Estrogenic Activities of Fatty Acids and a Sterol Isolated from Royal Jelly

Suzuki, Koji; Isohama, Yoichiro; Maruyama, Haruhiko; Yamada, Yayoi; Narita, Yukio; Ohta, Shozo; Araki, Yasufumi; Miyata, Takeshi; Mishima, Satoshi

doi:10.1093/ecam/nem036

Cited by 99 publications

(93 citation statements)

References 27 publications

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“…Exposure of immature rats to these compounds by subcutaneous injection induced mild hypertrophy of the luminal epithelium of the uterus, but was not associated with an increase in uterine weight. These findings provide evidence that these compounds contribute to the estrogenic effect of RJ (30).…”

Section: ) Effects On Bone Metabolismmentioning

confidence: 54%

Pharmacological Basis of Traditional Medicines and Health Supplements as Curatives

Miyata

2007

J Pharmacol Sci

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Abstract. Traditional Oriental medicines and health supplements have been empirically used to treat various ailments but most of them have not been evaluated objectively to prove their efficacies. We have been investigating the medical benefits of traditional Oriental medicines and health supplements as alternatives and their varied actions and mechanisms by pharmacological approaches. The study on airway inflammation has shown that even a Kampo preparation, Bakumondo-to, has anti-inflammatory, anti-allergic, immunomodulatory, secretory-modulating, and metabolic regulatory actions. All of its actions are based on the restoration of normal molecular and cellular functions through DNA transcriptional regulation. In other previous studies, we showed that a health supplement, royal jelly (RJ) has weak estrogenic activity. RJ competes with 17β-estradiol for binding to the human estrogen receptors α and β, although it is much weaker than diethylstilbestrol in binding affinity. Treatment of MCF-7 cells with RJ enhances proliferation, and concomitant treatment with tamoxifen blocked this effect. A reporter gene assay showed that RJ enhanced transcription of the luciferase gene through the estrogenresponsive element in MCF-7 cells. Furthermore, subcutaneous injection of RJ restored the expression of vascular endothelial growth factor gene in the uteri of ovariectomized rats. We suggest that the diverse pharmacological functions of RJ can be ascribed, in part, to its estrogenic effects. We hypothesize that traditional medicine, which has multiple actions, may be better than Western medicine of a single component to treat various diseases including "Mibyou" (presymptomatic disease). Our findings provide new ideas about the nature of disorders and diseasestate development that involve complicated mechanisms and will contribute to novel principles to prevent diseases and establish new treatments. Adoption of the means of translational research should provide an objective background for efficacy and stimulate broader application and usage of traditional medicines and health supplements.

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Section: ) Effects On Bone Metabolismmentioning

confidence: 54%

Pharmacological Basis of Traditional Medicines and Health Supplements as Curatives

Miyata

2007

J Pharmacol Sci

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“…It was identified that RJ stimulated the expression of endogenous estrogen-responsive genes [presenilin 2 and vascular endothelial growth factor (VEGF)] by activating estrogen receptors in MCF-7 cells, and RJ could restore the expression of VEGF in uterus of rats that received bilateral ovariectomy (13). Experimental results demonstrated that four compounds from RJ can improve the transcription of a reporter gene, which contained an estrogen-responsive element, and subcutaneously injecting immature rats with those compounds for 23 days induced mild hypertrophy in the luminal epithelium of the rat uterus (14). With evidence that it was an etiological cause of breast cancer, estrogen attracted numerous studies on its association with breast carcinogenesis (15).…”

