 2).…”

Section: Discussionmentioning

confidence: 91%

Low molecular weight IgM and CD5 B lymphocytes in rheumatoid arthritis.

Geddes²,

Roberts‐Thomson³

1994

Annals of the Rheumatic Diseases

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Section: Discussionmentioning

confidence: 91%

Low molecular weight IgM and CD5 B lymphocytes in rheumatoid arthritis.

Geddes²,

Roberts‐Thomson³

1994

Annals of the Rheumatic Diseases

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The B lymphocyte in rheumatoid arthritis: analysis of rearranged Vx genes from B cells infiltrating the synovial membrane

et al. 1995

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The participation of the humoral immune system in rheumatoid arthritis (RA) is characterized by the production of rheumatoid factors (RF). RF are autoantibodies against the Fc part of IgG which are encoded by diverse germ-line genes. Most of the RF-encoding genes are unmutated, but in RA, a substantial quantity is encoded by somatically mutated genes. In addition, the synovial membranes (SM) of the diseased joints of RA patients are infiltrated by B lymphocytes which form germinal center-like aggregates. To analyze the local immune response, B cell foci from two RA SM were isolated by micromanipulation. From DNA of these foci, the rearranged kappa light chain variable region (V kappa) genes were amplified by polymerase chain reaction (PCR), cloned and sequenced. The amplification of different V kappa-J kappa combinations of different foci suggested oligoclonal expansion of B lymphocytes, which was confirmed by sequence analysis: each PCR product contained members of a single B cell clone. The sequence analysis of 29 different clones revealed rearrangements of diverse V kappa genes. Both frequent representatives of the V kappa 3 and the V kappa 1 family, as well as rarely used genes such as the L10 and B2 genes of the V kappa 2 and V kappa 5 families were found. Of the eleven potentially functional gene rearrangements, eight were significantly mutated, indicating their derivation from antigen-selected B cells. Intraclonal diversity in one of these clones may suggest ongoing mutation in the diseased synovial membrane of patients with RA.

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The study of circulating CD5 positive B lymphocytes in Chinese patients with rheumatoid arthritis

et al. 1996

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Circulating CD5+ B lymphocytes were studied in 39 Chinese patients with rheumatoid arthritis (RA). Blood cells were stained with anti-CD5 and anti-CD19 monoclonal antibodies and were analyzed by flow cytometry. The results showed that there was no elevation of CD5+ B cells in RA patients when compared with 41 healthy control subjects. The circulating levels of CD5+ B cell correlated neither with serum titers of rheumatoid factor (RF) nor with disease activities in these patients. The CD5+ B cell levels remained relatively constant after a serial follow-up for 12 months. The similar pattern of epitope density of CD5 antigens also existed in the same patients. Although most studies in Caucasians revealed increased levels of CD5+ B cells in RA patients, measurements of this marker were not useful for the evaluation of disease activities in Chinese patients. Levels of CD5+ B cells may reflect more individual genetic background and may play a minor role in the flare-up of activities in Chinese patients with RA.

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ESTIMATION OF SERUM Vicfflb LIGHT CHAINS IN RHEUMATOID ARTHRITIS AND CORRELATION WITH CD5-POSITIVE -CELLS

Cited by 5 publications

References 0 publications

Low molecular weight IgM and CD5 B lymphocytes in rheumatoid arthritis.

Low molecular weight IgM and CD5 B lymphocytes in rheumatoid arthritis.

The B lymphocyte in rheumatoid arthritis: analysis of rearranged Vx genes from B cells infiltrating the synovial membrane

The study of circulating CD5 positive B lymphocytes in Chinese patients with rheumatoid arthritis

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