Estradiol-17β Regulates Expression of Luteal DNA Methyltransferases and Genes Involved in the Porcine Corpus Luteum Function In Vivo

Kaczyński, Piotr; Baryla, Monika; Goryszewska, Ewelina; Wacławik, Agnieszka

doi:10.3390/ijms22073655

Cited by 7 publications

(5 citation statements)

References 55 publications

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“…Our findings are also consistent with a report indicating that the expression of DNMTs in the human endometrium decreased during the secretory phase and was reduced by E 2 and P4 in endometrial stromal cells [ 42 ]. Similarly, our recent findings indicate that E 2 controlled DNMT expression in the porcine corpus luteum in vivo and changes in their abundance were significant during pregnancy [ 31 ]. Interestingly, recent studies have indicated that E 2 can affect the expression of DNMTs via a complex mechanism involving a number of co-regulators [ 43 ].…”

Section: Discussionmentioning

confidence: 71%

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Novel role for conceptus signals in mRNA expression regulation by DNA methylation in porcine endometrium during early pregnancy

Kaczyński

Weijden

Goryszewska-Szczurek

et al. 2022

Biology of Reproduction

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During early pregnancy, porcine conceptuses (the embryos with associated membranes) secrete estradiol-17β (E2)—their major signal for maternal recognition of pregnancy—and prostaglandin E2 (PGE2). Both hormones induce prominent changes of the endometrial transcriptome in vivo. Studies on endometrial pathologies have shown that E2 affects gene expression by epigenetic mechanisms related to DNA methylation. Herein, we determined the effects of E2 and PGE2 alone, and a combined E2 + PGE2 treatment administered into the uterine lumen in vivo on the expression and activity of DNA-methyltransferases (DNMT) and on CpG methylation patterns of selected genes in porcine endometrium. To compare the effect of treatment with the physiological effect of pregnancy, endometria from day 12 pregnant/cyclic gilts were included. Both E2 and PGE2 significantly reduced the expression of DNMTs. Likewise, the expressions of DNMT1 and DNMT3A were decreased on day 12 of pregnancy compared to the estrous cycle. DNMT activity increased in endometrial samples following E2 treatment and in gilts on day 12 of pregnancy. Treatment with E2 alone and/or simultaneously with PGE2 altered endometrial DNA methylation of CpG sites of ADAMTS20, ADH1C, BGN, PSAT1 and WNT5A. Different CpG methylation patterns of ADAMTS20, BGN, DMBT1, RASSF1 and WNT5A were found in the endometrium on day 12 of pregnancy compared to day 12 of the estrous cycle. Significant correlations were detected between CpG methylation and gene expression for ADAMTS20, ADH1C, BGN, DMBT1, PSAT1 and WNT5A. Our results indicate that CpG methylation induced by embryonic signals may contribute to regulating endometrial gene expression during pregnancy establishment.

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Section: Discussionmentioning

confidence: 71%

“…The endometrial DNMT1 , DNMT3A , and DNMT3B gene expression were determined using quantitative PCR (qPCR, real-time RT-PCR), as described previously [ 31 ]. Detailed information of qPCR procedure has been provided in Supplementary Information.…”

Section: Methodsmentioning

confidence: 99%

Novel role for conceptus signals in mRNA expression regulation by DNA methylation in porcine endometrium during early pregnancy

Kaczyński

Weijden

Goryszewska-Szczurek

et al. 2022

Biology of Reproduction

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“…The role of E2 in the luteal function in pigs is not clearly understood. Whereas some studies report its beneficial effect on CL function [ 45 , 46 ], others suggest E2 to play a role in CL regression [ 22 , 47 ]. Our results suggest that actions of E2 must be dependent upon the expression of E2-metabolizing enzymes, which differ in abundance during the estrous cycle.…”

Section: Discussionmentioning

confidence: 99%

Changes in Porcine Corpus Luteum Proteome Associated with Development, Maintenance, Regression, and Rescue during Estrous Cycle and Early Pregnancy

