Estimation of surface potential of lymphocytes from patients with leukemia using Kelvin probe mode

Abstract

Abstract-The feasibilities of the Kelvin probe mode were shown to estimate a surface potential of lympho cytes from the patients with leukemia. Having compared the values of the surface potential obtained by the traditional method of microelectrophoresis and by the Kelvin probe mode, it was established that the use of the atomic force microscope made it possible to obtain data which were of specified diagnostic importance while studying lymphocyte functional status at different stages in leucopoiesis and indicated progression of the pathological process.

Changes of the Biophysical Properties of Blood Corpuscles from the Elderly under Mechanical Stress in vitro

“…The electrical properties of the erythrocyte, granulocyte, lymphocyte, and platelet membranes were assessed by measuring the surface potentials with the AFM in the Kelvin probe mode. The preparation of the cell suspension for measuring the surface potential and the measurement followed the method reported in [11]. Use was made of cantilevers with conducting titanium coating of the NSG03/TiN series (Nanoworld, United States).…”

Structure and Properties of Lymphocytes’ Surfaces in Patients With Chronic Lymphoblastic Leucosis

“…Suspended mixture of lymphocytes for measuring surface potential was prepared according to method [3]. Measuring of SP was performed with the usage of cantilever with titanium current conducting coating of NSG03/TiN series (Nanoworld, USA).…”

Application of Technologies of Atomic Force Microscope Investigation for Evaluation of Structure and Properties of Blood Cells’ Surfaces

“…Study of elastic properties of blastic forms of blood was performed at atomic force microscope INTEGRA Vita (configuration on the base of inverted optical microscope OlympusIX-71, produced by NTMDT, Zelenograd, 2009 Suspension of cells for measuring surface potential (SP) were prepared according to the method, described above [3]. Measuring of SP was performed with the help of cantilever with electrically conducting titanium coating of NSG03/TiN series (Nanoworld, USA).…”