Estrogen differentially regulates transcriptional landscapes of preoptic and arcuate kisspeptin neuron populations

Göcz, Balázs; Takács, Szabolcs; Skrapits, Katalin; Rumpler, Éva; Solymosi, Norbert; Póliska, Szilárd; Colledge, William H.; Hrabovszky, Erik; Sárvári, Miklós

doi:10.3389/fendo.2022.960769

Cited by 17 publications

(28 citation statements)

References 56 publications

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Section: Kisspeptin Neurons and Feedback By E2mentioning

confidence: 99%

“…A recent murine study shed light on how KP-neurons respond divergently to high and low E2 concentrations, by revealing differential RNA transcriptional responses between KP-neurons in the ARC and the RP3V ( 13 ). They identified 1583 oestrogen-responsive genes (majority suppressed) within the ARC, and 222 genes (majority upregulated) in the RP3V, thus showing that there are more oestrogen-responsive genes in the ARC than RP3V ( 13 ). Interestingly, whilst Esr1 (which encodes ERα) expression was increased in both RP3V and ARC KP-neurons, no differences in Esr2 (which encodes ERβ) or Gper1 (which encodes G-protein coupled oestrogen receptor) were observed ( 13 ).…”

Section: Kisspeptin Neurons and Feedback By E2mentioning

confidence: 99%

“…They identified 1583 oestrogen-responsive genes (majority suppressed) within the ARC, and 222 genes (majority upregulated) in the RP3V, thus showing that there are more oestrogen-responsive genes in the ARC than RP3V ( 13 ). Interestingly, whilst Esr1 (which encodes ERα) expression was increased in both RP3V and ARC KP-neurons, no differences in Esr2 (which encodes ERβ) or Gper1 (which encodes G-protein coupled oestrogen receptor) were observed ( 13 ). Furthermore, ERα interacted with 8 of 70 E2-dependent transcription factors within the ARC, but 0 of 10 E2-dependent transcription factors within RP3V ( 13 ).…”

Section: Kisspeptin Neurons and Feedback By E2mentioning

confidence: 99%

“…Interestingly, whilst Esr1 (which encodes ERα) expression was increased in both RP3V and ARC KP-neurons, no differences in Esr2 (which encodes ERβ) or Gper1 (which encodes G-protein coupled oestrogen receptor) were observed ( 13 ). Furthermore, ERα interacted with 8 of 70 E2-dependent transcription factors within the ARC, but 0 of 10 E2-dependent transcription factors within RP3V ( 13 ). Despite disparate E2-regulation, RP3V and ARC KP-neurons displayed 96 overlapping genes, with changes in 62 genes being analogous (e.g.…”

Section: Kisspeptin Neurons and Feedback By E2mentioning

confidence: 99%

“…Ghsr, Pgr, Npr2, Gad2, Calm1, Pcp4 ), and 34 genes being regulated in a contrasting manner (e.g. Kiss1, Vgf, Chrna7, Tmem35a ) ( 13 ). Overall, these data indicate that the effects of oestrogens are predominantly mediated by ERα in both the ARC and RP3V neurons, and that there are more oestrogen responsive genes in the ARC (mediating negative feedback) than in the RP3V (mediating positive feedback) ( 13 ).…”

Section: Kisspeptin Neurons and Feedback By E2mentioning

confidence: 99%

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Kisspeptin-neuron control of LH pulsatility and ovulation

et al. 2022

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Feedback from oestradiol (E2) plays a critical role in the regulation of major events in the physiological menstrual cycle including the release of gonadotrophins to stimulate follicular growth, and the mid-cycle luteinising hormone (LH) surge that leads to ovulation. E2 predominantly exerts its action via oestrogen receptor-alpha (ERα), however, as gonadotrophin releasing hormone (GnRH) neurons lack ERα, E2-feedback is posited to be indirectly mediated via upstream neurons. Kisspeptin (KP) is a neuropeptide expressed in hypothalamic KP-neurons that control GnRH secretion and plays a key role in the central mechanism regulating the hypothalamic-pituitary-gonadal (HPG) axis. In the rodent arcuate (ARC) nucleus, KP is co-expressed with Neurokinin B and Dynorphin; and thus, these neurons are termed ‘Kisspeptin-Neurokinin B-Dynorphin’ (KNDy) neurons. ARC KP-neurons function as the ‘GnRH pulse generator’ to regulate GnRH pulsatility, as well as mediating negative feedback from E2. A second KP neuronal population is present in the rostral periventricular area of the third ventricle (RP3V), which includes anteroventral periventricular (AVPV) nucleus and preoptic area neurons. These RP3V KP-neurons mediate positive feedback to induce the mid-cycle luteinising hormone (LH) surge and subsequent ovulation. Here, we describe the role of KP-neurons in these two regions in mediating this differential feedback from oestrogens. We conclude by considering reproductive diseases for which exploitation of these mechanisms could yield future therapies.

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Section: Kisspeptin Neurons and Feedback By E2mentioning

confidence: 99%

Section: Kisspeptin Neurons and Feedback By E2mentioning

confidence: 99%

Section: Kisspeptin Neurons and Feedback By E2mentioning

confidence: 99%

Section: Kisspeptin Neurons and Feedback By E2mentioning

confidence: 99%

Section: Kisspeptin Neurons and Feedback By E2mentioning

confidence: 99%

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Kisspeptin-neuron control of LH pulsatility and ovulation

et al. 2022

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Defining hippocampal area CA2 in the fox (Vulpes vulpes) brain

et al. 2023

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Since 1959, the Russian Farm-Fox study has bred foxes to be either tame or, more recently, aggressive, and scientists have used them to gain insight into the brain structures associated with these behavioral features. In mice, hippocampal area CA2 has emerged as one of the essential regulators of social aggression, and so to eventually determine whether we could identify differences in CA2 between tame and aggressive foxes, we first sought to identify CA2 in foxes (Vulpes vulpes). As no clearly defined area of CA2 has been described in species such as cats, dogs, or pigs, it was not at all clear whether CA2 could be identified in foxes. In this study, we cut sections of temporal lobes from male and female red foxes, perpendicular to the long axis of the hippocampus, and stained them with markers of CA2 pyramidal cells commonly used in tissue from rats and mice. We observed that antibodies against Purkinje cell protein 4 best stained the pyramidal cells in the area spanning the end of the mossy fibers and the beginning of the pyramidal cells lacking mossy fibers, resembling the pattern seen in rats and mice. Our findings indicate that foxes do have a "molecularly defined" CA2, and further, they suggest that other carnivores like dogs and cats might as well. With this being the case, these foxes could be useful in future studies looking at CA2 as it relates to aggression.

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