> We thank reviewer 1 for the detailed reading and constructive feedback, which will help to sharpen our manuscript. We quote hereafter the original comments (italic), followed by our reply (normal font) on how we plan to modify our manuscript. In this study, the authors proposed to analyze eDNA from water samples to identify water source in an alpine catchment. For this, they collected water at 11 sampling dates (March-August 2017) in 10 stream sites, located in springs, tributaries and along the main channel, as defined by the authors. At each sampling date and site, they also measured temperature, conductivity, and the stable isotopes of water. Their main objective was to identify the spatial variability of water sources within the catchment,