Section: Introductionmentioning

confidence: 99%

The effect of royal jelly on the growth of breast cancer in mice

et al. 2017

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Abstract. Due to various pharmacological properties, including antioxidative, anti-inflammatory and antibiotic properties, royal jelly (RJ) has been widely consumed in daily diets in numerous countries. In the present study, the effect of RJ on 4T1-bearing mice was investigated. The study was performed by feeding 4T1-bearing mice with RJ using either the prophylactic-therapeutic (PTRJ) or therapeutic (TRJ) method. The experimental results for the PTRJ group demonstrated that the weight of tumor was significantly reduced (RJ 0.5 and 1.5 g/kg); and in the serum, the levels of interleukin (IL)-2 (RJ 0.5 and 1.5 g/kg), interferon (IFN)-α, superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) were significantly elevated, but the concentrations of IL-4 (RJ 0.5 and 1.5 g/kg) and IL-10 (RJ 1.0 g/kg) were significantly decreased. In addition, the activities of T-AOC and glutathione reductase (GR) were significantly improved in the liver, whereas in the kidney, the activities of T-AOC and GR were significantly increased only under the dose of 0.5 g/kg. For the TRJ group, the antitumor effect of RJ was not significant; the change in IL-2, IFN-α, SOD and T-AOC levels in the serum, and the change in T-AOC and GR in liver were similar to those observed in the PTRJ groups. RJ treatment was demonstrated to reduce the development of breast tumor in mice, and simultaneously improve the antioxidant capacity of the serum, liver and kidney, particularly using the prophylactic-therapeutic method. These results corroborated the efficacy of RJ supplementation in diets. The results of the present study suggest that the antioxidant and immunomodulatory activities of RJ serve an important role on antitumor growth.

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“…A atividade estrogênica apresentada pela geleia real (Mishima et al, 2005;Suzuki et al, 2008) nas doses utilizadas, provavelmente, não foi suficiente para promover diferenças no desenvolvimento do aparelho genital das fêmeas e, consequentemente, em seus pesos. O IGS corresponde à proporção do peso corporal alocada em ambos os ovários, sendo um dado auxiliar para a observação da influência da geleia real sobre o aparelho genital das fêmeas.…”

Section: Methodsunclassified

Morfometria do aparelho genital e resposta superovulatória de coelhas suplementadas com geleia real

Dutra

Barbosa

Santana

et al. 2013

Arq. Bras. Med. Vet. Zootec.

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Avaliou-se o efeito da suplementação com geleia real sobre a morfometria do aparelho genital, resposta superovulatória e qualidade embrionária de coelhas. Trinta e seis fêmeas foram distribuídas em quatro grupos (G), sendo: G1 (n=9) formado por animais não suplementados com geleia real, e G2, G3 e G4 (n=9 em cada grupo) por animais suplementados com 10, 20 e 40mg/dia de geleia real. A superovulação consistiu na aplicação de 40UI de gonadotrofina coriônica equina, seguida por 40UI de gonadotrofina coriônica humana, via intramuscular, 48 horas após, e submetidas à cobrição natural. Os animais foram sacrificados, e os embriões coletados 72 horas após a cópula. Não houve diferença estatística entre tratamentos para as variáveis analisadas. O peso médio do aparelho genital foi de 10,88±0,38g; dos ovários - direito e esquerdo -, 0,28±0,02g; e o índice gonadossomático, 0,02±0,0g. O número médio de estruturas totais recuperadas foi de 9,2±1,4; de embriões viáveis, 8,7±1,4; e de degenerados, 0,5±0,2. Dos embriões viáveis, 5,6±0,8 foram classificados como grau I; 2,3±0,5, como grau II; e 0,8±0,2, como grau III. A suplementação com geleia real na dose de até 40mg/dia não apresentou efeito estimulador sobre o aparelho genital e a qualidade embrionária de coelhas.

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Estrogenic Activities of Fatty Acids and a Sterol Isolated from Royal Jelly

Cited by 99 publications

References 27 publications

Pharmacological Basis of Traditional Medicines and Health Supplements as Curatives

Pharmacological Basis of Traditional Medicines and Health Supplements as Curatives

The effect of royal jelly on the growth of breast cancer in mice

Morfometria do aparelho genital e resposta superovulatória de coelhas suplementadas com geleia real

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