Estrogen receptor immunoreactivity in prepubertal and adult male Syrian hamsters

Romeo, Russell D.; Diedrich, Stefani L.; Sisk, Cheryl L.

doi:10.1016/s0304-3940(99)00233-5

Cited by 15 publications

(10 citation statements)

References 17 publications

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“…An alternative explanation for the decreased number of AR-ir cells in the MPN of males exposed to gonadal hormones during puberty is a steroid-dependent loss of AR-containing cells. Such putative cell death would appear to be specific to the AR-containing cells, as we did not observe an equivalent decrease in ER␣-ir cell number, which is in agreement with our previous finding that prepubertal and adult male castrates have similar numbers of ER␣-ir cells in hypothalamic and amygdaloid nuclei (Romeo et al, 1999). Furthermore, since about half of the AR-containing cells in the MPN are also ER␣-positive (Wood and Newman, 1995a), any loss of AR-ir cells is likely to be specific to the subpopulation of neurons that contain only ARs.…”

Section: Discussionsupporting

confidence: 92%

Effects of gonadal steroids during pubertal development on androgen and estrogen receptor-? immunoreactivity in the hypothalamus and amygdala

2000

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Perinatal development is often viewed as the major window of time for organization of steroid‐sensitive neural circuits by steroid hormones. Behavioral and neuroendocrine responses to steroids are dramatically different before and after puberty, suggesting that puberty is another window of time during which gonadal steroids affect neural development. In the present study, we investigated whether the presence of gonadal hormones during pubertal development affects the number of androgen receptor and estrogen receptor α‐immunoreactive (AR‐ir and ERα‐ir, respectively) cells in limbic regions. Male Syrian hamsters were castrated either before or after pubertal development, and 4 weeks later they received a single injection of testosterone or oil vehicle 4 h prior to tissue collection. Immunocytochemistry for AR and ERα was performed on brain sections from testosterone‐treated and oil‐treated males, respectively. Adult males that had been castrated before puberty had a greater number of AR‐ir cells in the medial preoptic nucleus than adult males that had been castrated after puberty. There were no significant differences in ERα‐ir cell number in any of the brain regions examined. The demonstration that exposure to gonadal hormones during pubertal development is associated with reduced AR‐ir in the medial preoptic nucleus indicates that puberty is a period of neural development during which hormones shape steroid‐sensitive neural circuits. © 2000 John Wiley & Sons, Inc. J Neurobiol 44: 361–368, 2000

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Section: Discussionsupporting

confidence: 92%

Effects of gonadal steroids during pubertal development on androgen and estrogen receptor-? immunoreactivity in the hypothalamus and amygdala

2000

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“…Loss of adrenal medullary ERα‐LI during puberty in the male may be a general developmental process because it also occurs in other tissues, such as the pituitary in the rat (Thieulant et al ., 1982), ventral medial hypothalamus in the hamster (Romeo et al ., 1999) and prostate in the guinea pig (Tilley et al ., 1987; Tilley et al ., 1989). Whilst the mechanism for this effect is not known, in this study we have shown that exposure to adult levels of androgens in prepubertal males produces an early loss in ERα‐LI, similar to the androgen‐induced loss from guinea pig prostate (Tilley et al ., 1987; Tilley et al ., 1989) and ovarian tissue (Saiduddin & Zassenhaus, 1978).…”

Section: Discussionmentioning

confidence: 99%

Role of adrenal medulla in development of sexual dimorphism in inflammation

Green

Rantapää-Dahlqvist

Isenberg

et al. 2001

Eur J of Neuroscience

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Many inflammatory diseases show a female predilection in adults, but not prepubertally. Because sex differences in the inflammatory response in the adult rat are mediated, in part, by sexual dimorphism in adrenal medullary function, we investigated the contribution of the adrenal medulla to the ontogeny of sexual dimorphism in inflammation. Whilst there was no sex difference in the magnitude of the plasma extravasation (PE) induced by the potent inflammatory mediator bradykinin (BK) in prepubertal rats, in adult rats BK-induced PE was markedly greater in males. Also, adult male rats, gonadectomized prior to puberty, had a lower magnitude of BK-induced PE than did adult male controls, whilst adult females gonadectomized prepubertally had higher BK-induced PE than did controls. In rats gonadectomized after puberty, the magnitude of BK-induced PE in adult males was not affected, whilst in females it resulted in significantly higher BK-induced PE, similar to the effect of prepubertal gonadectomy. When tested prepubertally, adrenal denervation increased the magnitude of BK-induced PE in females, but not in males. In contrast, in both males and females tested as adults, but castrated prepubertally, and in gonad-intact adult females, adrenal denervation significantly increased the magnitude of BK-induced PE. Adrenal denervation in prepubertal females given adult levels of 17beta-oestradiol produced a marked enhancement in the denervation-induced increase in magnitude of BK-induced PE compared to females not exposed prematurely to sex hormones. These studies suggest that an adrenal medulla-dependent inhibition of BK-induced PE is present in female but not male rats, and is enhanced by oestrogen but suppressed by testosterone.

