Estrogen inhibits calbindin-D28k expression in mouse uterus.

Abstract

The cellular localization and hormonal controls of calbindin-D9k expression in the rodent reproductive tract have suggested new functions for this protein. The present studies were undertaken to extend the earlier studies of calbindin-D9k to the related protein, calbindin-D28k. Immunohistochemical studies revealed that calbindin-D28k was absent from female rat reproductive tissues, but was abundantly expressed in immature mouse uterus and oviduct. Immunoreactivity was restricted to the endometrial and glandular epithelium of the uterus and the oviductal epithelium. Neither 1,25-dihydroxyvitamin D- nor strontium-containing diets (to blunt 1,25-dihydroxyvitamin D production) affected expression of calbindin-D28k. Uterine, but not oviductal, calbindin-D28k decreased markedly at sexual maturity; this pattern persisted in pregnant mice and was reproduced in immature mice by the administration of estradiol (3 micrograms/day for 3 days). RNA extraction and Northern analyses demonstrated that estrogen markedly decreased calbindin-D28k mRNA abundance in the uterus, but not in the oviduct. These findings suggest that estrogen affects mammalian calbindin-D28k expression and represent a rare example of estrogen-induced down-regulation of gene expression.

Tissue interactions with underlying dura mater inhibit osseous obliteration of developing cranial sutures

“…The final numbers of transplants in each group are given in the tables. Results were tested by three-way analysis of variance with Bonferoni correction for post-hoc tests as previously described (Opperman et al, 1992). All measurements are in micrometers and data presented as means * SEM.…”

Dominant expression of porcine Calbindin‐D9k in the uterus during a luteal phase

“…In the intestine, CaBP-9k is regulated at the transcriptional level and posttranscriptional level by 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3), the hormonal form of vitamin D, and is involved in intestinal calcium absorption (Roche et al, 1986;Wasserman and Taylor, 1966). The expression and regulation of uterine CaBP-9k have been shown in the uterus of chickens (Corradino et al, 1993), rats (Bruns et al, 1988) and mice (Opperman et al, 1992), and uterine CaBP-9k is dominantly expressed in the endometrium and myometrium (Darwish et al, 1991). In a previous study, the porcine CaBP-9k cDNA was cloned by anchored polymerase chain reaction, indicating its full-length coding region of 79 amino acids, 57 nucleotides 5 0 and 149 nucleotides 3 0 noncoding region (Jeung et al, 1992a,b).…”

Homologous Up-Regulation of Vitamin D Receptors Is Tissue Specific in the Rat

“…48), whereas CaBP-D28 is down-regulated by estrogen. (49) Similarly, CaBP-D28 in chick egg shell gland is up-regulated by estrogen, but not 1,25(OH) 2 D 3 . (50) The CaBP-D9 is also present in lung and placenta, but is not vitamin D-regulated in those tissues.…”

“…(50) The CaBP-D9 is also present in lung and placenta, but is not vitamin D-regulated in those tissues. (40,49,51) Finally, the CaBP-D's are absent regardless of vitamin D status in several other targets, including in particular testis and heart. (52) Thus, many proteins, including the calbindins-D, demonstrate tissue-selective localization and tissue-and hormone-specific patterns of regulation.…”