Estructura y dinámica del bacterioplancton en cuatro lagunas pampeanas de la cuenca del río Salado (Buenos Aires, Argentina)

Nuozzi, Guillermina; Rocha, Camila Seoane; Sagua, Mara; Llames, María Eugenia del Rosario; Huber, Paula; Metz, Sebastián; Lagomarsino, Leonardo; Schiaffino, M. Romina

doi:10.25260/ea.22.32.2.0.1811

Cited by 4 publications

(4 citation statements)

References 59 publications

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“…abundance. The second set had been previously used for NGS of 16 rRNA gene by Illumina MiSeq platform to investigate the composition of planktonic bacteria (Seone Rocha 2018;Quiroga 2020;Schia no et al 2020;Nuozzi et al 2022). In the current study, only the Legionella reads obtained from NGS data were utilized.…”

Section: Molecular Approachesmentioning

confidence: 99%

“…The partial 16S rRNA gene was sequenced by Illumina MiSeq 2 x 300 paired-end sequencing (Macrogen Inc., Corea), using the primers 341F/805R (Herlemann et al 2011), which cover the hypervariable regions V3-V4. After quality control, reads were analyzed using three different sequence processing algorithms: the UPARSE algorithm (Edgar 2013) that de ned Operational Taxonomic Units clustered to a 97% similarity threshold (97% OTUs) as depicted in Seone Rocha (2018), the UNOISE algorithm (Edgar 2016) de ning zero-radius OTUs (zOTUs) as shown in Quiroga et al ( 2020) and Schia no et al ( 2020) and clustering of sequences using the DADA2 methodology (Callahan et al 2016) to obtain Amplicon Sequence Variants (ASVs), as shown in Nuozzi et al (2022).…”

Section: )mentioning

confidence: 99%

“…As mentioned above, qPCR data was compared with a subset of NGS reads from previous studies, employing different processing methodologies: OTUs with a 97% similarity threshold (Seoane Rocha 2018), zero-radius OTUs (Quiroga 2020; Schia no et al 2020) and ASVs (Nuozzi et al 2022). It becomes evident that each bioinformatic analysis method places different importance on species distinctiveness, overall diversity and delity.…”

Section: Comparative Of Ngs and Qpcr Abundancesmentioning

confidence: 99%

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Temporal dynamics of Legionella (Proteobacteria, Legionellaceae) in two Pampean shallow lakes from Argentina

Bianchelli,

Sagua,

Quiroga

et al. 2024

Preprint

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Aquatic systems have traditionally played a key role in the development of human life, providing multiple ecosystem services to society and being a reservoir for a wide biodiversity of organisms. Among them, bacteria belonging to Legionella stand out, mainly because they are of great interest both in the field of microbial ecology and public health, since some of them turn out to be pathogenic for humans. The aim of this work was to study the monthly temporal dynamics of Legionella spp. and its relationship with the environmental variables measured in two Pampean shallow lakes (Gómez and Carpincho, Buenos Aires Province, Argentina). The analysis was carried out using a quantitative approach by real-time Polymerase Chain Reaction (qPCR) and a non-quantitative approach using bacterial diversity data obtained by Next Generation Sequencing (NGS), using the Illumina MiSeq platform. Our results showed that the overall Legionella abundance was very high in the studied Pampean shallow lakes. Notably, fluctuations in dissolved organic carbon and temperature influenced the dynamics shifts in Legionella abundances. Correlation analyses between Legionella reads from NGS and copy numbers obtained through qPCR revealed positive relationships, unveiling distinctions attributable to the diverse sequence processing algorithms employed in the analysis of NGS data.

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Section: Molecular Approachesmentioning

confidence: 99%

Section: )mentioning

confidence: 99%

Section: Comparative Of Ngs and Qpcr Abundancesmentioning

confidence: 99%

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Temporal dynamics of Legionella (Proteobacteria, Legionellaceae) in two Pampean shallow lakes from Argentina

Bianchelli,

Sagua,

Quiroga

et al. 2024

Preprint

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show abstract

“…Regional underground runoff of highly saline water, resulting from the presence of soluble minerals in the sediments, is discharged in the main basins, while local runoff translates into small shallow lakes with fresh water (Kruse and Rojo 1991). During drought periods, the number of lakes in the area decreased, the salinity of the water increased, and numerous shallow lakes disappeared (Quiroz et al 2003).…”

Section: Study Area: Environment and Archaeological Backgroundmentioning

confidence: 99%

Systemic stress in hunter-gatherers from the Central Pampas Dunefields (Argentina): evaluating linear enamel hypoplasia during the Holocene

Marini

Flensborg

2023

Archaeol Anthropol Sci

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This article aims to evaluate whether the socio-environmental changes observed in the Central Pampas Dune elds (Argentina) throughout the Holocene (ca. 11.700-350 cal yr BP) caused episodes of systemic stress in hunter-gatherer populations. Linear enamel hypoplasia was analyzed macroscopically with a 40X magnifying glass and oblique light in 337 teeth from 26 adult individuals recovered from 11 archaeological sites. Data were analyzed following three levels of observation: individual, tooth, and number of dental enamel lines. 42.3% of the individuals analyzed presented linear enamel hypoplasia.The lines affected males and females similarly. A higher frequency was observed in the Late Holocene (ca. 4200-350 cal yr. BP) about earlier periods. The age of growth-disruption events occurred between 2 and 6.05 years, and stress events are observed at earlier ages in the Late Holocene than in the Middle Holocene. The causes could be related to metabolic stress associated with weaning, variations in the availability of natural resources over time, development of infectious and parasitic diseases during changes in social organization strategies in the Late Holocene, and gastrointestinal diseases by water intake in poor conditions. Comparison at the level of the Pampas region indicates moderate to high values of linear enamel hypoplasia, suggesting the exposure of hunter-gatherer groups to recurrent episodes of stress during the early stages of postnatal life.

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Temporal dynamics of Legionella (Proteobacteria, Legionellaceae) in two Pampean shallow lakes from Argentina

Bianchelli,

Sagua,

Quiroga

et al. 2024

Environ Sci Pollut Res

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Estructura y dinámica del bacterioplancton en cuatro lagunas pampeanas de la cuenca del río Salado (Buenos Aires, Argentina)

Cited by 4 publications

References 59 publications

Temporal dynamics of Legionella (Proteobacteria, Legionellaceae) in two Pampean shallow lakes from Argentina

Temporal dynamics of Legionella (Proteobacteria, Legionellaceae) in two Pampean shallow lakes from Argentina

Systemic stress in hunter-gatherers from the Central Pampas Dunefields (Argentina): evaluating linear enamel hypoplasia during the Holocene

Temporal dynamics of Legionella (Proteobacteria, Legionellaceae) in two Pampean shallow lakes from Argentina

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