Estradiol modulation of hepatocyte growth factor by stromal fibroblasts in the female reproductive tract

Coleman, Kimberly D.; Wright, Jacqueline A.; Ghosh, Mimi; Wira, Charles R.; Fahey, John V.

doi:10.1016/j.fertnstert.2008.10.047

Cited by 25 publications

(27 citation statements)

References 29 publications

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“…Sugawara et al also identified HGF as a critical factor in E 2 -mediated regulation of human uterine ECs (Sugawara et al, 1997). E 2 treatment of human primary uterine stromal fibroblasts results in increased secretion of HGF (Coleman et al, 2009(Coleman et al, , 2012, which implies a functional relationship between E 2 and HGF. In addition to similarities between endogenous HGF and E 2 in normal FRT physiology, there is a correlation between reproductive tissue cancers and endometriosis and relatively high E 2 concentrations with increased HGF levels (Haslam and Woodward, 2003;Khan et al, 2005;Matsumoto and Nakamura, 2006).…”

Section: Figure 5 Effect Of Epithelial Cell (Ec) Co-culture With Stromentioning

confidence: 97%

“…In contrast, TNF-α and macrophage inhibitory protein (MIP)-3α/CCL20 secretion by uterine ECs is regulated indirectly by fibroblast cell secretion of growth factors such as hepatocyte growth factor (HGF) (Grant-Tschudy and Wira, 2005b). After E 2 treatment, uterine fibroblasts up-regulate the production of HGF, which in turn induces TNF-α and MIP-3α/CCL20 secretion (Grant-Tschudy and Wira, 2005b;Coleman et al, 2009;Haddad and Wira, 2010). E 2 can reduce the proinflammatory cytokine response after stimulation with pathogen ligands (via Toll-like receptors).…”

Section: Influence Of Sex Hormones On Cytokines Chemokines and Antimmentioning

confidence: 99%

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Endocrine Regulation of the Mucosal Immune System in the Female Reproductive Tract

et al. 2015

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Section: Figure 5 Effect Of Epithelial Cell (Ec) Co-culture With Stromentioning

confidence: 97%

Section: Influence Of Sex Hormones On Cytokines Chemokines and Antimmentioning

confidence: 99%

Endocrine Regulation of the Mucosal Immune System in the Female Reproductive Tract

et al. 2015

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“…Intriguingly, the withdrawal of sex hormones from uterine stromal fibroblasts treated with E 2 and P4 for several days in vitro, increases their secretion of proinflammatory cytokines including IL-6, CCL2, CXCL8 and IFNα amongst others 50 . Because epithelial-stromal cell interactions are essential in facilitating hormone-induced growth and development in the EM, we examined the effects of E 2 on hepatocyte growth factor (HGF) made by FRT fibroblasts and found that secretion is regulated by E 2 in the EM, but not in Cx and Ecx 51, 52 . Given that HGF regulates epithelial cell (EC) tight junction barrier integrity, these findings indicate that sex hormones mediate communication between fibroblasts and epithelial cells with potential consequences for HIV acquisition.…”

Section: Contribution Of Stromal Fibroblasts To the Frt Environmentmentioning

confidence: 99%

“…The mucosal immune system in the FRT contains a spectrum of immune cells including NK cells, neutrophils, macrophages and T cells that are different from their blood counterparts al 3,4,52, 53 . In addition to their unique phenotypic characteristics, these cells are functionally unique and responsive to changes in hormone balance during the menstrual cycle 5 .…”

Section: Introductionmentioning

confidence: 99%

The Role of Sex Hormones and the Tissue Environment in Immune Protection Against HIV in the Female Reproductive Tract

Wira

Rodriguez-García

Shen

et al. 2014

American J Rep Immunol

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Despite extensive studies of the mucosal immune system in the female reproductive tract (FRT) and its regulation by sex hormones, relatively little attention has been paid to the tissue environment in the FRT that regulates immune cell function. Consisting of secretions from epithelial cells, stromal fibroblasts and immune cells in tissues from the upper (Fallopian tubes, uterus and endocervix) and lower (ectocervix and vagina) tracts, each tissue compartment is unique and precisely regulates immune cells to optimize conditions for successful pregnancy and protection against sexually transmitted diseases including HIV. Our goal in this Review is to focus on the mucosal (tissue) environment in the upper and lower FRT. Specifically, this review will identify the contributions of epithelial cells and fibroblasts to the tissue environment and examine the impact of this environment on HIV-target cells. Much remains to be learned about the complex interactions with the tissue environment at different sites in the FRT and the ways in which they are regulated by sex hormones and chemical contraceptives. Awareness of the involvement of the tissue environment in determining immune cell function and HIV acquisition is crucial for the understanding the mechanisms that lead to HIV prevention, acquisition and the development of new therapeutic modalities of immune protection.

