ESTs library from embryonic stages reveals tubulin and reflectin diversity in Sepia officinalis (Mollusca — Cephalopoda)

Bassaglia, Yann; Bekel, Thomas; Silva, Corinne Da; Poulain, Julie; Andouche, Aude; Navet, Sandra; Bonnaud, Laure

doi:10.1016/j.gene.2012.01.100

Cited by 23 publications

(23 citation statements)

References 54 publications

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“…General descriptions of eye development in various cephalopod species have been documented, but an in-depth molecular and cellular understanding of major morphogenetic and cell differentiation events is lacking (Arnold, 1965(Arnold, , 1966(Arnold, , 1967Arnold and Williams-Arnold, 1976;Gilbert et al, 1990;Marthy, 1973;Yamamoto, 1985;Yamamoto et al, 1985;Naef, 1928). Recently, the cephalopod genomic infrastructure was greatly improved by publication of the Octopus bimaculoides genome and a few transcriptomic databases Alon et al, 2015;Yoshida, 2011;Wollesen et al, 2014;Bassaglia et al, 2012). Despite these improvements, however, few sequencing efforts have informed our understanding of embryonic development or organogenesis.…”

Section: Introductionmentioning

confidence: 99%

Eye development and photoreceptor differentiation in the cephalopod Doryteuthis pealeii

et al. 2016

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Photoreception is a ubiquitous sensory ability found across the Metazoa, and photoreceptive organs are intricate and diverse in their structure. Although the morphology of the compound eye in Drosophila and the single-chambered eye in vertebrates have elaborated independently, the amount of conservation within the 'eye' gene regulatory network remains controversial, with few taxa studied. To better understand the evolution of photoreceptive organs, we established the cephalopod Doryteuthis pealeii as a lophotrochozoan model for eye development. Utilizing histological, transcriptomic and molecular assays, we characterize eye formation in Doryteuthis pealeii. Through lineage tracing and gene expression analyses, we demonstrate that cells expressing Pax and Six genes incorporate into the lens, cornea and iris, and the eye placode is the sole source of retinal tissue. Functional assays demonstrate that Notch signaling is required for photoreceptor cell differentiation and retinal organization. This comparative approach places the canon of eye research in traditional models into perspective, highlighting complexity as a result of both conserved and convergent mechanisms.

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Section: Introductionmentioning

confidence: 99%

Eye development and photoreceptor differentiation in the cephalopod Doryteuthis pealeii

et al. 2016

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“…Reflectins are compositionally different from the reflective elements found in other marine and aquatic organisms, which are mostly composed of the purine crystals guanine and hypoxanthine 10, 11. Proteins found in iridophores of the squid L. pealeii and RNA transcripts from the embryos of the cuttlefish Sepia officinalis have recently been shown to share homology with the reflectin family of proteins,15 suggesting that reflectin proteins may represent a ubiquitous, yet novel, optical nanostructural material among cephalopods 11, 12. Analysis of reflectin sequences in E. scolopes revealed that reflectin proteins are encoded by at least six genes in three subfamilies and possess five repeating domains, which are well‐conserved among members of the family.…”

Section: Introductionmentioning

confidence: 99%

Recombinant reflectin‐based optical materials

Qin

Dennis

Zhang

et al. 2012

J Polym Sci B Polym Phys

109

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Reflectins are a unique group of structural proteins involved in dynamic optical systems in cephalopods that modulate incident light or bioluminescence. We describe cloning, structural characterization, and optical properties of a reflectin‐based domain, refCBA, from reflectin 1a of Hawaiian bobtail squid, Euprymna scolopes. Thin films created from the recombinant protein refCBA display interesting optical features when the recombinant protein is appropriately organized. RefCBA was cloned and expressed as a soluble protein enabling purification, with little structural organization found using Fourier transform infrared spectroscopy and circular dichroism. Single‐layer and multi‐layer thin films of refCBA were then produced by flow coating and spin coating, and displayed colors due to thin film interference. Diffraction experiments showed the assemblies were ordered enough to work as diffraction gratings to generate diffraction patterns. Nano‐spheres and lamellar microstructures of refCBA samples were observed by scanning electron microscopy and atomic force microscopy. Despite the reduced complexity of the refCBA protein compared to natural reflectins, unique biomaterials with similar properties to reflectins were generated by self‐assembled reflectin‐based refCBA molecules. © 2012 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys, 2013

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“…This study focused on three genes associated with early tissue speciation. Previous studies have identified many genes implicated in cephalopod limb morphogenesis; future experimentation would especially benefit from timepoint‐ and treatment‐specific profiles, with particular emphasis on genes associated with nervous precursor distribution and development of complex sensory and neurological structures (Baratte & Bonnaud, ; Bassaglia et al, ; Buresi, Baratte, Da Silva, & Bonnaud, ; Buresi, Canali, Bonnaud, & Baratte, ; Buresi, Croll, Tiozzo, Bonnaud, & Baratte, ; Focareta, Sesso, & Cole, ; Zhang & Tublitz, ), as well as muscle (Bassaglia et al, ; Fossati et al, ; Navet et al, ; Zullo et al, ) and endothelium (Focareta & Cole, ). Additional genes of interest should be garnered from the available studies in models across taxa (Fei et al, ; Godwin et al, ; Kawakami et al, ; Uygur & Lee, ; Wischin et al, ); ideally strategies utilizing transcriptomic or single‐cell RNA sequencing could be compared on both amputated and regenerated limb tips to provide greater clarity on intraindividual variation in expression based on injury‐naïve and regenerating conditions.…”

Section: Resultsmentioning

confidence: 99%

Reversion to developmental pathways underlies rapid arm regeneration in juvenile European cuttlefish, Sepia officinalis (Linnaeus 1758)

et al. 2019

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Coleoid cephalopods, including the European cuttlefish (Sepia officinalis), possess the remarkable ability to fully regenerate an amputated arm with no apparent fibrosis or loss of function. In model organisms, regeneration usually occurs as the induction of proliferation in differentiated cells. In rare circumstances, regeneration can be the product of naïve progenitor cells proliferating and differentiating de novo . In any instance, the immune system is an important factor in the induction of the regenerative response. Although the wound response is well‐characterized, little is known about the physiological pathways utilized by cuttlefish to reconstruct a lost arm. In this study, the regenerating arms of juvenile cuttlefish, with or without exposure at the time of injury to sterile bacterial lipopolysaccharide extract to provoke an antipathogenic immune response, were assessed for the transcription of early tissue lineage developmental genes, as well as histological and protein turnover analyses of the resulting regenerative process. The transient upregulation of tissue‐specific developmental genes and histological characterization indicated that coleoid arm regeneration is a stepwise process with staged specification of tissues formed de novo, with immune activation potentially affecting the timing but not the result of this process. Together, the data suggest that rather than inducing proliferation of mature cells, developmental pathways are reinstated, and that a pool of naïve progenitors at the blastema site forms the basis for this regeneration.

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ESTs library from embryonic stages reveals tubulin and reflectin diversity in Sepia officinalis (Mollusca — Cephalopoda)

Cited by 23 publications

References 54 publications

Eye development and photoreceptor differentiation in the cephalopod Doryteuthis pealeii

Eye development and photoreceptor differentiation in the cephalopod Doryteuthis pealeii

Recombinant reflectin‐based optical materials

Reversion to developmental pathways underlies rapid arm regeneration in juvenile European cuttlefish, Sepia officinalis (Linnaeus 1758)

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