Likszo

Skarzynski

Jalali

2021

IJMS

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Corpus luteum (CL), a transitory gland, undergoes rapid growth in a limited time to produce progesterone (P4) followed by its regression. A complex molecular signaling is involved in controlling luteal P4 production. In the present study, 2D gel electrophoresis-based proteomics and in silico functional analysis were used to identify changes in key proteins and pathways in CL along the different stages of the estrous cycle as its development progresses from early (Day 3) to mid-luteal phase (Day 9), effective functioning (Day 12) followed by regression (Day 15) or, in the case of pregnancy, rescue of function (Day 15). A total of 273 proteins were identified by MALDI-MS/MS analysis that showed significant changes in abundances at different stages of CL development or regression and rescue. Functional annotation of differentially abundant proteins suggested enrichment of several important pathways and functions during CL development and function maintenance including cell survival, endocytosis, oxidative stress response, estradiol metabolism, and angiogenesis. On the other hand, differentially abundant proteins during CL regression were associated with decreased steroid synthesis and metabolism and increased apoptosis, necrosis, and infiltration of immune cells. Establishment of pregnancy rescues CL from regression by maintaining the expression of proteins that support steroidogenesis as pathways such as the super-pathway of cholesterol biosynthesis, RhoA signaling, and functions such as fatty acid metabolism and sterol transport were enriched in CL of pregnancy. In this study, some novel proteins were identified along CL development that advances our understanding of CL survival and steroidogenesis.

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“…The final 20-µL reaction volume comprised the TaqMan Gene Expression Master Mix and 50 ng of cDNA. Relative gene expression was normalized against the reference gene PPIA 7 , 46 (assay ID: Ss03394782_g1). The relative gene expression levels were determined by following the method outlined by Livak and Schmittgen 47 , using the comparative cycle threshold (2 -ΔΔCt ) approach.…”

Section: Methodsmentioning

confidence: 99%

In vitro effect of visfatin on endocrine functions of the porcine corpus luteum

Mlyczyńska,

Rytelewska,

Zaobidna

et al. 2024

Sci Rep

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Previously, we demonstrated the expression of visfatin in porcine reproductive tissues and its effect on pituitary endocrinology. The objective of this study was to examine the visfatin effect on the secretion of steroid (P4, E2) and prostaglandin (PGE2, PGF2α), the mRNA and protein abundance of steroidogenic markers (STAR, CYP11A1, HSD3B, CYP19A1), prostaglandin receptors (PTGER2, PTGFR), insulin receptor (INSR), and activity of kinases (MAPK/ERK1/2, AKT, AMPK) in the porcine corpus luteum. We noted that the visfatin effect strongly depends on the phase of the estrous cycle: on days 2–3 and 14–16 it reduced P4, while on days 10–12 it stimulated P4. Visfatin increased secretion of E2 on days 2–3, PGE2 on days 2–3 and 10–12, reduced PGF2α release on days 14–16, as well as stimulated the expression of steroidogenic markers on days 10–12 of the estrous cycle. Moreover, visfatin elevated PTGER mRNA expression and decreased its protein level, while we noted the opposite changes for PTGFR. Additionally, visfatin activated ERK1/2, AKT, and AMPK, while reduced INSR phosphorylation. Interestingly, after inhibition of INSR and signalling pathways visfatin action was abolished. These findings suggest a regulatory role of visfatin in the porcine corpus luteum.

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Estradiol-17β Regulates Expression of Luteal DNA Methyltransferases and Genes Involved in the Porcine Corpus Luteum Function In Vivo

Cited by 7 publications

References 55 publications

Novel role for conceptus signals in mRNA expression regulation by DNA methylation in porcine endometrium during early pregnancy

Novel role for conceptus signals in mRNA expression regulation by DNA methylation in porcine endometrium during early pregnancy

Changes in Porcine Corpus Luteum Proteome Associated with Development, Maintenance, Regression, and Rescue during Estrous Cycle and Early Pregnancy

In vitro effect of visfatin on endocrine functions of the porcine corpus luteum

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