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“…Generally, the activation of a wide variety of ligands, including fibroblast growth factor, transforming growth factor-␤, epidermal growth factor, vascular endothelial growth factor, and hepatocyte growth factor (HGF) and its receptor MET, upregulates the expression of EMT-regulating transcription factors (26). Among these ligands, many actions of HGF are mirrored by estradiol in the female reproductive tract, and several investigators have reported a relationship between HGF and estradiol in the pathology of female genital disease (8,31,36).…”

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Estradiol-mediated hepatocyte growth factor is involved in the implantation of endometriotic cells via the mesothelial-to-mesenchymal transition in the peritoneum

Hayashi

Tanabe

Hayashi

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

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Ono YJ, Hayashi M, Tanabe A, Hayashi A, Kanemura M, Terai Y, Ohmichi M. Estradiol-mediated hepatocyte growth factor is involved in the implantation of endometriotic cells via the mesothelial-to-mesenchymal transition in the peritoneum. Am J Physiol Endocrinol Metab 308: E950 -E959, 2015. First published April 7, 2015 doi:10.1152/ajpendo.00573.2014.-The pathogenesis of endometriosis, a chronic painful gynecological disease characterized by the presence of endometrial tissue located outside of the uterus and often adhering to the peritoneum, is known to be estrogen dependent. However, the precise pathophysiology of endometriosis remains elusive. Recent studies indicate that the epithelial-to-mesenchymal transition (EMT) of human endometrial cells is important for the progression of endometriosis, and another previous study has implicated hepatocyte growth factor (HGF) in endometriosis progression. The aim of the present study was to examine the role of estradiol in the regulation of HGF production and progression of peritoneal endometriosis, focusing on the interactions between the peritoneum and endometriotic cells. Consequently, estradiol was found to promote the proliferation, invasion, and migration of immortalized human endometrial epithelial cells (hEECs) via HGF upregulation, and the estradiol-induced direct binding of estrogen receptor-␣ to the HGF promoter was confirmed on a chromatin immunoprecipitation (ChIP) assay. Estradiol also induced the EMT in hEECs by promoting HGF production. Furthermore, human mesothelial cells underwent the mesothelial-to-mesenchymal transition (MMT) during culture with estradiol-stimulated hEEC conditioned medium. Importantly, estradiol itself did not induce the MMT, and the estradiol-stimulated hEEC-conditioned medium in the presence of HGF antibodies reversed the MMT process. These results, which were obtained using immortalized hEECs, indicate that estradiol-induced HGF production may play a crucial role in the peritoneal implantation of human endometriotic cells by exerting proliferative and invasive effects via the EMT in hEECs and promoting the MMT in mesothelial cells.epithelial-to-mesenchymal transition; endometriosis; estradiol; hepatocyte growth factor; mesothelial-to-mesenchymal transition ENDOMETRIOSIS IS A CHRONIC, BENIGN DISEASE characterized by the presence of endometrium-like tissue outside the uterine cavity primarily on the ovaries or pelvic peritoneum. This condition is a major cause of symptoms, such as pelvic pain, dysmenorrhea, dyspareunia, and infertility, that affect 6 -10% of females of reproductive age and at least one-third of women with infertility and often relapses after surgery (9 -11). Although endometriosis was first described in 1860 (33), the etiology and pathogenesis of this disorder remain unclarified.

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Estradiol modulation of hepatocyte growth factor by stromal fibroblasts in the female reproductive tract

Cited by 25 publications

References 29 publications

Endocrine Regulation of the Mucosal Immune System in the Female Reproductive Tract

Endocrine Regulation of the Mucosal Immune System in the Female Reproductive Tract

The Role of Sex Hormones and the Tissue Environment in Immune Protection Against HIV in the Female Reproductive Tract

Estradiol-mediated hepatocyte growth factor is involved in the implantation of endometriotic cells via the mesothelial-to-mesenchymal transition in the peritoneum

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