Estradiol agonists inhibit human LoVo colorectal-cancer cell proliferation and migration through p53

Abstract

Direct ER treatment may prove to be an attractive alternative therapy in the treatment of human colorectal tumors in the future.

Population-based analysis of colorectal cancer risk after oophorectomy

“…The expression of ER‐β is different in proximal versus distal colon; it is low in tumour tissue compared with normal colonic mucosa and is associated inversely with stage of colonic cancer. In vitro , oestradiol and ER agonists exert an inhibitory effect on colorectal cancer cell growth. In rat and mouse models, oophorectomy stimulated colorectal carcinogenesis, whereas oestrogen exposure was protective.…”

Lentivirus-mediated silencing of USO1 inhibits cell proliferation and migration of human colon cancer cells

“…To carry out cell migration assay, we used a modified Boyden chamber (Corning) plate with 8-lm pore size polycarbonate membrane filters [15]. 1 9 10 4 of serumdeprived human colon HCT116 and HT 29 cells were added to the upper chamber, and the bottom chamber was filled with DMEM containing 20 % serum and incubated for 48 h. Then, 4 % paraformaldehyde was used to fix the cells on the membrane filter.…”

RPS24 knockdown inhibits colorectal cancer cell migration and proliferation in vitro

“…Ribosomal Protein (RP) is a ubiquitous RNA binding protein, the function of which is not only to participate jointly with ribosome RNA (rRNA) in the synthesis of protein, but also to take part in the regulation of gene transcription and the regulation of cell proliferation and apoptosis through its extra-ribosomal functions (4,5). The abnormal expression of RP often results in serious diseases such as anemia and tumor (6).…”

“…of a transwell chamber (Corning) plate with 8μm pore size polycarbonate membrane filters(5). Serum deprived human colon HTC116 and HT-29 cells 1×10 4 were added to the upper part of the Boyden chamber, and the bottom chamber was filled with DMEM containing 20% serum.…”