Estimation of vitamin D3 and 25-hydroxyvitamin D3 in muscle and adipose tissue of rats and man

Lawson, David; Douglas, Jeffrey A.; Lean, Michael E. J.; Sedrani, Saleh H.

doi:10.1016/0009-8981(86)90223-8

Cited by 55 publications

(46 citation statements)

References 12 publications

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“…Our results regarding the placebo group with a median subcutaneous vitamin D 3 concentration of 32 ng/g is quite similar to that reported by other groups (7,13,14,15) (Table 3). In all of these studies, there was a great interindividual variability in the fat vitamin D 3 concentration as exemplified in our study by the lowest concentration of 3.6 ng/g and the highest concentration of 118 ng/g in the placebo group, and of 89 and 510 ng/g in the vitamin D group.…”

Section: Discussionsupporting

confidence: 91%

Vitamin D3 increases in abdominal subcutaneous fat tissue after supplementation with vitamin D3

Didriksen¹,

Burild²,

Jakobsen³

et al. 2015

European Journal of Endocrinology

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Section: Discussionsupporting

confidence: 91%

Vitamin D3 increases in abdominal subcutaneous fat tissue after supplementation with vitamin D3

Didriksen¹,

Burild²,

Jakobsen³

et al. 2015

European Journal of Endocrinology

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“…This sequestration theory is probably the most supported within the literature and others have since quantified that obese children and adults require 2-5 times more vitamin D to prevent or treat deficiency compared with their lean counterparts, because of such sequestration in adipose tissue (27) . The cholecalciferol content of adipose tissue has been quantified (28)(29)(30) and is positively correlated with serum 25(OH)D concentrations in obese subjects (29) . In a recent pilot study of six females, a significantly lower cholecalciferol and 25(OH)D (but not 1,25(OH)D) content of subcutaneous adipose tissue was observed in obese compared with lean individuals (30) .…”

Section: Commonly Suggested Mechanismsmentioning

confidence: 99%

Vitamin D and obesity: current perspectives and future directions

2014

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In recent years, new functional roles of vitamin D beyond its traditional role in calcium homoeostasis and bone metabolism have emerged linking the fat-soluble vitamin to various non-communicable diseases. Vitamin D deficiency (25-hydroxyvitamin D (25(OH)D) < 25-30 nmol/l) and sub-optimal status (25(OH)D < 50-100 nmol/l) are increasingly associated with unfavourable metabolic phenotypes, including insulin resistance, type 2 diabetes and CVD; conditions also commonly linked with overweight and obesity. Early studies reported poor vitamin D status in the morbidly obese. More recently, it has been observed that a graded relationship between vitamin D status and BMI, or specifically adiposity, exists in the general population. A number of hypotheses have been proposed to explain the potential mechanisms whereby alterations in the vitamin D endocrine system occur in the obese state. Plausible explanations include sequestration in adipose tissue, volumetric dilution or negative feedback mechanisms from increased circulating 1,25-dihydroxyvitamin D 3 . Others hypothesise that heavier individuals may partake in less outdoor activity, may also cover-up and wear more clothing than leaner individuals, thus decreasing sun exposure and limiting endogenous production of cholecalciferol in the skin. Moreover, in some but not all studies, BMI and adiposity have been negatively associated with the change in vitamin D status following vitamin D supplementation. It therefore remains unclear if body size and/or adiposity should be taken into account when determining the dietary requirements for vitamin D. This review will evaluate the current evidence linking vitamin D status and supplementation to overweight and obesity, and discuss the implications for setting dietary requirements. Vitamin D: Obesity: BMI: Fat mass: Adipose tissueTraditional roles of vitamin D lie within the musculoskeletal system, maintaining calcium homoeostasis and bone metabolism, with deficiency leading to conditions such as rickets in children, or osteoporosis and osteomalacia in adults (1) . However, obesity is now frequently cited as a cause of vitamin D deficiency (2,3) , which may in fact lead to other co-morbidities, such as diabetes and CVD, conditions commonly linked to obesity (4,5) . Indeed, obesity and vitamin D deficiency have concomitantly reached epidemic levels worldwide and research linking the two has grown extensively over the last number of years. It is reasonable to suggest that this nutritional inadequacy may be driven by the increasing obesity rates within the general population, and may therefore improve with a reversal of the latter. However, despite the plethora of evidence available, the mechanisms remain largely unclear and the area of reverse causality is also commonly debated within the literature: does obesity lead to vitamin D deficiency or vice versa?Vitamin D is not technically a vitamin, but is better classified as an active circulating pre-hormone within the body. Limited amounts of vitamin D are obtained from dietary sourc...

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“…Prior studies have reported fat tissue to be a major repository in the body for vitamin D, but the studies performed in humans have only examined vitamin D concentrations in fat tissue of amputated limbs and in cadavers [1,2]. In subjects given intravenous injections of radioactively labeled vitamin D 3 , subsequent fat tissue samples obtained either from amputated limbs or at autopsy contained higher levels of the radioactive dose compared to other tissue types [1].…”

Section: Introductionmentioning

confidence: 96%

Vitamin D3 in fat tissue

Blum

Dolnikowski

Seyoum

et al. 2008

Endocr

339

235

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The literature describing vitamin D content of fat tissue is extremely limited. We conducted a pilot study that measured the concentrations of vitamin D(3) in the fat tissue and serum of obese adults. These measurements were performed using a new liquid chromatography mass spectrometry (LC/MS) method. The objectives of this study were: to measure and report the vitamin D(3) concentration in serum and subcutaneous fat samples from obese individuals and to examine the association of vitamin D(3) in fat with vitamin D(3) in serum. This cross-sectional study was conducted in 17 obese men and women who were scheduled to undergo gastric bypass surgery. The mean vitamin D(3) concentration in subjects' subcutaneous fat tissue samples was 102.8 +/- 42.0 nmol/kg. The mean vitamin D(3) concentration in serum was 7.78 +/- 3.99 nmol/l. Vitamin D(3) concentrations of fat tissue and serum were positively correlated (r = 0.68, P = 0.003). Consistent with previous findings in obese subjects, subjects in this study had suboptimal vitamin D status as demonstrated by a mean 25-hydroxyvitamin D concentration of 43.3 +/- 15.4 nmol/l. In conclusion, fat tissue vitamin D(3) can be measured by LC/MS and is detectable in obese subjects with suboptimal vitamin D status. Compatible with the long-standing concept that fat tissue is a storage site for vitamin D, fat tissue and serum vitamin D(3) concentrations were positively correlated.

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Estimation of vitamin D3 and 25-hydroxyvitamin D3 in muscle and adipose tissue of rats and man

Cited by 55 publications

References 12 publications

Vitamin D3 increases in abdominal subcutaneous fat tissue after supplementation with vitamin D3

Vitamin D3 increases in abdominal subcutaneous fat tissue after supplementation with vitamin D3

Vitamin D and obesity: current perspectives and future directions

Vitamin D3 in fat tissue

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