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“…Alternatively, the effects of testosterone could be mediated by an oestrogen receptor mechanism. Testosterone is readily converted to oestrogen in the brain and the hamster MPN is rich in oestrogen receptors, but there are relatively few oestrogen receptor‐immunoreactive neurones in the lateral septum (29). This could explain the differential regulation of the V 1a receptor in the MPN and the lateral septum.…”

Section: Methodsmentioning

confidence: 99%

Vasopressin (V_1a) Receptor Binding, mRNA Expression and Transcriptional Regulation by Androgen in the Syrian Hamster Brain

Young

Wang

Cooper³

et al. 2000

J Neuroendocrinology

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Arginine vasopressin plays an important role in the regulation of social behaviours in rodents. In the Syrian hamster, vasopressin injected directly into the brain stimulates scent marking and aggressive behaviour in a steroid dependent manner and is therefore a useful model for investigating steroidpeptide±behaviour interactions. In this study, we used in situ hybridization and radioligand binding assays on adjacent sections of hamster brains to compare the relative distribution of vasopressin (V 1a ) receptor mRNA and V 1a receptor binding. V 1a receptor mRNA and binding are abundant in the lateral septum, bed nucleus of the stria terminalis, medial preoptic nucleus, anterodorsal thalamus and suprachiasmatic nucleus. Moderate receptor binding and low levels of receptor mRNA are present in the central nucleus of the amygdala and a lateral zone from the medial preoptic area through the anterior hypothalamus. V 1a receptor mRNA is anatomically more restricted in several areas compared to the ligand binding pattern, which is consistent with signi®cant spread of receptor protein along neuronal processes. Comparison of V 1a receptor ligand binding and mRNA in intact, castrated, and castrated-testosterone treated animals reveals that V 1a receptors in the medial preoptic nucleus are regulated by androgen, most likely by an upregulation of V 1a receptor gene expression in a cluster of neurones concentrated in the ventromedial part of this nucleus. This study con®rms the presence of the V 1a subtype of vasopressin receptors in behaviourally important regions of the hamster brain and suggests that transcriptional regulation by gonadal steroids may play a role in modulating behavioural sensitivity to vasopressin.Centrally released arginine vasopressin plays an important role in the regulation of several rodent social behaviours, such as social recognition (1. ), scent marking (2. ), aggression (3, 4. ), pair bonding (4. ), paternal care (5. ) and af®liation (6. ). In the Syrian hamster (Mesocricetus auratus), vasopressin induces a form of scent marking behaviour called¯ank marking (2. ), as well as aggression (3. ). Vasopressin injected directly into a zone from the medial and lateral aspects of the medial preoptic area through the posterior medial and lateral aspects of the anterior hypothalamic area (MPOA-AH) stimulates high levels of¯ank marking and aggressive behaviour, while a speci®c vasopressin antagonist suppresses these behaviours (7, 8. ). These effects of vasopressin appear to be mediated by a vasopressin (V 1 ) subtype receptor since vasopressin and oxytocin analogs known to interact speci®cally with the V 1 receptor stimulate, while the speci®c V 1 receptor antagonist d(CH 2 ) 5 Tyr(Me)AVP inhibits¯ank marking in a dose dependent manner (8, 9. ). Both vasopressin-immunoreactive neurones and V 1a receptors are found within the vasopressinresponsive region of the MPOA-AH (7, 10, 11. ).Flank marking behaviour and the behavioural response to centrally injected vasopressin in the hamster are sensitive to...

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Estrogen receptor immunoreactivity in prepubertal and adult male Syrian hamsters

Cited by 15 publications

References 17 publications

Effects of gonadal steroids during pubertal development on androgen and estrogen receptor-? immunoreactivity in the hypothalamus and amygdala

Effects of gonadal steroids during pubertal development on androgen and estrogen receptor-? immunoreactivity in the hypothalamus and amygdala

Role of adrenal medulla in development of sexual dimorphism in inflammation

Vasopressin (V_1a) Receptor Binding, mRNA Expression and Transcriptional Regulation by Androgen in the Syrian Hamster Brain

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Estrogen receptor immunoreactivity in prepubertal and adult male Syrian hamsters

Cited by 15 publications

References 17 publications

Effects of gonadal steroids during pubertal development on androgen and estrogen receptor-? immunoreactivity in the hypothalamus and amygdala

Effects of gonadal steroids during pubertal development on androgen and estrogen receptor-? immunoreactivity in the hypothalamus and amygdala

Role of adrenal medulla in development of sexual dimorphism in inflammation

Vasopressin (V1a) Receptor Binding, mRNA Expression and Transcriptional Regulation by Androgen in the Syrian Hamster Brain

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Vasopressin (V_1a) Receptor Binding, mRNA Expression and Transcriptional Regulation by Androgen in the Syrian Hamster Brain